Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS19550

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS19540 and RPSI07_RS19545 are separated by 94 nucleotidesRPSI07_RS19545 and RPSI07_RS19550 are separated by 108 nucleotidesRPSI07_RS19550 and RPSI07_RS19555 are separated by 73 nucleotidesRPSI07_RS19555 and RPSI07_RS19560 are separated by 23 nucleotides RPSI07_RS19540: RPSI07_RS19540 - threonine-phosphate decarboxylase, at 2,566,050 to 2,566,991 _RS19540 RPSI07_RS19545: RPSI07_RS19545 - CoA pyrophosphatase, at 2,567,086 to 2,567,769 _RS19545 RPSI07_RS19550: RPSI07_RS19550 - 50S ribosomal protein L19, at 2,567,878 to 2,568,264 _RS19550 RPSI07_RS19555: RPSI07_RS19555 - tRNA (guanosine(37)-N1)-methyltransferase TrmD, at 2,568,338 to 2,569,126 _RS19555 RPSI07_RS19560: RPSI07_RS19560 - ribosome maturation factor RimM, at 2,569,150 to 2,569,791 _RS19560 Position (kb) 2567 2568 2569Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2566.918 kb on + strandat 2566.919 kb on - strandat 2566.919 kb on - strandat 2566.975 kb on - strandat 2567.044 kb on + strandat 2567.106 kb on - strandat 2567.106 kb on - strandat 2567.147 kb on + strandat 2567.147 kb on + strandat 2567.147 kb on + strandat 2567.150 kb on + strandat 2567.150 kb on + strandat 2567.183 kb on + strand, within RPSI07_RS19545at 2567.183 kb on + strand, within RPSI07_RS19545at 2567.183 kb on + strand, within RPSI07_RS19545at 2567.184 kb on - strand, within RPSI07_RS19545at 2567.184 kb on - strand, within RPSI07_RS19545at 2567.184 kb on - strand, within RPSI07_RS19545at 2567.316 kb on + strandat 2567.358 kb on + strand, within RPSI07_RS19545at 2567.363 kb on + strand, within RPSI07_RS19545at 2567.364 kb on - strand, within RPSI07_RS19545

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,566,918 + -1.6
2,566,919 - -2.3
2,566,919 - -1.1
2,566,975 - -2.3
2,567,044 + -1.4
2,567,106 - -0.7
2,567,106 - -1.1
2,567,147 + +3.3
2,567,147 + -3.3
2,567,147 + -0.1
2,567,150 + -0.4
2,567,150 + +0.2
2,567,183 + RPSI07_RS19545 0.14 +1.8
2,567,183 + RPSI07_RS19545 0.14 +0.4
2,567,183 + RPSI07_RS19545 0.14 -2.9
2,567,184 - RPSI07_RS19545 0.14 -1.8
2,567,184 - RPSI07_RS19545 0.14 +0.8
2,567,184 - RPSI07_RS19545 0.14 -1.1
2,567,316 + +3.7
2,567,358 + RPSI07_RS19545 0.40 -0.4
2,567,363 + RPSI07_RS19545 0.40 -0.6
2,567,364 - RPSI07_RS19545 0.41 +0.9

Or see this region's nucleotide sequence