Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS13620

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS13615 and RPSI07_RS13620 are separated by 5 nucleotidesRPSI07_RS13620 and RPSI07_RS13625 are separated by 39 nucleotides RPSI07_RS13615: RPSI07_RS13615 - O-antigen ligase family protein, at 1,247,147 to 1,248,454 _RS13615 RPSI07_RS13620: RPSI07_RS13620 - glycosyl transferase, at 1,248,460 to 1,249,380 _RS13620 RPSI07_RS13625: RPSI07_RS13625 - glycosyltransferase family 2 protein, at 1,249,420 to 1,250,190 _RS13625 Position (kb) 1248 1249 1250Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 1247.901 kb on - strand, within RPSI07_RS13615at 1248.008 kb on + strand, within RPSI07_RS13615at 1248.009 kb on - strand, within RPSI07_RS13615at 1248.164 kb on + strand, within RPSI07_RS13615at 1248.468 kb on + strandat 1248.498 kb on + strandat 1248.718 kb on + strand, within RPSI07_RS13620at 1248.718 kb on + strand, within RPSI07_RS13620at 1248.718 kb on + strandat 1248.718 kb on + strand, within RPSI07_RS13620at 1248.718 kb on + strand, within RPSI07_RS13620at 1248.719 kb on - strand, within RPSI07_RS13620at 1248.719 kb on - strand, within RPSI07_RS13620at 1248.719 kb on - strand, within RPSI07_RS13620at 1248.785 kb on - strand, within RPSI07_RS13620at 1249.091 kb on - strand, within RPSI07_RS13620at 1249.193 kb on - strand, within RPSI07_RS13620at 1249.196 kb on + strand, within RPSI07_RS13620at 1249.279 kb on + strand, within RPSI07_RS13620at 1249.294 kb on + strandat 1249.476 kb on - strandat 1249.644 kb on - strand, within RPSI07_RS13625at 1249.682 kb on + strand, within RPSI07_RS13625at 1249.682 kb on + strand, within RPSI07_RS13625at 1249.682 kb on + strand, within RPSI07_RS13625at 1249.682 kb on + strand, within RPSI07_RS13625at 1249.682 kb on + strand, within RPSI07_RS13625at 1249.683 kb on - strand, within RPSI07_RS13625at 1249.683 kb on - strand, within RPSI07_RS13625at 1249.683 kb on - strand, within RPSI07_RS13625at 1249.799 kb on + strand, within RPSI07_RS13625at 1249.866 kb on - strand, within RPSI07_RS13625at 1250.278 kb on - strandat 1250.278 kb on - strandat 1250.326 kb on + strandat 1250.372 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,247,901 - RPSI07_RS13615 0.58 -1.8
1,248,008 + RPSI07_RS13615 0.66 -3.4
1,248,009 - RPSI07_RS13615 0.66 -3.2
1,248,164 + RPSI07_RS13615 0.78 +1.0
1,248,468 + -3.2
1,248,498 + +1.7
1,248,718 + RPSI07_RS13620 0.28 -1.3
1,248,718 + RPSI07_RS13620 0.28 -1.0
1,248,718 + +1.0
1,248,718 + RPSI07_RS13620 0.28 -2.5
1,248,718 + RPSI07_RS13620 0.28 -4.0
1,248,719 - RPSI07_RS13620 0.28 +1.0
1,248,719 - RPSI07_RS13620 0.28 -1.6
1,248,719 - RPSI07_RS13620 0.28 -2.8
1,248,785 - RPSI07_RS13620 0.35 -2.7
1,249,091 - RPSI07_RS13620 0.69 +0.0
1,249,193 - RPSI07_RS13620 0.80 +0.0
1,249,196 + RPSI07_RS13620 0.80 -3.1
1,249,279 + RPSI07_RS13620 0.89 -1.9
1,249,294 + -1.3
1,249,476 - -2.3
1,249,644 - RPSI07_RS13625 0.29 -3.3
1,249,682 + RPSI07_RS13625 0.34 -4.3
1,249,682 + RPSI07_RS13625 0.34 +0.0
1,249,682 + RPSI07_RS13625 0.34 -1.8
1,249,682 + RPSI07_RS13625 0.34 -3.1
1,249,682 + RPSI07_RS13625 0.34 -1.0
1,249,683 - RPSI07_RS13625 0.34 -3.7
1,249,683 - RPSI07_RS13625 0.34 -5.2
1,249,683 - RPSI07_RS13625 0.34 -3.1
1,249,799 + RPSI07_RS13625 0.49 -4.1
1,249,866 - RPSI07_RS13625 0.58 +1.0
1,250,278 - -0.7
1,250,278 - -1.5
1,250,326 + -2.2
1,250,372 - -5.9

Or see this region's nucleotide sequence