Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS07645 and RPSI07_RS07650 are separated by 124 nucleotides RPSI07_RS07650 and RPSI07_RS07655 are separated by 46 nucleotides
RPSI07_RS07645: RPSI07_RS07645 - sugar ABC transporter permease, at 2,036,230 to 2,037,426
_RS07645
RPSI07_RS07650: RPSI07_RS07650 - NAD(P)-dependent oxidoreductase, at 2,037,551 to 2,038,468
_RS07650
RPSI07_RS07655: RPSI07_RS07655 - LacI family DNA-binding transcriptional regulator, at 2,038,515 to 2,039,555
_RS07655
Position (kb)
2037
2038
2039 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2037.010 kb on + strand, within RPSI07_RS07645 at 2037.011 kb on - strand, within RPSI07_RS07645 at 2037.046 kb on + strand, within RPSI07_RS07645 at 2037.047 kb on - strand, within RPSI07_RS07645 at 2037.274 kb on + strand, within RPSI07_RS07645 at 2037.275 kb on - strand, within RPSI07_RS07645 at 2037.275 kb on - strand, within RPSI07_RS07645 at 2037.429 kb on + strand at 2037.429 kb on + strand at 2037.429 kb on + strand at 2037.429 kb on + strand at 2037.429 kb on + strand at 2037.430 kb on - strand at 2037.941 kb on + strand, within RPSI07_RS07650 at 2038.043 kb on + strand, within RPSI07_RS07650 at 2038.055 kb on + strand, within RPSI07_RS07650 at 2038.056 kb on - strand, within RPSI07_RS07650 at 2038.443 kb on - strand at 2038.558 kb on + strand at 2038.643 kb on - strand, within RPSI07_RS07655 at 2038.718 kb on - strand, within RPSI07_RS07655 at 2039.110 kb on + strand, within RPSI07_RS07655 at 2039.110 kb on - strand, within RPSI07_RS07655 at 2039.111 kb on - strand, within RPSI07_RS07655 at 2039.243 kb on - strand, within RPSI07_RS07655 at 2039.269 kb on + strand, within RPSI07_RS07655 at 2039.320 kb on + strand, within RPSI07_RS07655 at 2039.320 kb on + strand, within RPSI07_RS07655 at 2039.320 kb on + strand, within RPSI07_RS07655 at 2039.320 kb on + strand, within RPSI07_RS07655 at 2039.320 kb on + strand, within RPSI07_RS07655 at 2039.320 kb on + strand, within RPSI07_RS07655 at 2039.321 kb on - strand, within RPSI07_RS07655 at 2039.321 kb on - strand, within RPSI07_RS07655 at 2039.321 kb on - strand, within RPSI07_RS07655 at 2039.341 kb on + strand, within RPSI07_RS07655 at 2039.404 kb on + strand, within RPSI07_RS07655
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,037,010 + RPSI07_RS07645 0.65 +1.5 2,037,011 - RPSI07_RS07645 0.65 -1.3 2,037,046 + RPSI07_RS07645 0.68 -1.9 2,037,047 - RPSI07_RS07645 0.68 -0.3 2,037,274 + RPSI07_RS07645 0.87 -1.9 2,037,275 - RPSI07_RS07645 0.87 +0.3 2,037,275 - RPSI07_RS07645 0.87 +0.7 2,037,429 + -0.8 2,037,429 + +3.3 2,037,429 + +0.6 2,037,429 + -0.6 2,037,429 + +1.8 2,037,430 - -0.2 2,037,941 + RPSI07_RS07650 0.42 +0.3 2,038,043 + RPSI07_RS07650 0.54 -1.6 2,038,055 + RPSI07_RS07650 0.55 +0.1 2,038,056 - RPSI07_RS07650 0.55 -1.0 2,038,443 - +0.7 2,038,558 + -0.5 2,038,643 - RPSI07_RS07655 0.12 -1.0 2,038,718 - RPSI07_RS07655 0.20 -0.7 2,039,110 + RPSI07_RS07655 0.57 +0.7 2,039,110 - RPSI07_RS07655 0.57 +0.1 2,039,111 - RPSI07_RS07655 0.57 -1.0 2,039,243 - RPSI07_RS07655 0.70 +0.7 2,039,269 + RPSI07_RS07655 0.72 -0.0 2,039,320 + RPSI07_RS07655 0.77 +2.1 2,039,320 + RPSI07_RS07655 0.77 -0.8 2,039,320 + RPSI07_RS07655 0.77 -1.1 2,039,320 + RPSI07_RS07655 0.77 +1.0 2,039,320 + RPSI07_RS07655 0.77 -0.9 2,039,320 + RPSI07_RS07655 0.77 -2.1 2,039,321 - RPSI07_RS07655 0.77 -0.6 2,039,321 - RPSI07_RS07655 0.77 -1.8 2,039,321 - RPSI07_RS07655 0.77 -0.7 2,039,341 + RPSI07_RS07655 0.79 +2.7 2,039,404 + RPSI07_RS07655 0.85 -3.2
Or see this region's nucleotide sequence