Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10345 and RPSI07_RS10350 are separated by 3 nucleotides RPSI07_RS10350 and RPSI07_RS10355 are separated by 154 nucleotides RPSI07_RS10355 and RPSI07_RS10360 are separated by 65 nucleotides RPSI07_RS10360 and RPSI07_RS10365 are separated by 236 nucleotides
RPSI07_RS10345: RPSI07_RS10345 - outer membrane lipid asymmetry maintenance protein MlaD, at 530,908 to 531,426
_RS10345
RPSI07_RS10350: RPSI07_RS10350 - ABC transporter, at 531,430 to 532,239
_RS10350
RPSI07_RS10355: RPSI07_RS10355 - hypothetical protein, at 532,394 to 533,029
_RS10355
RPSI07_RS10360: RPSI07_RS10360 - STAS domain-containing protein, at 533,095 to 533,409
_RS10360
RPSI07_RS10365: RPSI07_RS10365 - ABC transporter ATP-binding protein, at 533,646 to 534,611
_RS10365
Position (kb) 532
533
534 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 531.424 kb on + strand at 531.425 kb on - strand at 531.462 kb on + strand at 531.599 kb on + strand, within RPSI07_RS10350 at 531.599 kb on + strand, within RPSI07_RS10350 at 531.622 kb on + strand, within RPSI07_RS10350 at 531.622 kb on + strand, within RPSI07_RS10350 at 531.622 kb on + strand, within RPSI07_RS10350 at 531.691 kb on + strand, within RPSI07_RS10350 at 531.692 kb on - strand, within RPSI07_RS10350 at 531.877 kb on + strand, within RPSI07_RS10350 at 531.878 kb on - strand, within RPSI07_RS10350 at 531.961 kb on + strand, within RPSI07_RS10350 at 531.961 kb on + strand, within RPSI07_RS10350 at 531.962 kb on - strand, within RPSI07_RS10350 at 531.962 kb on - strand, within RPSI07_RS10350 at 531.969 kb on + strand, within RPSI07_RS10350 at 531.986 kb on - strand, within RPSI07_RS10350 at 532.038 kb on - strand, within RPSI07_RS10350 at 532.073 kb on - strand, within RPSI07_RS10350 at 532.120 kb on + strand, within RPSI07_RS10350 at 532.199 kb on - strand at 532.251 kb on + strand at 532.252 kb on - strand at 532.252 kb on - strand at 532.327 kb on + strand at 532.328 kb on - strand at 532.380 kb on + strand at 532.381 kb on - strand at 532.394 kb on - strand at 532.394 kb on - strand at 532.562 kb on - strand, within RPSI07_RS10355 at 532.761 kb on - strand, within RPSI07_RS10355 at 532.883 kb on + strand, within RPSI07_RS10355 at 533.027 kb on + strand at 533.028 kb on - strand at 533.028 kb on - strand at 533.042 kb on + strand at 533.342 kb on - strand, within RPSI07_RS10360 at 533.342 kb on - strand, within RPSI07_RS10360 at 533.539 kb on - strand at 533.552 kb on + strand at 533.635 kb on + strand at 533.635 kb on + strand at 533.637 kb on + strand at 533.637 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 531,424 + -2.5 531,425 - -2.1 531,462 + -0.0 531,599 + RPSI07_RS10350 0.21 -2.9 531,599 + RPSI07_RS10350 0.21 -3.8 531,622 + RPSI07_RS10350 0.24 -1.9 531,622 + RPSI07_RS10350 0.24 -1.6 531,622 + RPSI07_RS10350 0.24 -3.1 531,691 + RPSI07_RS10350 0.32 -2.4 531,692 - RPSI07_RS10350 0.32 -4.3 531,877 + RPSI07_RS10350 0.55 -3.3 531,878 - RPSI07_RS10350 0.55 -0.6 531,961 + RPSI07_RS10350 0.66 -0.0 531,961 + RPSI07_RS10350 0.66 -2.6 531,962 - RPSI07_RS10350 0.66 +1.0 531,962 - RPSI07_RS10350 0.66 -0.4 531,969 + RPSI07_RS10350 0.67 -4.2 531,986 - RPSI07_RS10350 0.69 -2.2 532,038 - RPSI07_RS10350 0.75 -2.9 532,073 - RPSI07_RS10350 0.79 -2.9 532,120 + RPSI07_RS10350 0.85 -2.7 532,199 - -0.7 532,251 + -0.6 532,252 - +0.8 532,252 - +2.0 532,327 + +0.0 532,328 - -2.8 532,380 + +0.6 532,381 - -4.6 532,394 - -1.4 532,394 - -3.7 532,562 - RPSI07_RS10355 0.26 +1.0 532,761 - RPSI07_RS10355 0.58 +1.0 532,883 + RPSI07_RS10355 0.77 -2.4 533,027 + +1.0 533,028 - +0.1 533,028 - -0.7 533,042 + -3.0 533,342 - RPSI07_RS10360 0.78 -3.3 533,342 - RPSI07_RS10360 0.78 -5.1 533,539 - -0.0 533,552 + +1.5 533,635 + -1.2 533,635 + +3.0 533,637 + -0.6 533,637 + -0.7
Or see this region's nucleotide sequence