Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS08155 and RPSI07_RS08160 are separated by 19 nucleotides RPSI07_RS08160 and RPSI07_RS08165 are separated by 160 nucleotides
RPSI07_RS08155: RPSI07_RS08155 - potassium-transporting ATPase subunit KdpA, at 71,477 to 73,249
_RS08155
RPSI07_RS08160: RPSI07_RS08160 - K(+)-transporting ATPase subunit F, at 73,269 to 73,358
_RS08160
RPSI07_RS08165: RPSI07_RS08165 - hypothetical protein, at 73,519 to 74,334
_RS08165
Position (kb) 73
74 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 72.426 kb on + strand, within RPSI07_RS08155 at 72.426 kb on + strand, within RPSI07_RS08155 at 72.427 kb on - strand, within RPSI07_RS08155 at 72.609 kb on + strand, within RPSI07_RS08155 at 72.609 kb on + strand, within RPSI07_RS08155 at 72.609 kb on + strand, within RPSI07_RS08155 at 72.642 kb on + strand, within RPSI07_RS08155 at 72.643 kb on - strand, within RPSI07_RS08155 at 72.717 kb on + strand, within RPSI07_RS08155 at 72.717 kb on + strand, within RPSI07_RS08155 at 72.717 kb on + strand, within RPSI07_RS08155 at 72.717 kb on + strand, within RPSI07_RS08155 at 72.717 kb on + strand, within RPSI07_RS08155 at 72.717 kb on + strand, within RPSI07_RS08155 at 72.717 kb on + strand, within RPSI07_RS08155 at 72.718 kb on - strand, within RPSI07_RS08155 at 72.718 kb on - strand, within RPSI07_RS08155 at 72.718 kb on - strand, within RPSI07_RS08155 at 72.718 kb on - strand, within RPSI07_RS08155 at 72.718 kb on - strand, within RPSI07_RS08155 at 72.719 kb on - strand, within RPSI07_RS08155 at 72.868 kb on - strand, within RPSI07_RS08155 at 72.868 kb on - strand, within RPSI07_RS08155 at 72.868 kb on - strand, within RPSI07_RS08155 at 72.868 kb on - strand, within RPSI07_RS08155 at 72.891 kb on + strand, within RPSI07_RS08155 at 72.891 kb on + strand, within RPSI07_RS08155 at 72.892 kb on - strand, within RPSI07_RS08155 at 73.011 kb on + strand, within RPSI07_RS08155 at 73.011 kb on + strand, within RPSI07_RS08155 at 73.011 kb on + strand, within RPSI07_RS08155 at 73.012 kb on - strand, within RPSI07_RS08155 at 73.012 kb on - strand, within RPSI07_RS08155 at 73.012 kb on - strand, within RPSI07_RS08155 at 73.012 kb on - strand, within RPSI07_RS08155 at 73.059 kb on + strand, within RPSI07_RS08155 at 73.059 kb on + strand, within RPSI07_RS08155 at 73.060 kb on - strand, within RPSI07_RS08155 at 73.107 kb on + strand at 73.108 kb on - strand at 73.108 kb on - strand at 73.176 kb on + strand at 73.176 kb on + strand at 73.176 kb on + strand at 73.176 kb on + strand at 73.176 kb on + strand at 73.176 kb on + strand at 73.176 kb on + strand at 73.176 kb on + strand at 73.176 kb on + strand at 73.177 kb on - strand at 73.177 kb on - strand at 73.177 kb on - strand at 73.177 kb on - strand at 73.177 kb on - strand at 73.218 kb on + strand at 73.218 kb on + strand at 73.219 kb on - strand at 73.219 kb on - strand at 73.306 kb on + strand, within RPSI07_RS08160 at 73.306 kb on + strand, within RPSI07_RS08160 at 73.306 kb on + strand, within RPSI07_RS08160 at 73.306 kb on + strand, within RPSI07_RS08160 at 73.345 kb on + strand, within RPSI07_RS08160 at 73.346 kb on - strand, within RPSI07_RS08160 at 73.346 kb on - strand, within RPSI07_RS08160 at 73.346 kb on - strand, within RPSI07_RS08160 at 73.346 kb on - strand, within RPSI07_RS08160 at 73.467 kb on + strand at 73.467 kb on + strand at 73.467 kb on + strand at 73.468 kb on - strand at 73.468 kb on - strand at 73.468 kb on - strand at 73.468 kb on - strand at 73.468 kb on - strand at 73.468 kb on - strand at 73.468 kb on - strand at 73.566 kb on - strand at 73.566 kb on - strand at 73.566 kb on - strand at 73.566 kb on - strand at 73.566 kb on - strand at 73.566 kb on - strand at 73.566 kb on - strand at 73.566 kb on - strand at 73.566 kb on - strand at 73.566 kb on - strand at 73.585 kb on - strand at 73.587 kb on - strand at 74.070 kb on - strand, within RPSI07_RS08165
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 72,426 + RPSI07_RS08155 0.54 +2.0 72,426 + RPSI07_RS08155 0.54 -0.3 72,427 - RPSI07_RS08155 0.54 -1.1 72,609 + RPSI07_RS08155 0.64 -0.5 72,609 + RPSI07_RS08155 0.64 -0.3 72,609 + RPSI07_RS08155 0.64 -1.0 72,642 + RPSI07_RS08155 0.66 +2.0 72,643 - RPSI07_RS08155 0.66 +0.4 72,717 + RPSI07_RS08155 0.70 +3.0 72,717 + RPSI07_RS08155 0.70 +0.2 72,717 + RPSI07_RS08155 0.70 -0.0 72,717 + RPSI07_RS08155 0.70 -2.4 72,717 + RPSI07_RS08155 0.70 -0.4 72,717 + RPSI07_RS08155 0.70 -0.3 72,717 + RPSI07_RS08155 0.70 -1.4 72,718 - RPSI07_RS08155 0.70 -0.0 72,718 - RPSI07_RS08155 0.70 +2.5 72,718 - RPSI07_RS08155 0.70 -3.8 72,718 - RPSI07_RS08155 0.70 -0.2 72,718 - RPSI07_RS08155 0.70 +0.4 72,719 - RPSI07_RS08155 0.70 +2.0 72,868 - RPSI07_RS08155 0.78 +3.3 72,868 - RPSI07_RS08155 0.78 +0.6 72,868 - RPSI07_RS08155 0.78 +1.4 72,868 - RPSI07_RS08155 0.78 +0.5 72,891 + RPSI07_RS08155 0.80 +0.2 72,891 + RPSI07_RS08155 0.80 +1.8 72,892 - RPSI07_RS08155 0.80 +0.5 73,011 + RPSI07_RS08155 0.87 -0.4 73,011 + RPSI07_RS08155 0.87 -0.4 73,011 + RPSI07_RS08155 0.87 +1.0 73,012 - RPSI07_RS08155 0.87 -0.7 73,012 - RPSI07_RS08155 0.87 -0.9 73,012 - RPSI07_RS08155 0.87 +1.2 73,012 - RPSI07_RS08155 0.87 -1.7 73,059 + RPSI07_RS08155 0.89 -2.3 73,059 + RPSI07_RS08155 0.89 +0.5 73,060 - RPSI07_RS08155 0.89 +0.6 73,107 + -0.2 73,108 - +0.2 73,108 - -0.6 73,176 + +3.1 73,176 + +0.5 73,176 + +1.0 73,176 + +2.5 73,176 + +1.0 73,176 + -0.0 73,176 + +0.6 73,176 + +2.4 73,176 + +0.0 73,177 - -0.9 73,177 - -1.2 73,177 - -1.4 73,177 - -0.5 73,177 - +0.4 73,218 + +1.0 73,218 + +0.2 73,219 - +1.6 73,219 - +0.3 73,306 + RPSI07_RS08160 0.41 -0.3 73,306 + RPSI07_RS08160 0.41 -1.0 73,306 + RPSI07_RS08160 0.41 -0.2 73,306 + RPSI07_RS08160 0.41 -0.9 73,345 + RPSI07_RS08160 0.84 -0.6 73,346 - RPSI07_RS08160 0.86 -0.4 73,346 - RPSI07_RS08160 0.86 +1.0 73,346 - RPSI07_RS08160 0.86 -0.7 73,346 - RPSI07_RS08160 0.86 -0.9 73,467 + -1.7 73,467 + -1.2 73,467 + +0.4 73,468 - +1.0 73,468 - +0.3 73,468 - -1.8 73,468 - -0.0 73,468 - +0.8 73,468 - +0.5 73,468 - +2.0 73,566 - +0.0 73,566 - +0.5 73,566 - -0.7 73,566 - -1.9 73,566 - -0.3 73,566 - +0.6 73,566 - +0.2 73,566 - -1.1 73,566 - +0.4 73,566 - +1.1 73,585 - +1.1 73,587 - +1.0 74,070 - RPSI07_RS08165 0.68 +2.5
Or see this region's nucleotide sequence