Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS17580 and RPSI07_RS17585 are separated by 3 nucleotides RPSI07_RS17585 and RPSI07_RS17590 are separated by 65 nucleotides RPSI07_RS17590 and RPSI07_RS17595 are separated by 93 nucleotides RPSI07_RS17595 and RPSI07_RS17600 are separated by 115 nucleotides
RPSI07_RS17580: RPSI07_RS17580 - 30S ribosomal protein S18, at 2,149,386 to 2,149,673
_RS17580
RPSI07_RS17585: RPSI07_RS17585 - primosomal replication protein N, at 2,149,677 to 2,150,003
_RS17585
RPSI07_RS17590: RPSI07_RS17590 - 30S ribosomal protein S6, at 2,150,069 to 2,150,437
_RS17590
RPSI07_RS17595: RPSI07_RS17595 - asparaginase, at 2,150,531 to 2,151,526
_RS17595
RPSI07_RS17600: RPSI07_RS17600 - ferredoxin--NADP reductase, at 2,151,642 to 2,152,418
_RS17600
Position (kb)
2150
2151
2152 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2150.622 kb on + strand at 2150.622 kb on + strand at 2150.623 kb on - strand at 2150.851 kb on - strand, within RPSI07_RS17595 at 2150.851 kb on - strand, within RPSI07_RS17595 at 2151.009 kb on + strand, within RPSI07_RS17595 at 2151.009 kb on + strand, within RPSI07_RS17595 at 2151.010 kb on - strand, within RPSI07_RS17595 at 2151.010 kb on - strand, within RPSI07_RS17595 at 2151.565 kb on - strand at 2151.565 kb on - strand at 2151.643 kb on + strand at 2151.644 kb on - strand at 2152.515 kb on + strand at 2152.516 kb on - strand at 2152.516 kb on - strand at 2152.516 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 2,150,622 + -2.6 2,150,622 + -0.3 2,150,623 - -0.1 2,150,851 - RPSI07_RS17595 0.32 -2.0 2,150,851 - RPSI07_RS17595 0.32 +0.6 2,151,009 + RPSI07_RS17595 0.48 -0.2 2,151,009 + RPSI07_RS17595 0.48 -1.7 2,151,010 - RPSI07_RS17595 0.48 -1.3 2,151,010 - RPSI07_RS17595 0.48 -0.8 2,151,565 - +3.0 2,151,565 - -0.8 2,151,643 + -0.9 2,151,644 - -1.2 2,152,515 + +1.4 2,152,516 - +0.5 2,152,516 - +0.2 2,152,516 - +0.6
Or see this region's nucleotide sequence