Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS13615 and RPSI07_RS13620 are separated by 5 nucleotides RPSI07_RS13620 and RPSI07_RS13625 are separated by 39 nucleotides
RPSI07_RS13615: RPSI07_RS13615 - O-antigen ligase family protein, at 1,247,147 to 1,248,454
_RS13615
RPSI07_RS13620: RPSI07_RS13620 - glycosyl transferase, at 1,248,460 to 1,249,380
_RS13620
RPSI07_RS13625: RPSI07_RS13625 - glycosyltransferase family 2 protein, at 1,249,420 to 1,250,190
_RS13625
Position (kb)
1248
1249
1250 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1247.901 kb on - strand, within RPSI07_RS13615 at 1248.008 kb on + strand, within RPSI07_RS13615 at 1248.009 kb on - strand, within RPSI07_RS13615 at 1248.164 kb on + strand, within RPSI07_RS13615 at 1248.468 kb on + strand at 1248.498 kb on + strand at 1248.718 kb on + strand, within RPSI07_RS13620 at 1248.718 kb on + strand, within RPSI07_RS13620 at 1248.718 kb on + strand at 1248.718 kb on + strand, within RPSI07_RS13620 at 1248.718 kb on + strand, within RPSI07_RS13620 at 1248.719 kb on - strand, within RPSI07_RS13620 at 1248.719 kb on - strand, within RPSI07_RS13620 at 1248.719 kb on - strand, within RPSI07_RS13620 at 1248.785 kb on - strand, within RPSI07_RS13620 at 1249.091 kb on - strand, within RPSI07_RS13620 at 1249.193 kb on - strand, within RPSI07_RS13620 at 1249.196 kb on + strand, within RPSI07_RS13620 at 1249.279 kb on + strand, within RPSI07_RS13620 at 1249.294 kb on + strand at 1249.476 kb on - strand at 1249.644 kb on - strand, within RPSI07_RS13625 at 1249.682 kb on + strand, within RPSI07_RS13625 at 1249.682 kb on + strand, within RPSI07_RS13625 at 1249.682 kb on + strand, within RPSI07_RS13625 at 1249.682 kb on + strand, within RPSI07_RS13625 at 1249.682 kb on + strand, within RPSI07_RS13625 at 1249.683 kb on - strand, within RPSI07_RS13625 at 1249.683 kb on - strand, within RPSI07_RS13625 at 1249.683 kb on - strand, within RPSI07_RS13625 at 1249.799 kb on + strand, within RPSI07_RS13625 at 1249.866 kb on - strand, within RPSI07_RS13625 at 1250.278 kb on - strand at 1250.278 kb on - strand at 1250.326 kb on + strand at 1250.372 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,247,901 - RPSI07_RS13615 0.58 -1.8 1,248,008 + RPSI07_RS13615 0.66 -3.4 1,248,009 - RPSI07_RS13615 0.66 -3.2 1,248,164 + RPSI07_RS13615 0.78 +1.0 1,248,468 + -3.2 1,248,498 + +1.7 1,248,718 + RPSI07_RS13620 0.28 -1.3 1,248,718 + RPSI07_RS13620 0.28 -1.0 1,248,718 + +1.0 1,248,718 + RPSI07_RS13620 0.28 -2.5 1,248,718 + RPSI07_RS13620 0.28 -4.0 1,248,719 - RPSI07_RS13620 0.28 +1.0 1,248,719 - RPSI07_RS13620 0.28 -1.6 1,248,719 - RPSI07_RS13620 0.28 -2.8 1,248,785 - RPSI07_RS13620 0.35 -2.7 1,249,091 - RPSI07_RS13620 0.69 +0.0 1,249,193 - RPSI07_RS13620 0.80 +0.0 1,249,196 + RPSI07_RS13620 0.80 -3.1 1,249,279 + RPSI07_RS13620 0.89 -1.9 1,249,294 + -1.3 1,249,476 - -2.3 1,249,644 - RPSI07_RS13625 0.29 -3.3 1,249,682 + RPSI07_RS13625 0.34 -4.3 1,249,682 + RPSI07_RS13625 0.34 +0.0 1,249,682 + RPSI07_RS13625 0.34 -1.8 1,249,682 + RPSI07_RS13625 0.34 -3.1 1,249,682 + RPSI07_RS13625 0.34 -1.0 1,249,683 - RPSI07_RS13625 0.34 -3.7 1,249,683 - RPSI07_RS13625 0.34 -5.2 1,249,683 - RPSI07_RS13625 0.34 -3.1 1,249,799 + RPSI07_RS13625 0.49 -4.1 1,249,866 - RPSI07_RS13625 0.58 +1.0 1,250,278 - -0.7 1,250,278 - -1.5 1,250,326 + -2.2 1,250,372 - -5.9
Or see this region's nucleotide sequence