Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS19540 and RPSI07_RS19545 are separated by 94 nucleotides RPSI07_RS19545 and RPSI07_RS19550 are separated by 108 nucleotides RPSI07_RS19550 and RPSI07_RS19555 are separated by 73 nucleotides RPSI07_RS19555 and RPSI07_RS19560 are separated by 23 nucleotides
RPSI07_RS19540: RPSI07_RS19540 - threonine-phosphate decarboxylase, at 2,566,050 to 2,566,991
_RS19540
RPSI07_RS19545: RPSI07_RS19545 - CoA pyrophosphatase, at 2,567,086 to 2,567,769
_RS19545
RPSI07_RS19550: RPSI07_RS19550 - 50S ribosomal protein L19, at 2,567,878 to 2,568,264
_RS19550
RPSI07_RS19555: RPSI07_RS19555 - tRNA (guanosine(37)-N1)-methyltransferase TrmD, at 2,568,338 to 2,569,126
_RS19555
RPSI07_RS19560: RPSI07_RS19560 - ribosome maturation factor RimM, at 2,569,150 to 2,569,791
_RS19560
Position (kb)
2567
2568
2569 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2566.918 kb on + strand at 2566.919 kb on - strand at 2566.919 kb on - strand at 2566.975 kb on - strand at 2567.044 kb on + strand at 2567.106 kb on - strand at 2567.106 kb on - strand at 2567.147 kb on + strand at 2567.147 kb on + strand at 2567.147 kb on + strand at 2567.150 kb on + strand at 2567.150 kb on + strand at 2567.183 kb on + strand, within RPSI07_RS19545 at 2567.183 kb on + strand, within RPSI07_RS19545 at 2567.183 kb on + strand, within RPSI07_RS19545 at 2567.184 kb on - strand, within RPSI07_RS19545 at 2567.184 kb on - strand, within RPSI07_RS19545 at 2567.184 kb on - strand, within RPSI07_RS19545 at 2567.316 kb on + strand at 2567.358 kb on + strand, within RPSI07_RS19545 at 2567.363 kb on + strand, within RPSI07_RS19545 at 2567.364 kb on - strand, within RPSI07_RS19545
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,566,918 + -1.6 2,566,919 - -2.3 2,566,919 - -1.1 2,566,975 - -2.3 2,567,044 + -1.4 2,567,106 - -0.7 2,567,106 - -1.1 2,567,147 + +3.3 2,567,147 + -3.3 2,567,147 + -0.1 2,567,150 + -0.4 2,567,150 + +0.2 2,567,183 + RPSI07_RS19545 0.14 +1.8 2,567,183 + RPSI07_RS19545 0.14 +0.4 2,567,183 + RPSI07_RS19545 0.14 -2.9 2,567,184 - RPSI07_RS19545 0.14 -1.8 2,567,184 - RPSI07_RS19545 0.14 +0.8 2,567,184 - RPSI07_RS19545 0.14 -1.1 2,567,316 + +3.7 2,567,358 + RPSI07_RS19545 0.40 -0.4 2,567,363 + RPSI07_RS19545 0.40 -0.6 2,567,364 - RPSI07_RS19545 0.41 +0.9
Or see this region's nucleotide sequence