Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS11400 and RPSI07_RS11405 are separated by 13 nucleotides RPSI07_RS11405 and RPSI07_RS11410 overlap by 20 nucleotides RPSI07_RS11410 and RPSI07_RS11415 overlap by 4 nucleotides RPSI07_RS11415 and RPSI07_RS11420 overlap by 4 nucleotides
RPSI07_RS11400: RPSI07_RS11400 - L-arabinose ABC transporter permease AraH, at 754,193 to 755,200
_RS11400
RPSI07_RS11405: RPSI07_RS11405 - SMP-30/gluconolactonase/LRE family protein, at 755,214 to 756,125
_RS11405
RPSI07_RS11410: RPSI07_RS11410 - aldose 1-epimerase, at 756,106 to 756,996
_RS11410
RPSI07_RS11415: RPSI07_RS11415 - short-chain dehydrogenase, at 756,993 to 757,790
_RS11415
RPSI07_RS11420: RPSI07_RS11420 - 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, at 757,787 to 758,428
_RS11420
Position (kb) 756
757 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 755.107 kb on + strand at 755.120 kb on + strand at 755.120 kb on + strand at 755.121 kb on - strand at 755.121 kb on - strand at 755.126 kb on + strand at 755.127 kb on - strand at 755.544 kb on + strand, within RPSI07_RS11405 at 755.544 kb on + strand, within RPSI07_RS11405 at 755.544 kb on + strand, within RPSI07_RS11405 at 755.545 kb on - strand, within RPSI07_RS11405 at 755.545 kb on - strand, within RPSI07_RS11405 at 755.545 kb on - strand, within RPSI07_RS11405 at 755.545 kb on - strand, within RPSI07_RS11405 at 755.596 kb on - strand, within RPSI07_RS11405 at 755.691 kb on + strand, within RPSI07_RS11405 at 755.691 kb on + strand, within RPSI07_RS11405 at 755.691 kb on + strand, within RPSI07_RS11405 at 755.691 kb on + strand, within RPSI07_RS11405 at 755.692 kb on - strand, within RPSI07_RS11405 at 755.692 kb on - strand, within RPSI07_RS11405 at 755.692 kb on - strand, within RPSI07_RS11405 at 755.692 kb on - strand, within RPSI07_RS11405 at 755.692 kb on - strand, within RPSI07_RS11405 at 755.692 kb on - strand, within RPSI07_RS11405 at 755.734 kb on - strand, within RPSI07_RS11405 at 755.922 kb on + strand, within RPSI07_RS11405 at 755.979 kb on - strand, within RPSI07_RS11405 at 755.986 kb on - strand, within RPSI07_RS11405 at 755.986 kb on - strand, within RPSI07_RS11405 at 756.068 kb on + strand at 756.069 kb on - strand at 756.114 kb on - strand at 756.473 kb on - strand, within RPSI07_RS11410 at 756.818 kb on - strand, within RPSI07_RS11410 at 756.827 kb on - strand, within RPSI07_RS11410 at 756.827 kb on - strand, within RPSI07_RS11410 at 756.827 kb on - strand, within RPSI07_RS11410 at 756.827 kb on - strand, within RPSI07_RS11410 at 756.827 kb on - strand, within RPSI07_RS11410 at 756.827 kb on - strand, within RPSI07_RS11410 at 757.207 kb on + strand, within RPSI07_RS11415 at 757.533 kb on + strand, within RPSI07_RS11415 at 757.696 kb on - strand, within RPSI07_RS11415 at 757.780 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 755,107 + -0.0 755,120 + +1.0 755,120 + +1.9 755,121 - -1.2 755,121 - -0.0 755,126 + +1.0 755,127 - -0.7 755,544 + RPSI07_RS11405 0.36 -0.2 755,544 + RPSI07_RS11405 0.36 -0.7 755,544 + RPSI07_RS11405 0.36 +0.4 755,545 - RPSI07_RS11405 0.36 +0.7 755,545 - RPSI07_RS11405 0.36 -1.2 755,545 - RPSI07_RS11405 0.36 +0.5 755,545 - RPSI07_RS11405 0.36 -0.3 755,596 - RPSI07_RS11405 0.42 -0.0 755,691 + RPSI07_RS11405 0.52 +0.2 755,691 + RPSI07_RS11405 0.52 -1.7 755,691 + RPSI07_RS11405 0.52 +0.4 755,691 + RPSI07_RS11405 0.52 -1.3 755,692 - RPSI07_RS11405 0.52 +3.0 755,692 - RPSI07_RS11405 0.52 -0.4 755,692 - RPSI07_RS11405 0.52 -1.6 755,692 - RPSI07_RS11405 0.52 +2.3 755,692 - RPSI07_RS11405 0.52 -0.3 755,692 - RPSI07_RS11405 0.52 +1.6 755,734 - RPSI07_RS11405 0.57 +3.2 755,922 + RPSI07_RS11405 0.78 -0.7 755,979 - RPSI07_RS11405 0.84 +0.4 755,986 - RPSI07_RS11405 0.85 +0.2 755,986 - RPSI07_RS11405 0.85 -0.2 756,068 + +1.5 756,069 - -0.8 756,114 - +0.1 756,473 - RPSI07_RS11410 0.41 -0.4 756,818 - RPSI07_RS11410 0.80 +1.0 756,827 - RPSI07_RS11410 0.81 -0.2 756,827 - RPSI07_RS11410 0.81 +1.8 756,827 - RPSI07_RS11410 0.81 +2.0 756,827 - RPSI07_RS11410 0.81 +1.4 756,827 - RPSI07_RS11410 0.81 +0.5 756,827 - RPSI07_RS11410 0.81 -2.1 757,207 + RPSI07_RS11415 0.27 -0.2 757,533 + RPSI07_RS11415 0.68 -0.6 757,696 - RPSI07_RS11415 0.88 -0.7 757,780 - -2.3
Or see this region's nucleotide sequence