Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS23170 and RPSI07_RS23175 are separated by 140 nucleotides RPSI07_RS23175 and RPSI07_RS23180 are separated by 41 nucleotides RPSI07_RS23180 and RPSI07_RS23185 are separated by 12 nucleotides
RPSI07_RS23170: RPSI07_RS23170 - LysR family transcriptional regulator, at 3,344,411 to 3,345,337
_RS23170
RPSI07_RS23175: RPSI07_RS23175 - ornithine--oxo-acid transaminase, at 3,345,478 to 3,346,704
_RS23175
RPSI07_RS23180: RPSI07_RS23180 - arginase, at 3,346,746 to 3,347,675
_RS23180
RPSI07_RS23185: RPSI07_RS23185 - diguanylate cyclase, at 3,347,688 to 3,348,080
_RS23185
Position (kb)
3345
3346
3347 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3344.715 kb on + strand, within RPSI07_RS23170 at 3344.715 kb on + strand, within RPSI07_RS23170 at 3344.716 kb on - strand, within RPSI07_RS23170 at 3344.736 kb on + strand, within RPSI07_RS23170 at 3344.736 kb on + strand, within RPSI07_RS23170 at 3344.736 kb on + strand, within RPSI07_RS23170 at 3344.736 kb on + strand, within RPSI07_RS23170 at 3344.736 kb on + strand, within RPSI07_RS23170 at 3344.835 kb on + strand, within RPSI07_RS23170 at 3344.835 kb on + strand, within RPSI07_RS23170 at 3344.835 kb on + strand, within RPSI07_RS23170 at 3344.835 kb on + strand, within RPSI07_RS23170 at 3344.836 kb on - strand, within RPSI07_RS23170 at 3344.877 kb on + strand, within RPSI07_RS23170 at 3344.878 kb on - strand, within RPSI07_RS23170 at 3345.022 kb on - strand, within RPSI07_RS23170 at 3345.022 kb on - strand, within RPSI07_RS23170 at 3345.022 kb on - strand, within RPSI07_RS23170 at 3345.228 kb on + strand, within RPSI07_RS23170 at 3345.308 kb on - strand at 3345.308 kb on - strand at 3345.308 kb on - strand at 3345.308 kb on - strand at 3345.316 kb on - strand at 3345.316 kb on - strand at 3345.505 kb on + strand at 3345.505 kb on + strand at 3345.505 kb on + strand at 3345.505 kb on + strand at 3345.505 kb on + strand at 3345.505 kb on + strand at 3345.505 kb on + strand at 3345.506 kb on - strand at 3345.506 kb on - strand at 3345.506 kb on - strand at 3345.506 kb on - strand at 3345.506 kb on - strand at 3345.506 kb on - strand at 3345.506 kb on - strand at 3345.506 kb on - strand at 3345.523 kb on + strand at 3345.523 kb on + strand at 3345.539 kb on - strand at 3345.539 kb on - strand at 3345.607 kb on + strand, within RPSI07_RS23175 at 3345.608 kb on - strand, within RPSI07_RS23175 at 3345.628 kb on + strand, within RPSI07_RS23175 at 3345.629 kb on - strand, within RPSI07_RS23175 at 3345.952 kb on + strand, within RPSI07_RS23175 at 3345.959 kb on - strand, within RPSI07_RS23175 at 3346.105 kb on + strand, within RPSI07_RS23175 at 3346.589 kb on - strand at 3346.589 kb on - strand at 3346.710 kb on + strand at 3346.711 kb on - strand at 3346.711 kb on - strand at 3346.712 kb on + strand at 3346.879 kb on + strand, within RPSI07_RS23180 at 3346.880 kb on - strand, within RPSI07_RS23180 at 3346.880 kb on - strand, within RPSI07_RS23180 at 3346.882 kb on + strand, within RPSI07_RS23180 at 3346.882 kb on + strand, within RPSI07_RS23180 at 3346.883 kb on - strand, within RPSI07_RS23180 at 3346.883 kb on - strand, within RPSI07_RS23180 at 3346.883 kb on - strand, within RPSI07_RS23180 at 3346.883 kb on - strand, within RPSI07_RS23180 at 3346.883 kb on - strand, within RPSI07_RS23180 at 3347.132 kb on + strand, within RPSI07_RS23180 at 3347.277 kb on + strand, within RPSI07_RS23180 at 3347.277 kb on + strand, within RPSI07_RS23180 at 3347.278 kb on - strand, within RPSI07_RS23180 at 3347.562 kb on + strand, within RPSI07_RS23180 at 3347.563 kb on - strand, within RPSI07_RS23180 at 3347.563 kb on - strand, within RPSI07_RS23180
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 3,344,715 + RPSI07_RS23170 0.33 +0.6 3,344,715 + RPSI07_RS23170 0.33 -0.4 3,344,716 - RPSI07_RS23170 0.33 +0.2 3,344,736 + RPSI07_RS23170 0.35 -0.0 3,344,736 + RPSI07_RS23170 0.35 -0.4 3,344,736 + RPSI07_RS23170 0.35 -1.3 3,344,736 + RPSI07_RS23170 0.35 -0.6 3,344,736 + RPSI07_RS23170 0.35 +0.7 3,344,835 + RPSI07_RS23170 0.46 +1.0 3,344,835 + RPSI07_RS23170 0.46 -1.3 3,344,835 + RPSI07_RS23170 0.46 -1.4 3,344,835 + RPSI07_RS23170 0.46 -0.2 3,344,836 - RPSI07_RS23170 0.46 -1.1 3,344,877 + RPSI07_RS23170 0.50 -0.9 3,344,878 - RPSI07_RS23170 0.50 +0.5 3,345,022 - RPSI07_RS23170 0.66 -1.1 3,345,022 - RPSI07_RS23170 0.66 -0.2 3,345,022 - RPSI07_RS23170 0.66 -1.0 3,345,228 + RPSI07_RS23170 0.88 -1.2 3,345,308 - +0.0 3,345,308 - -0.2 3,345,308 - -0.3 3,345,308 - +1.1 3,345,316 - -0.0 3,345,316 - -1.2 3,345,505 + -1.3 3,345,505 + -0.5 3,345,505 + +3.9 3,345,505 + -2.7 3,345,505 + -2.1 3,345,505 + -0.7 3,345,505 + -0.3 3,345,506 - -1.1 3,345,506 - -0.6 3,345,506 - +0.2 3,345,506 - +1.3 3,345,506 - -0.9 3,345,506 - +1.0 3,345,506 - +1.0 3,345,506 - -0.6 3,345,523 + -1.1 3,345,523 + -1.6 3,345,539 - -1.4 3,345,539 - +0.4 3,345,607 + RPSI07_RS23175 0.11 -0.3 3,345,608 - RPSI07_RS23175 0.11 +1.0 3,345,628 + RPSI07_RS23175 0.12 +0.1 3,345,629 - RPSI07_RS23175 0.12 -1.9 3,345,952 + RPSI07_RS23175 0.39 -0.7 3,345,959 - RPSI07_RS23175 0.39 -0.6 3,346,105 + RPSI07_RS23175 0.51 -1.4 3,346,589 - +2.5 3,346,589 - -3.8 3,346,710 + -0.1 3,346,711 - -1.3 3,346,711 - -1.2 3,346,712 + +1.3 3,346,879 + RPSI07_RS23180 0.14 +0.4 3,346,880 - RPSI07_RS23180 0.14 -2.0 3,346,880 - RPSI07_RS23180 0.14 -3.6 3,346,882 + RPSI07_RS23180 0.15 -1.2 3,346,882 + RPSI07_RS23180 0.15 -1.1 3,346,883 - RPSI07_RS23180 0.15 -1.7 3,346,883 - RPSI07_RS23180 0.15 +1.0 3,346,883 - RPSI07_RS23180 0.15 -1.5 3,346,883 - RPSI07_RS23180 0.15 -0.6 3,346,883 - RPSI07_RS23180 0.15 +2.0 3,347,132 + RPSI07_RS23180 0.42 -0.9 3,347,277 + RPSI07_RS23180 0.57 +2.0 3,347,277 + RPSI07_RS23180 0.57 -0.8 3,347,278 - RPSI07_RS23180 0.57 -3.0 3,347,562 + RPSI07_RS23180 0.88 -1.5 3,347,563 - RPSI07_RS23180 0.88 +1.0 3,347,563 - RPSI07_RS23180 0.88 -0.1
Or see this region's nucleotide sequence