Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS06775 and RPSI07_RS06780 are separated by 53 nucleotides RPSI07_RS06780 and RPSI07_RS06785 are separated by 169 nucleotides RPSI07_RS06785 and RPSI07_RS06790 are separated by 23 nucleotides RPSI07_RS06790 and RPSI07_RS06795 are separated by 51 nucleotides RPSI07_RS06795 and RPSI07_RS06800 are separated by 74 nucleotides
RPSI07_RS06775: RPSI07_RS06775 - DUF488 domain-containing protein, at 1,815,825 to 1,816,223
_RS06775
RPSI07_RS06780: RPSI07_RS06780 - hypothetical protein, at 1,816,277 to 1,816,789
_RS06780
RPSI07_RS06785: RPSI07_RS06785 - DUF1059 domain-containing protein, at 1,816,959 to 1,817,177
_RS06785
RPSI07_RS06790: RPSI07_RS06790 - ATPase, at 1,817,201 to 1,817,743
_RS06790
RPSI07_RS06795: RPSI07_RS06795 - transcriptional regulator, at 1,817,795 to 1,818,166
_RS06795
RPSI07_RS06800: RPSI07_RS06800 - helix-turn-helix transcriptional regulator, at 1,818,241 to 1,818,891
_RS06800
Position (kb)
1817
1818 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1816.236 kb on + strand at 1816.237 kb on - strand at 1816.237 kb on - strand at 1816.277 kb on - strand at 1816.277 kb on - strand at 1816.277 kb on - strand at 1816.310 kb on + strand at 1816.310 kb on + strand at 1816.311 kb on - strand at 1816.311 kb on - strand at 1816.311 kb on - strand at 1816.311 kb on - strand at 1816.311 kb on - strand at 1816.430 kb on + strand, within RPSI07_RS06780 at 1816.430 kb on + strand, within RPSI07_RS06780 at 1816.430 kb on + strand, within RPSI07_RS06780 at 1816.431 kb on - strand, within RPSI07_RS06780 at 1816.457 kb on + strand, within RPSI07_RS06780 at 1816.457 kb on + strand, within RPSI07_RS06780 at 1816.458 kb on - strand, within RPSI07_RS06780 at 1816.652 kb on + strand, within RPSI07_RS06780 at 1816.652 kb on + strand, within RPSI07_RS06780 at 1816.653 kb on - strand, within RPSI07_RS06780 at 1816.683 kb on - strand, within RPSI07_RS06780 at 1816.683 kb on - strand, within RPSI07_RS06780 at 1816.683 kb on - strand, within RPSI07_RS06780 at 1816.697 kb on + strand, within RPSI07_RS06780 at 1816.855 kb on + strand at 1816.856 kb on - strand at 1816.893 kb on + strand at 1816.893 kb on + strand at 1816.893 kb on + strand at 1816.893 kb on + strand at 1816.894 kb on - strand at 1816.894 kb on - strand at 1816.894 kb on - strand at 1816.895 kb on + strand at 1816.905 kb on - strand at 1816.971 kb on + strand at 1816.971 kb on + strand at 1816.971 kb on + strand at 1816.972 kb on - strand at 1816.972 kb on - strand at 1816.974 kb on - strand at 1817.216 kb on - strand at 1817.216 kb on - strand at 1817.226 kb on + strand at 1817.226 kb on + strand at 1817.226 kb on + strand at 1817.227 kb on - strand at 1817.227 kb on - strand at 1817.362 kb on + strand, within RPSI07_RS06790 at 1817.362 kb on + strand, within RPSI07_RS06790 at 1817.452 kb on + strand, within RPSI07_RS06790 at 1817.452 kb on + strand, within RPSI07_RS06790 at 1817.452 kb on + strand, within RPSI07_RS06790 at 1817.452 kb on + strand, within RPSI07_RS06790 at 1817.452 kb on + strand, within RPSI07_RS06790 at 1817.452 kb on + strand, within RPSI07_RS06790 at 1817.452 kb on + strand, within RPSI07_RS06790 at 1817.452 kb on + strand, within RPSI07_RS06790 at 1817.453 kb on - strand, within RPSI07_RS06790 at 1817.453 kb on - strand, within RPSI07_RS06790 at 1817.453 kb on - strand, within RPSI07_RS06790 at 1817.453 kb on - strand, within RPSI07_RS06790 at 1817.453 kb on - strand, within RPSI07_RS06790 at 1817.454 kb on + strand, within RPSI07_RS06790 at 1817.454 kb on + strand, within RPSI07_RS06790 at 1817.454 kb on + strand, within RPSI07_RS06790 at 1817.454 kb on + strand, within RPSI07_RS06790 at 1817.454 kb on + strand, within RPSI07_RS06790 at 1817.455 kb on - strand, within RPSI07_RS06790 at 1817.455 kb on - strand, within RPSI07_RS06790 at 1817.455 kb on - strand, within RPSI07_RS06790 at 1817.455 kb on - strand, within RPSI07_RS06790 at 1817.478 kb on + strand, within RPSI07_RS06790 at 1817.478 kb on + strand, within RPSI07_RS06790 at 1817.658 kb on + strand, within RPSI07_RS06790 at 1817.658 kb on + strand, within RPSI07_RS06790 at 1817.659 kb on - strand, within RPSI07_RS06790 at 1817.659 kb on - strand, within RPSI07_RS06790 at 1817.659 kb on - strand, within RPSI07_RS06790 at 1817.659 kb on - strand, within RPSI07_RS06790 at 1817.659 kb on - strand, within RPSI07_RS06790 at 1817.666 kb on + strand, within RPSI07_RS06790 at 1817.667 kb on - strand, within RPSI07_RS06790 at 1817.733 kb on + strand at 1817.733 kb on + strand at 1817.733 kb on + strand at 1817.733 kb on + strand at 1817.733 kb on + strand at 1817.733 kb on + strand at 1817.733 kb on + strand at 1817.734 kb on - strand at 1817.734 kb on - strand at 1817.734 kb on - strand at 1817.734 kb on - strand at 1817.881 kb on + strand, within RPSI07_RS06795 at 1817.881 kb on + strand, within RPSI07_RS06795 at 1817.881 kb on + strand, within RPSI07_RS06795 at 1817.882 kb on - strand, within RPSI07_RS06795 at 1817.882 kb on - strand, within RPSI07_RS06795 at 1818.352 kb on - strand, within RPSI07_RS06800 at 1818.360 kb on + strand, within RPSI07_RS06800
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 1,816,236 + -0.5 1,816,237 - +1.7 1,816,237 - -1.0 1,816,277 - -0.7 1,816,277 - -1.7 1,816,277 - -1.8 1,816,310 + -1.9 1,816,310 + -1.0 1,816,311 - +1.3 1,816,311 - -3.0 1,816,311 - +0.7 1,816,311 - -0.1 1,816,311 - -0.9 1,816,430 + RPSI07_RS06780 0.30 +0.1 1,816,430 + RPSI07_RS06780 0.30 +0.5 1,816,430 + RPSI07_RS06780 0.30 -0.0 1,816,431 - RPSI07_RS06780 0.30 -2.3 1,816,457 + RPSI07_RS06780 0.35 -1.2 1,816,457 + RPSI07_RS06780 0.35 -1.0 1,816,458 - RPSI07_RS06780 0.35 -1.1 1,816,652 + RPSI07_RS06780 0.73 -1.2 1,816,652 + RPSI07_RS06780 0.73 +1.3 1,816,653 - RPSI07_RS06780 0.73 -0.5 1,816,683 - RPSI07_RS06780 0.79 +2.3 1,816,683 - RPSI07_RS06780 0.79 +0.0 1,816,683 - RPSI07_RS06780 0.79 -0.9 1,816,697 + RPSI07_RS06780 0.82 +0.6 1,816,855 + +0.1 1,816,856 - -1.5 1,816,893 + +1.3 1,816,893 + +1.3 1,816,893 + -0.6 1,816,893 + +0.3 1,816,894 - -1.3 1,816,894 - -0.3 1,816,894 - +2.6 1,816,895 + -1.1 1,816,905 - -0.6 1,816,971 + -1.4 1,816,971 + -1.4 1,816,971 + -0.7 1,816,972 - -1.2 1,816,972 - -1.1 1,816,974 - -0.6 1,817,216 - -0.4 1,817,216 - -2.3 1,817,226 + +1.9 1,817,226 + -0.0 1,817,226 + +0.0 1,817,227 - -0.3 1,817,227 - +0.1 1,817,362 + RPSI07_RS06790 0.30 +1.6 1,817,362 + RPSI07_RS06790 0.30 -3.0 1,817,452 + RPSI07_RS06790 0.46 -0.5 1,817,452 + RPSI07_RS06790 0.46 -0.2 1,817,452 + RPSI07_RS06790 0.46 -1.9 1,817,452 + RPSI07_RS06790 0.46 +1.3 1,817,452 + RPSI07_RS06790 0.46 +0.5 1,817,452 + RPSI07_RS06790 0.46 +1.3 1,817,452 + RPSI07_RS06790 0.46 -0.6 1,817,452 + RPSI07_RS06790 0.46 -0.8 1,817,453 - RPSI07_RS06790 0.46 +0.1 1,817,453 - RPSI07_RS06790 0.46 +0.6 1,817,453 - RPSI07_RS06790 0.46 +0.3 1,817,453 - RPSI07_RS06790 0.46 +0.8 1,817,453 - RPSI07_RS06790 0.46 -0.3 1,817,454 + RPSI07_RS06790 0.47 +0.3 1,817,454 + RPSI07_RS06790 0.47 -1.3 1,817,454 + RPSI07_RS06790 0.47 +1.3 1,817,454 + RPSI07_RS06790 0.47 +1.8 1,817,454 + RPSI07_RS06790 0.47 +0.3 1,817,455 - RPSI07_RS06790 0.47 +5.2 1,817,455 - RPSI07_RS06790 0.47 -0.5 1,817,455 - RPSI07_RS06790 0.47 -1.6 1,817,455 - RPSI07_RS06790 0.47 -0.8 1,817,478 + RPSI07_RS06790 0.51 +3.0 1,817,478 + RPSI07_RS06790 0.51 +3.6 1,817,658 + RPSI07_RS06790 0.84 -1.5 1,817,658 + RPSI07_RS06790 0.84 +0.9 1,817,659 - RPSI07_RS06790 0.84 +1.0 1,817,659 - RPSI07_RS06790 0.84 -0.6 1,817,659 - RPSI07_RS06790 0.84 +5.0 1,817,659 - RPSI07_RS06790 0.84 -1.3 1,817,659 - RPSI07_RS06790 0.84 -0.0 1,817,666 + RPSI07_RS06790 0.86 +1.3 1,817,667 - RPSI07_RS06790 0.86 -1.6 1,817,733 + +0.7 1,817,733 + +1.3 1,817,733 + +0.2 1,817,733 + +1.3 1,817,733 + -0.1 1,817,733 + +0.3 1,817,733 + -0.7 1,817,734 - -2.1 1,817,734 - -1.9 1,817,734 - +0.5 1,817,734 - -2.2 1,817,881 + RPSI07_RS06795 0.23 -1.0 1,817,881 + RPSI07_RS06795 0.23 -1.2 1,817,881 + RPSI07_RS06795 0.23 -0.5 1,817,882 - RPSI07_RS06795 0.23 -1.0 1,817,882 - RPSI07_RS06795 0.23 +1.3 1,818,352 - RPSI07_RS06800 0.17 +0.7 1,818,360 + RPSI07_RS06800 0.18 -0.1
Or see this region's nucleotide sequence