Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS19850 and RPSI07_RS19855 overlap by 4 nucleotides RPSI07_RS19855 and RPSI07_RS19860 are separated by 27 nucleotides
RPSI07_RS19850: RPSI07_RS19850 - glycosyltransferase family 1 protein, at 2,636,623 to 2,637,738
_RS19850
RPSI07_RS19855: RPSI07_RS19855 - capsular polysaccharide biosynthesis protein, at 2,637,735 to 2,638,925
_RS19855
RPSI07_RS19860: RPSI07_RS19860 - glycosyltransferase family 1 protein, at 2,638,953 to 2,640,110
_RS19860
Position (kb)
2637
2638
2639 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2636.738 kb on - strand, within RPSI07_RS19850 at 2636.894 kb on - strand, within RPSI07_RS19850 at 2637.178 kb on + strand, within RPSI07_RS19850 at 2637.178 kb on + strand, within RPSI07_RS19850 at 2637.178 kb on + strand, within RPSI07_RS19850 at 2637.178 kb on + strand, within RPSI07_RS19850 at 2637.178 kb on + strand, within RPSI07_RS19850 at 2637.179 kb on - strand, within RPSI07_RS19850 at 2637.179 kb on - strand, within RPSI07_RS19850 at 2637.179 kb on - strand, within RPSI07_RS19850 at 2637.180 kb on - strand, within RPSI07_RS19850 at 2637.259 kb on + strand, within RPSI07_RS19850 at 2637.260 kb on - strand, within RPSI07_RS19850 at 2637.382 kb on + strand, within RPSI07_RS19850 at 2637.421 kb on + strand, within RPSI07_RS19850 at 2637.421 kb on + strand, within RPSI07_RS19850 at 2637.421 kb on + strand, within RPSI07_RS19850 at 2637.422 kb on - strand, within RPSI07_RS19850 at 2637.422 kb on - strand, within RPSI07_RS19850 at 2637.422 kb on - strand, within RPSI07_RS19850 at 2637.422 kb on - strand, within RPSI07_RS19850 at 2637.446 kb on - strand, within RPSI07_RS19850 at 2637.457 kb on + strand, within RPSI07_RS19850 at 2637.458 kb on - strand, within RPSI07_RS19850 at 2637.458 kb on - strand, within RPSI07_RS19850 at 2637.458 kb on - strand, within RPSI07_RS19850 at 2637.458 kb on - strand, within RPSI07_RS19850 at 2637.458 kb on - strand, within RPSI07_RS19850 at 2637.458 kb on - strand, within RPSI07_RS19850 at 2637.709 kb on - strand at 2637.712 kb on + strand at 2637.825 kb on + strand at 2637.826 kb on - strand at 2637.826 kb on - strand at 2638.005 kb on + strand, within RPSI07_RS19855 at 2638.005 kb on + strand, within RPSI07_RS19855 at 2638.005 kb on + strand, within RPSI07_RS19855 at 2638.005 kb on + strand, within RPSI07_RS19855 at 2638.006 kb on - strand, within RPSI07_RS19855 at 2638.527 kb on + strand, within RPSI07_RS19855 at 2638.527 kb on + strand, within RPSI07_RS19855 at 2638.528 kb on - strand, within RPSI07_RS19855 at 2638.827 kb on + strand at 2638.827 kb on + strand at 2638.827 kb on + strand at 2638.827 kb on + strand at 2638.828 kb on - strand at 2638.828 kb on - strand at 2638.828 kb on - strand at 2638.890 kb on + strand at 2638.890 kb on + strand at 2638.891 kb on - strand at 2638.935 kb on - strand at 2638.952 kb on + strand at 2639.043 kb on + strand at 2639.043 kb on + strand at 2639.044 kb on - strand at 2639.044 kb on - strand at 2639.044 kb on - strand at 2639.361 kb on + strand, within RPSI07_RS19860 at 2639.361 kb on + strand, within RPSI07_RS19860 at 2639.574 kb on + strand, within RPSI07_RS19860 at 2639.574 kb on + strand, within RPSI07_RS19860
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,636,738 - RPSI07_RS19850 0.10 -0.1 2,636,894 - RPSI07_RS19850 0.24 +1.1 2,637,178 + RPSI07_RS19850 0.50 +0.4 2,637,178 + RPSI07_RS19850 0.50 -0.7 2,637,178 + RPSI07_RS19850 0.50 +1.0 2,637,178 + RPSI07_RS19850 0.50 -1.4 2,637,178 + RPSI07_RS19850 0.50 -0.4 2,637,179 - RPSI07_RS19850 0.50 -2.8 2,637,179 - RPSI07_RS19850 0.50 -0.2 2,637,179 - RPSI07_RS19850 0.50 +0.3 2,637,180 - RPSI07_RS19850 0.50 +0.5 2,637,259 + RPSI07_RS19850 0.57 -0.4 2,637,260 - RPSI07_RS19850 0.57 +0.1 2,637,382 + RPSI07_RS19850 0.68 +1.0 2,637,421 + RPSI07_RS19850 0.72 +2.0 2,637,421 + RPSI07_RS19850 0.72 -0.2 2,637,421 + RPSI07_RS19850 0.72 -0.1 2,637,422 - RPSI07_RS19850 0.72 +0.5 2,637,422 - RPSI07_RS19850 0.72 +3.0 2,637,422 - RPSI07_RS19850 0.72 -1.5 2,637,422 - RPSI07_RS19850 0.72 +1.0 2,637,446 - RPSI07_RS19850 0.74 +0.1 2,637,457 + RPSI07_RS19850 0.75 +0.2 2,637,458 - RPSI07_RS19850 0.75 -1.0 2,637,458 - RPSI07_RS19850 0.75 +1.7 2,637,458 - RPSI07_RS19850 0.75 -0.0 2,637,458 - RPSI07_RS19850 0.75 -1.6 2,637,458 - RPSI07_RS19850 0.75 +0.5 2,637,458 - RPSI07_RS19850 0.75 -2.5 2,637,709 - +1.2 2,637,712 + -1.0 2,637,825 + -1.1 2,637,826 - -1.2 2,637,826 - +0.5 2,638,005 + RPSI07_RS19855 0.23 -0.6 2,638,005 + RPSI07_RS19855 0.23 -1.9 2,638,005 + RPSI07_RS19855 0.23 +0.2 2,638,005 + RPSI07_RS19855 0.23 +1.3 2,638,006 - RPSI07_RS19855 0.23 +0.3 2,638,527 + RPSI07_RS19855 0.66 -0.5 2,638,527 + RPSI07_RS19855 0.66 -0.9 2,638,528 - RPSI07_RS19855 0.67 -0.7 2,638,827 + +3.0 2,638,827 + +0.1 2,638,827 + -0.1 2,638,827 + -0.2 2,638,828 - +0.2 2,638,828 - -0.8 2,638,828 - -0.6 2,638,890 + -0.6 2,638,890 + +0.1 2,638,891 - +0.5 2,638,935 - -0.3 2,638,952 + +1.8 2,639,043 + -0.0 2,639,043 + -0.5 2,639,044 - -1.2 2,639,044 - +0.7 2,639,044 - +0.7 2,639,361 + RPSI07_RS19860 0.35 +0.3 2,639,361 + RPSI07_RS19860 0.35 +0.1 2,639,574 + RPSI07_RS19860 0.54 +0.1 2,639,574 + RPSI07_RS19860 0.54 -1.5
Or see this region's nucleotide sequence