Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10645 and RPSI07_RS10650 are separated by 98 nucleotides RPSI07_RS10650 and RPSI07_RS10655 are separated by 208 nucleotides RPSI07_RS10655 and RPSI07_RS10660 are separated by 81 nucleotides
RPSI07_RS10645: RPSI07_RS10645 - peptide chain release factor 1, at 591,972 to 593,054
_RS10645
RPSI07_RS10650: RPSI07_RS10650 - glutamyl-tRNA reductase, at 593,153 to 594,460
_RS10650
RPSI07_RS10655: RPSI07_RS10655 - GlsB/YeaQ/YmgE family stress response membrane protein, at 594,669 to 594,911
_RS10655
RPSI07_RS10660: RPSI07_RS10660 - redox-regulated ATPase YchF, at 594,993 to 596,087
_RS10660
Position (kb) 593
594
595 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 593.100 kb on - strand at 594.514 kb on + strand at 594.515 kb on - strand at 594.515 kb on - strand at 594.515 kb on - strand at 594.573 kb on + strand at 594.574 kb on - strand at 594.580 kb on + strand at 594.581 kb on - strand at 594.589 kb on + strand at 594.590 kb on - strand at 594.629 kb on + strand at 594.629 kb on + strand at 594.629 kb on + strand at 594.630 kb on - strand at 594.630 kb on - strand at 594.662 kb on + strand at 594.689 kb on - strand at 594.825 kb on + strand, within RPSI07_RS10655 at 594.825 kb on + strand, within RPSI07_RS10655 at 594.826 kb on - strand, within RPSI07_RS10655 at 594.826 kb on - strand, within RPSI07_RS10655 at 595.039 kb on + strand at 595.039 kb on + strand at 595.040 kb on - strand at 595.040 kb on - strand at 595.040 kb on - strand at 595.096 kb on + strand at 595.114 kb on + strand, within RPSI07_RS10660 at 595.241 kb on - strand, within RPSI07_RS10660 at 595.412 kb on - strand, within RPSI07_RS10660
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 593,100 - -0.3 594,514 + -0.1 594,515 - -1.2 594,515 - +0.5 594,515 - +0.6 594,573 + -0.8 594,574 - +1.4 594,580 + -1.7 594,581 - -1.3 594,589 + -0.8 594,590 - +1.1 594,629 + -0.0 594,629 + -1.3 594,629 + +1.7 594,630 - +2.1 594,630 - +0.6 594,662 + -2.4 594,689 - +1.7 594,825 + RPSI07_RS10655 0.64 +2.6 594,825 + RPSI07_RS10655 0.64 -0.8 594,826 - RPSI07_RS10655 0.65 -0.8 594,826 - RPSI07_RS10655 0.65 +0.7 595,039 + +2.0 595,039 + +1.0 595,040 - -3.7 595,040 - -0.0 595,040 - -1.3 595,096 + +0.4 595,114 + RPSI07_RS10660 0.11 +1.0 595,241 - RPSI07_RS10660 0.23 +1.0 595,412 - RPSI07_RS10660 0.38 -0.0
Or see this region's nucleotide sequence