Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS14725

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS14715 and RPSI07_RS14720 are separated by 85 nucleotidesRPSI07_RS14720 and RPSI07_RS14725 overlap by 4 nucleotidesRPSI07_RS14725 and RPSI07_RS14730 are separated by 94 nucleotides RPSI07_RS14715: RPSI07_RS14715 - peptidoglycan-binding protein, at 1,476,039 to 1,478,960 _RS14715 RPSI07_RS14720: RPSI07_RS14720 - tRNA pseudouridine(38-40) synthase TruA, at 1,479,046 to 1,479,870 _RS14720 RPSI07_RS14725: RPSI07_RS14725 - phosphoribosylanthranilate isomerase, at 1,479,867 to 1,480,571 _RS14725 RPSI07_RS14730: RPSI07_RS14730 - tryptophan synthase subunit beta, at 1,480,666 to 1,481,859 _RS14730 Position (kb) 1479 1480 1481Strain fitness (log2 ratio) -2 -1 0 1 2at 1479.373 kb on + strand, within RPSI07_RS14720at 1479.380 kb on - strand, within RPSI07_RS14720at 1479.714 kb on - strand, within RPSI07_RS14720at 1480.670 kb on - strandat 1480.804 kb on + strand, within RPSI07_RS14730at 1480.805 kb on - strand, within RPSI07_RS14730at 1480.850 kb on - strand, within RPSI07_RS14730at 1480.894 kb on + strand, within RPSI07_RS14730at 1480.894 kb on + strand, within RPSI07_RS14730at 1480.895 kb on - strand, within RPSI07_RS14730at 1481.072 kb on - strand, within RPSI07_RS14730at 1481.399 kb on - strand, within RPSI07_RS14730at 1481.404 kb on + strand, within RPSI07_RS14730

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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1,479,373 + RPSI07_RS14720 0.40 -1.8
1,479,380 - RPSI07_RS14720 0.40 +0.4
1,479,714 - RPSI07_RS14720 0.81 -0.9
1,480,670 - +2.0
1,480,804 + RPSI07_RS14730 0.12 +2.0
1,480,805 - RPSI07_RS14730 0.12 +2.4
1,480,850 - RPSI07_RS14730 0.15 +1.4
1,480,894 + RPSI07_RS14730 0.19 +2.4
1,480,894 + RPSI07_RS14730 0.19 +1.4
1,480,895 - RPSI07_RS14730 0.19 -0.2
1,481,072 - RPSI07_RS14730 0.34 +1.4
1,481,399 - RPSI07_RS14730 0.61 +1.4
1,481,404 + RPSI07_RS14730 0.62 +1.4

Or see this region's nucleotide sequence