Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS13465

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS13455 and RPSI07_RS13460 are separated by 34 nucleotidesRPSI07_RS13460 and RPSI07_RS13465 overlap by 10 nucleotidesRPSI07_RS13465 and RPSI07_RS13470 are separated by 85 nucleotidesRPSI07_RS13470 and RPSI07_RS13475 are separated by 82 nucleotides RPSI07_RS13455: RPSI07_RS13455 - DNA-dependent helicase, at 1,207,676 to 1,210,063 _RS13455 RPSI07_RS13460: RPSI07_RS13460 - hypothetical protein, at 1,210,098 to 1,210,895 _RS13460 RPSI07_RS13465: RPSI07_RS13465 - homoserine kinase, at 1,210,886 to 1,211,890 _RS13465 RPSI07_RS13470: RPSI07_RS13470 - membrane protein, at 1,211,976 to 1,212,404 _RS13470 RPSI07_RS13475: RPSI07_RS13475 - TIGR00730 family Rossman fold protein, at 1,212,487 to 1,213,449 _RS13475 Position (kb) 1210 1211 1212Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1210.156 kb on + strandat 1210.166 kb on - strandat 1210.178 kb on - strand, within RPSI07_RS13460at 1210.435 kb on + strand, within RPSI07_RS13460at 1210.435 kb on + strand, within RPSI07_RS13460at 1210.435 kb on + strand, within RPSI07_RS13460at 1210.436 kb on - strand, within RPSI07_RS13460at 1210.436 kb on - strand, within RPSI07_RS13460at 1210.465 kb on + strand, within RPSI07_RS13460at 1210.465 kb on + strand, within RPSI07_RS13460at 1210.465 kb on + strand, within RPSI07_RS13460at 1210.465 kb on + strand, within RPSI07_RS13460at 1210.580 kb on - strand, within RPSI07_RS13460at 1210.789 kb on + strand, within RPSI07_RS13460at 1210.861 kb on + strandat 1210.861 kb on + strandat 1210.861 kb on + strandat 1210.862 kb on - strandat 1210.907 kb on - strandat 1210.907 kb on - strandat 1212.440 kb on + strandat 1212.488 kb on + strandat 1212.488 kb on + strandat 1212.488 kb on + strandat 1212.495 kb on - strandat 1212.545 kb on + strandat 1212.555 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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1,210,156 + +0.4
1,210,166 - -0.4
1,210,178 - RPSI07_RS13460 0.10 -0.6
1,210,435 + RPSI07_RS13460 0.42 +2.2
1,210,435 + RPSI07_RS13460 0.42 +0.2
1,210,435 + RPSI07_RS13460 0.42 -1.0
1,210,436 - RPSI07_RS13460 0.42 -2.1
1,210,436 - RPSI07_RS13460 0.42 -0.7
1,210,465 + RPSI07_RS13460 0.46 +1.1
1,210,465 + RPSI07_RS13460 0.46 +1.5
1,210,465 + RPSI07_RS13460 0.46 +3.1
1,210,465 + RPSI07_RS13460 0.46 -0.6
1,210,580 - RPSI07_RS13460 0.60 +2.5
1,210,789 + RPSI07_RS13460 0.87 +0.2
1,210,861 + +1.0
1,210,861 + -0.6
1,210,861 + +0.7
1,210,862 - -0.1
1,210,907 - -3.7
1,210,907 - +2.5
1,212,440 + +1.3
1,212,488 + +1.5
1,212,488 + -0.3
1,212,488 + -0.4
1,212,495 - -2.2
1,212,545 + -1.0
1,212,555 - +0.1

Or see this region's nucleotide sequence