Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS17600

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS17595 and RPSI07_RS17600 are separated by 115 nucleotidesRPSI07_RS17600 and RPSI07_RS17605 are separated by 249 nucleotides RPSI07_RS17595: RPSI07_RS17595 - asparaginase, at 2,150,531 to 2,151,526 _RS17595 RPSI07_RS17600: RPSI07_RS17600 - ferredoxin--NADP reductase, at 2,151,642 to 2,152,418 _RS17600 RPSI07_RS17605: RPSI07_RS17605 - transcriptional repressor LexA, at 2,152,668 to 2,153,318 _RS17605 Position (kb) 2151 2152 2153Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2150.851 kb on - strand, within RPSI07_RS17595at 2150.851 kb on - strand, within RPSI07_RS17595at 2151.009 kb on + strand, within RPSI07_RS17595at 2151.009 kb on + strand, within RPSI07_RS17595at 2151.010 kb on - strand, within RPSI07_RS17595at 2151.010 kb on - strand, within RPSI07_RS17595at 2151.565 kb on - strandat 2151.565 kb on - strandat 2151.643 kb on + strandat 2151.644 kb on - strandat 2152.515 kb on + strandat 2152.516 kb on - strandat 2152.516 kb on - strandat 2152.516 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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2,150,851 - RPSI07_RS17595 0.32 -2.0
2,150,851 - RPSI07_RS17595 0.32 +0.6
2,151,009 + RPSI07_RS17595 0.48 -0.2
2,151,009 + RPSI07_RS17595 0.48 -1.7
2,151,010 - RPSI07_RS17595 0.48 -1.3
2,151,010 - RPSI07_RS17595 0.48 -0.8
2,151,565 - +3.0
2,151,565 - -0.8
2,151,643 + -0.9
2,151,644 - -1.2
2,152,515 + +1.4
2,152,516 - +0.5
2,152,516 - +0.2
2,152,516 - +0.6

Or see this region's nucleotide sequence