Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS13115 and RPSI07_RS13120 are separated by 33 nucleotides RPSI07_RS13120 and RPSI07_RS13125 are separated by 29 nucleotides RPSI07_RS13125 and RPSI07_RS13130 are separated by 145 nucleotides
RPSI07_RS13115: RPSI07_RS13115 - heme biosynthesis protein HemY, at 1,130,444 to 1,131,637
_RS13115
RPSI07_RS13120: RPSI07_RS13120 - cytochrome B oxidoreductase, at 1,131,671 to 1,132,198
_RS13120
RPSI07_RS13125: RPSI07_RS13125 - hypothetical protein, at 1,132,228 to 1,132,494
_RS13125
RPSI07_RS13130: RPSI07_RS13130 - MFS transporter, at 1,132,640 to 1,133,968
_RS13130
Position (kb)
1132
1133 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1131.244 kb on + strand, within RPSI07_RS13115 at 1131.245 kb on + strand, within RPSI07_RS13115 at 1131.429 kb on - strand, within RPSI07_RS13115 at 1131.503 kb on + strand, within RPSI07_RS13115 at 1131.504 kb on - strand, within RPSI07_RS13115 at 1131.504 kb on - strand, within RPSI07_RS13115 at 1131.504 kb on - strand, within RPSI07_RS13115 at 1131.504 kb on - strand, within RPSI07_RS13115 at 1131.504 kb on - strand, within RPSI07_RS13115 at 1131.566 kb on + strand at 1131.566 kb on + strand at 1131.566 kb on + strand at 1131.567 kb on - strand at 1131.567 kb on - strand at 1131.567 kb on - strand at 1131.567 kb on - strand at 1131.567 kb on - strand at 1131.567 kb on - strand at 1131.635 kb on + strand at 1131.636 kb on - strand at 1131.636 kb on - strand at 1131.951 kb on - strand, within RPSI07_RS13120 at 1131.964 kb on - strand, within RPSI07_RS13120 at 1132.068 kb on + strand, within RPSI07_RS13120 at 1132.107 kb on + strand, within RPSI07_RS13120 at 1132.230 kb on - strand at 1132.230 kb on - strand at 1132.274 kb on + strand, within RPSI07_RS13125 at 1132.274 kb on + strand, within RPSI07_RS13125 at 1132.275 kb on - strand, within RPSI07_RS13125 at 1132.276 kb on + strand, within RPSI07_RS13125 at 1132.278 kb on - strand, within RPSI07_RS13125 at 1132.278 kb on - strand, within RPSI07_RS13125 at 1132.313 kb on + strand, within RPSI07_RS13125 at 1132.313 kb on + strand, within RPSI07_RS13125 at 1132.313 kb on + strand, within RPSI07_RS13125 at 1132.314 kb on - strand, within RPSI07_RS13125 at 1132.314 kb on - strand, within RPSI07_RS13125 at 1132.314 kb on - strand, within RPSI07_RS13125 at 1132.314 kb on - strand, within RPSI07_RS13125 at 1132.314 kb on - strand, within RPSI07_RS13125 at 1132.314 kb on - strand, within RPSI07_RS13125 at 1132.314 kb on - strand, within RPSI07_RS13125 at 1132.314 kb on - strand, within RPSI07_RS13125 at 1132.314 kb on - strand, within RPSI07_RS13125 at 1132.314 kb on - strand, within RPSI07_RS13125 at 1132.322 kb on + strand, within RPSI07_RS13125 at 1132.358 kb on + strand, within RPSI07_RS13125 at 1132.358 kb on + strand, within RPSI07_RS13125 at 1132.358 kb on + strand, within RPSI07_RS13125 at 1132.528 kb on + strand at 1132.528 kb on + strand at 1132.529 kb on - strand at 1132.529 kb on - strand at 1132.582 kb on - strand at 1132.713 kb on + strand at 1132.713 kb on + strand at 1132.765 kb on - strand at 1133.172 kb on + strand, within RPSI07_RS13130 at 1133.173 kb on - strand, within RPSI07_RS13130 at 1133.173 kb on - strand, within RPSI07_RS13130 at 1133.173 kb on - strand, within RPSI07_RS13130 at 1133.325 kb on + strand, within RPSI07_RS13130 at 1133.326 kb on - strand, within RPSI07_RS13130
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,131,244 + RPSI07_RS13115 0.67 -2.1 1,131,245 + RPSI07_RS13115 0.67 -2.4 1,131,429 - RPSI07_RS13115 0.82 -2.7 1,131,503 + RPSI07_RS13115 0.89 -2.5 1,131,504 - RPSI07_RS13115 0.89 -2.4 1,131,504 - RPSI07_RS13115 0.89 -1.1 1,131,504 - RPSI07_RS13115 0.89 -2.9 1,131,504 - RPSI07_RS13115 0.89 -2.4 1,131,504 - RPSI07_RS13115 0.89 -2.7 1,131,566 + -1.8 1,131,566 + -1.2 1,131,566 + -2.0 1,131,567 - -2.5 1,131,567 - -2.5 1,131,567 - -1.3 1,131,567 - -1.4 1,131,567 - -1.9 1,131,567 - -2.4 1,131,635 + +0.9 1,131,636 - -1.8 1,131,636 - +0.5 1,131,951 - RPSI07_RS13120 0.53 +1.0 1,131,964 - RPSI07_RS13120 0.55 -0.0 1,132,068 + RPSI07_RS13120 0.75 +1.0 1,132,107 + RPSI07_RS13120 0.83 +1.2 1,132,230 - +0.3 1,132,230 - +2.0 1,132,274 + RPSI07_RS13125 0.17 +0.6 1,132,274 + RPSI07_RS13125 0.17 +2.4 1,132,275 - RPSI07_RS13125 0.18 -0.4 1,132,276 + RPSI07_RS13125 0.18 +2.1 1,132,278 - RPSI07_RS13125 0.19 -0.2 1,132,278 - RPSI07_RS13125 0.19 -0.4 1,132,313 + RPSI07_RS13125 0.32 +2.8 1,132,313 + RPSI07_RS13125 0.32 +0.7 1,132,313 + RPSI07_RS13125 0.32 +0.5 1,132,314 - RPSI07_RS13125 0.32 -0.6 1,132,314 - RPSI07_RS13125 0.32 +0.4 1,132,314 - RPSI07_RS13125 0.32 -2.0 1,132,314 - RPSI07_RS13125 0.32 +0.4 1,132,314 - RPSI07_RS13125 0.32 +1.0 1,132,314 - RPSI07_RS13125 0.32 -1.6 1,132,314 - RPSI07_RS13125 0.32 -0.2 1,132,314 - RPSI07_RS13125 0.32 -1.0 1,132,314 - RPSI07_RS13125 0.32 +1.3 1,132,314 - RPSI07_RS13125 0.32 -0.6 1,132,322 + RPSI07_RS13125 0.35 +1.4 1,132,358 + RPSI07_RS13125 0.49 -0.5 1,132,358 + RPSI07_RS13125 0.49 +1.0 1,132,358 + RPSI07_RS13125 0.49 +0.8 1,132,528 + -0.0 1,132,528 + -1.0 1,132,529 - -0.6 1,132,529 - -0.4 1,132,582 - +0.7 1,132,713 + +0.2 1,132,713 + +0.7 1,132,765 - +1.1 1,133,172 + RPSI07_RS13130 0.40 +0.7 1,133,173 - RPSI07_RS13130 0.40 +1.4 1,133,173 - RPSI07_RS13130 0.40 -0.2 1,133,173 - RPSI07_RS13130 0.40 -0.3 1,133,325 + RPSI07_RS13130 0.52 +0.5 1,133,326 - RPSI07_RS13130 0.52 +0.6
Or see this region's nucleotide sequence