Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS14730 and RPSI07_RS14735 are separated by 4 nucleotides RPSI07_RS14735 and RPSI07_RS14740 are separated by 14 nucleotides RPSI07_RS14740 and RPSI07_RS14745 are separated by 37 nucleotides
RPSI07_RS14730: RPSI07_RS14730 - tryptophan synthase subunit beta, at 1,480,666 to 1,481,859
_RS14730
RPSI07_RS14735: RPSI07_RS14735 - site-specific DNA-methyltransferase, at 1,481,864 to 1,482,676
_RS14735
RPSI07_RS14740: RPSI07_RS14740 - tryptophan synthase subunit alpha, at 1,482,691 to 1,483,488
_RS14740
RPSI07_RS14745: RPSI07_RS14745 - acetyl-CoA carboxylase carboxyltransferase subunit beta, at 1,483,526 to 1,484,398
_RS14745
Position (kb) 1481
1482
1483 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1480.894 kb on + strand, within RPSI07_RS14730 at 1480.894 kb on + strand, within RPSI07_RS14730 at 1480.895 kb on - strand, within RPSI07_RS14730 at 1481.072 kb on - strand, within RPSI07_RS14730 at 1481.399 kb on - strand, within RPSI07_RS14730 at 1481.404 kb on + strand, within RPSI07_RS14730 at 1481.645 kb on - strand, within RPSI07_RS14730 at 1481.984 kb on + strand, within RPSI07_RS14735 at 1481.984 kb on + strand, within RPSI07_RS14735 at 1482.021 kb on - strand, within RPSI07_RS14735 at 1482.021 kb on - strand, within RPSI07_RS14735 at 1482.086 kb on + strand, within RPSI07_RS14735 at 1482.257 kb on + strand, within RPSI07_RS14735 at 1482.257 kb on + strand, within RPSI07_RS14735 at 1482.690 kb on + strand at 1482.690 kb on + strand at 1483.207 kb on + strand, within RPSI07_RS14740 at 1483.216 kb on + strand, within RPSI07_RS14740
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 1,480,894 + RPSI07_RS14730 0.19 +2.4 1,480,894 + RPSI07_RS14730 0.19 +1.4 1,480,895 - RPSI07_RS14730 0.19 -0.2 1,481,072 - RPSI07_RS14730 0.34 +1.4 1,481,399 - RPSI07_RS14730 0.61 +1.4 1,481,404 + RPSI07_RS14730 0.62 +1.4 1,481,645 - RPSI07_RS14730 0.82 -0.3 1,481,984 + RPSI07_RS14735 0.15 -3.3 1,481,984 + RPSI07_RS14735 0.15 -1.9 1,482,021 - RPSI07_RS14735 0.19 -1.6 1,482,021 - RPSI07_RS14735 0.19 +2.4 1,482,086 + RPSI07_RS14735 0.27 -0.4 1,482,257 + RPSI07_RS14735 0.48 -0.2 1,482,257 + RPSI07_RS14735 0.48 -3.7 1,482,690 + +2.0 1,482,690 + +0.8 1,483,207 + RPSI07_RS14740 0.65 +0.4 1,483,216 + RPSI07_RS14740 0.66 -0.2
Or see this region's nucleotide sequence