Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS13885 and RPSI07_RS13890 are separated by 64 nucleotides RPSI07_RS13890 and RPSI07_RS13895 are separated by 111 nucleotides RPSI07_RS13895 and RPSI07_RS13900 are separated by 17 nucleotides RPSI07_RS13900 and RPSI07_RS13905 are separated by 289 nucleotides RPSI07_RS13905 and RPSI07_RS13910 are separated by 207 nucleotides
RPSI07_RS13885: RPSI07_RS13885 - hypothetical protein, at 1,299,415 to 1,299,810
_RS13885
RPSI07_RS13890: RPSI07_RS13890 - hypothetical protein, at 1,299,875 to 1,300,282
_RS13890
RPSI07_RS13895: RPSI07_RS13895 - hypothetical protein, at 1,300,394 to 1,300,699
_RS13895
RPSI07_RS13900: RPSI07_RS13900 - esterase, at 1,300,717 to 1,301,748
_RS13900
RPSI07_RS13905: RPSI07_RS13905 - peptidoglycan-binding protein LysM, at 1,302,038 to 1,302,520
_RS13905
RPSI07_RS13910: RPSI07_RS13910 - hypothetical protein, at 1,302,728 to 1,303,222
_RS13910
Position (kb)
1300
1301
1302 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1299.798 kb on + strand at 1299.808 kb on + strand at 1299.841 kb on + strand at 1299.842 kb on - strand at 1299.842 kb on - strand at 1299.842 kb on - strand at 1300.007 kb on + strand, within RPSI07_RS13890 at 1300.007 kb on + strand, within RPSI07_RS13890 at 1300.007 kb on + strand, within RPSI07_RS13890 at 1300.008 kb on - strand, within RPSI07_RS13890 at 1300.037 kb on + strand, within RPSI07_RS13890 at 1300.037 kb on + strand, within RPSI07_RS13890 at 1300.055 kb on + strand, within RPSI07_RS13890 at 1300.079 kb on + strand, within RPSI07_RS13890 at 1300.427 kb on + strand, within RPSI07_RS13895 at 1300.428 kb on - strand, within RPSI07_RS13895 at 1300.445 kb on + strand, within RPSI07_RS13895 at 1300.445 kb on + strand, within RPSI07_RS13895 at 1300.445 kb on + strand, within RPSI07_RS13895 at 1300.446 kb on - strand, within RPSI07_RS13895 at 1300.697 kb on + strand at 1300.806 kb on - strand at 1300.901 kb on + strand, within RPSI07_RS13900 at 1300.901 kb on + strand, within RPSI07_RS13900 at 1300.902 kb on - strand, within RPSI07_RS13900 at 1300.902 kb on - strand, within RPSI07_RS13900 at 1301.348 kb on + strand, within RPSI07_RS13900 at 1301.348 kb on + strand, within RPSI07_RS13900 at 1301.348 kb on + strand, within RPSI07_RS13900 at 1301.348 kb on + strand, within RPSI07_RS13900 at 1301.349 kb on - strand, within RPSI07_RS13900 at 1301.349 kb on - strand, within RPSI07_RS13900 at 1301.349 kb on - strand, within RPSI07_RS13900 at 1301.349 kb on - strand, within RPSI07_RS13900 at 1301.349 kb on - strand, within RPSI07_RS13900 at 1301.443 kb on - strand, within RPSI07_RS13900 at 1301.443 kb on - strand, within RPSI07_RS13900 at 1301.443 kb on - strand, within RPSI07_RS13900 at 1301.531 kb on + strand, within RPSI07_RS13900 at 1301.531 kb on + strand, within RPSI07_RS13900 at 1301.531 kb on + strand, within RPSI07_RS13900 at 1301.532 kb on - strand, within RPSI07_RS13900 at 1301.532 kb on - strand, within RPSI07_RS13900 at 1301.541 kb on - strand, within RPSI07_RS13900 at 1301.582 kb on + strand, within RPSI07_RS13900 at 1301.583 kb on - strand, within RPSI07_RS13900 at 1301.583 kb on - strand, within RPSI07_RS13900 at 1301.754 kb on + strand at 1301.755 kb on - strand at 1301.755 kb on - strand at 1301.801 kb on + strand at 1301.801 kb on + strand at 1301.801 kb on + strand at 1301.802 kb on - strand at 1301.966 kb on - strand at 1302.173 kb on + strand, within RPSI07_RS13905 at 1302.173 kb on + strand, within RPSI07_RS13905 at 1302.173 kb on + strand, within RPSI07_RS13905 at 1302.174 kb on - strand, within RPSI07_RS13905 at 1302.174 kb on - strand, within RPSI07_RS13905 at 1302.174 kb on - strand, within RPSI07_RS13905 at 1302.368 kb on - strand, within RPSI07_RS13905 at 1302.414 kb on - strand, within RPSI07_RS13905 at 1302.414 kb on - strand, within RPSI07_RS13905 at 1302.485 kb on + strand at 1302.486 kb on - strand at 1302.518 kb on + strand at 1302.518 kb on + strand at 1302.518 kb on + strand at 1302.518 kb on + strand at 1302.518 kb on + strand at 1302.518 kb on + strand at 1302.518 kb on + strand at 1302.519 kb on - strand at 1302.519 kb on - strand at 1302.519 kb on - strand at 1302.519 kb on - strand at 1302.519 kb on - strand at 1302.649 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,299,798 + +1.3 1,299,808 + +0.2 1,299,841 + +1.2 1,299,842 - +1.2 1,299,842 - +0.1 1,299,842 - +1.8 1,300,007 + RPSI07_RS13890 0.32 +0.6 1,300,007 + RPSI07_RS13890 0.32 -1.7 1,300,007 + RPSI07_RS13890 0.32 -1.3 1,300,008 - RPSI07_RS13890 0.33 +1.0 1,300,037 + RPSI07_RS13890 0.40 -1.9 1,300,037 + RPSI07_RS13890 0.40 -1.3 1,300,055 + RPSI07_RS13890 0.44 -0.1 1,300,079 + RPSI07_RS13890 0.50 +0.3 1,300,427 + RPSI07_RS13895 0.11 -0.6 1,300,428 - RPSI07_RS13895 0.11 -0.3 1,300,445 + RPSI07_RS13895 0.17 +1.0 1,300,445 + RPSI07_RS13895 0.17 +0.8 1,300,445 + RPSI07_RS13895 0.17 -0.3 1,300,446 - RPSI07_RS13895 0.17 +2.0 1,300,697 + -0.1 1,300,806 - -0.4 1,300,901 + RPSI07_RS13900 0.18 +3.2 1,300,901 + RPSI07_RS13900 0.18 +0.0 1,300,902 - RPSI07_RS13900 0.18 -1.0 1,300,902 - RPSI07_RS13900 0.18 -1.4 1,301,348 + RPSI07_RS13900 0.61 +0.6 1,301,348 + RPSI07_RS13900 0.61 +0.7 1,301,348 + RPSI07_RS13900 0.61 -0.1 1,301,348 + RPSI07_RS13900 0.61 +0.0 1,301,349 - RPSI07_RS13900 0.61 -2.2 1,301,349 - RPSI07_RS13900 0.61 -0.6 1,301,349 - RPSI07_RS13900 0.61 +2.0 1,301,349 - RPSI07_RS13900 0.61 +3.3 1,301,349 - RPSI07_RS13900 0.61 -0.8 1,301,443 - RPSI07_RS13900 0.70 -0.8 1,301,443 - RPSI07_RS13900 0.70 -2.2 1,301,443 - RPSI07_RS13900 0.70 +1.0 1,301,531 + RPSI07_RS13900 0.79 +0.1 1,301,531 + RPSI07_RS13900 0.79 -0.3 1,301,531 + RPSI07_RS13900 0.79 +0.0 1,301,532 - RPSI07_RS13900 0.79 +0.5 1,301,532 - RPSI07_RS13900 0.79 +0.8 1,301,541 - RPSI07_RS13900 0.80 -0.7 1,301,582 + RPSI07_RS13900 0.84 +0.8 1,301,583 - RPSI07_RS13900 0.84 +0.7 1,301,583 - RPSI07_RS13900 0.84 -2.5 1,301,754 + +0.0 1,301,755 - -0.1 1,301,755 - +2.3 1,301,801 + -1.6 1,301,801 + -1.5 1,301,801 + -1.0 1,301,802 - -1.1 1,301,966 - +1.0 1,302,173 + RPSI07_RS13905 0.28 -0.1 1,302,173 + RPSI07_RS13905 0.28 +0.3 1,302,173 + RPSI07_RS13905 0.28 -0.3 1,302,174 - RPSI07_RS13905 0.28 +0.0 1,302,174 - RPSI07_RS13905 0.28 +0.9 1,302,174 - RPSI07_RS13905 0.28 +0.8 1,302,368 - RPSI07_RS13905 0.68 +0.1 1,302,414 - RPSI07_RS13905 0.78 +0.1 1,302,414 - RPSI07_RS13905 0.78 +1.1 1,302,485 + -1.2 1,302,486 - -0.6 1,302,518 + -0.1 1,302,518 + -0.5 1,302,518 + -0.6 1,302,518 + +0.7 1,302,518 + +0.9 1,302,518 + +0.9 1,302,518 + -1.1 1,302,519 - +1.0 1,302,519 - -1.3 1,302,519 - -0.3 1,302,519 - -1.0 1,302,519 - +0.5 1,302,649 - +0.1
Or see this region's nucleotide sequence