Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS07285 and RPSI07_RS07290 are separated by 6 nucleotides RPSI07_RS07290 and RPSI07_RS07295 are separated by 135 nucleotides RPSI07_RS07295 and RPSI07_RS07300 are separated by 283 nucleotides
RPSI07_RS07285: RPSI07_RS07285 - two-component sensor histidine kinase, at 1,933,748 to 1,935,064
_RS07285
RPSI07_RS07290: RPSI07_RS07290 - DNA-binding response regulator, at 1,935,071 to 1,935,811
_RS07290
RPSI07_RS07295: RPSI07_RS07295 - hypothetical protein, at 1,935,947 to 1,938,151
_RS07295
RPSI07_RS07300: RPSI07_RS07300 - MurR/RpiR family transcriptional regulator, at 1,938,435 to 1,939,280
_RS07300
Position (kb)
1935
1936
1937
1938
1939 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1934.982 kb on + strand at 1934.982 kb on + strand at 1934.982 kb on + strand at 1934.982 kb on + strand at 1934.983 kb on - strand at 1934.983 kb on - strand at 1934.983 kb on - strand at 1934.983 kb on - strand at 1934.983 kb on - strand at 1934.983 kb on - strand at 1935.073 kb on - strand at 1935.135 kb on + strand at 1935.135 kb on + strand at 1935.135 kb on + strand at 1935.198 kb on + strand, within RPSI07_RS07290 at 1935.199 kb on - strand, within RPSI07_RS07290 at 1935.199 kb on - strand, within RPSI07_RS07290 at 1935.199 kb on - strand, within RPSI07_RS07290 at 1935.199 kb on - strand, within RPSI07_RS07290 at 1935.199 kb on - strand, within RPSI07_RS07290 at 1935.199 kb on - strand, within RPSI07_RS07290 at 1935.211 kb on + strand, within RPSI07_RS07290 at 1935.211 kb on + strand, within RPSI07_RS07290 at 1935.211 kb on + strand, within RPSI07_RS07290 at 1935.211 kb on + strand, within RPSI07_RS07290 at 1935.211 kb on + strand, within RPSI07_RS07290 at 1935.211 kb on + strand, within RPSI07_RS07290 at 1935.212 kb on - strand at 1935.212 kb on - strand, within RPSI07_RS07290 at 1935.212 kb on - strand, within RPSI07_RS07290 at 1935.212 kb on - strand, within RPSI07_RS07290 at 1935.240 kb on + strand, within RPSI07_RS07290 at 1935.241 kb on - strand, within RPSI07_RS07290 at 1935.483 kb on + strand, within RPSI07_RS07290 at 1935.483 kb on + strand, within RPSI07_RS07290 at 1935.484 kb on - strand, within RPSI07_RS07290 at 1935.803 kb on + strand at 1935.803 kb on + strand at 1935.803 kb on + strand at 1935.803 kb on + strand at 1935.803 kb on + strand at 1935.804 kb on - strand at 1935.848 kb on + strand at 1935.849 kb on - strand at 1935.849 kb on - strand at 1935.984 kb on + strand at 1935.985 kb on - strand at 1936.003 kb on + strand at 1936.003 kb on + strand at 1936.116 kb on + strand at 1936.116 kb on + strand at 1936.117 kb on - strand at 1936.117 kb on - strand at 1936.143 kb on + strand at 1936.144 kb on - strand at 1936.144 kb on - strand at 1936.144 kb on - strand at 1936.153 kb on + strand at 1936.154 kb on - strand at 1936.470 kb on + strand, within RPSI07_RS07295 at 1936.470 kb on + strand, within RPSI07_RS07295 at 1936.470 kb on + strand, within RPSI07_RS07295 at 1936.471 kb on - strand, within RPSI07_RS07295 at 1936.471 kb on - strand, within RPSI07_RS07295 at 1936.471 kb on - strand, within RPSI07_RS07295 at 1936.471 kb on - strand, within RPSI07_RS07295 at 1936.471 kb on - strand, within RPSI07_RS07295 at 1936.737 kb on + strand, within RPSI07_RS07295 at 1936.737 kb on + strand, within RPSI07_RS07295 at 1936.738 kb on - strand, within RPSI07_RS07295 at 1936.738 kb on - strand, within RPSI07_RS07295 at 1936.767 kb on + strand, within RPSI07_RS07295 at 1936.767 kb on + strand, within RPSI07_RS07295 at 1936.767 kb on + strand, within RPSI07_RS07295 at 1936.768 kb on - strand, within RPSI07_RS07295 at 1936.768 kb on - strand, within RPSI07_RS07295 at 1936.768 kb on - strand, within RPSI07_RS07295 at 1937.046 kb on + strand, within RPSI07_RS07295 at 1937.046 kb on + strand, within RPSI07_RS07295 at 1937.047 kb on - strand, within RPSI07_RS07295 at 1937.047 kb on - strand, within RPSI07_RS07295 at 1937.047 kb on - strand, within RPSI07_RS07295 at 1937.047 kb on - strand, within RPSI07_RS07295 at 1937.047 kb on - strand, within RPSI07_RS07295 at 1937.145 kb on + strand, within RPSI07_RS07295 at 1937.146 kb on - strand, within RPSI07_RS07295 at 1937.146 kb on - strand, within RPSI07_RS07295 at 1937.155 kb on - strand, within RPSI07_RS07295 at 1937.160 kb on + strand, within RPSI07_RS07295 at 1937.161 kb on - strand, within RPSI07_RS07295 at 1937.161 kb on - strand, within RPSI07_RS07295 at 1937.161 kb on - strand, within RPSI07_RS07295 at 1937.232 kb on + strand, within RPSI07_RS07295 at 1937.233 kb on - strand, within RPSI07_RS07295 at 1937.233 kb on - strand, within RPSI07_RS07295 at 1937.233 kb on - strand, within RPSI07_RS07295 at 1937.233 kb on - strand, within RPSI07_RS07295 at 1937.245 kb on - strand, within RPSI07_RS07295 at 1937.245 kb on - strand, within RPSI07_RS07295 at 1937.245 kb on - strand, within RPSI07_RS07295 at 1937.268 kb on + strand, within RPSI07_RS07295 at 1937.268 kb on + strand, within RPSI07_RS07295 at 1937.268 kb on + strand, within RPSI07_RS07295 at 1937.268 kb on + strand, within RPSI07_RS07295 at 1937.269 kb on - strand, within RPSI07_RS07295 at 1937.269 kb on - strand, within RPSI07_RS07295 at 1937.269 kb on - strand, within RPSI07_RS07295 at 1937.622 kb on + strand, within RPSI07_RS07295 at 1937.623 kb on - strand, within RPSI07_RS07295 at 1937.758 kb on - strand, within RPSI07_RS07295 at 1937.758 kb on - strand, within RPSI07_RS07295 at 1937.804 kb on - strand, within RPSI07_RS07295 at 1938.039 kb on + strand at 1938.039 kb on + strand at 1938.063 kb on - strand at 1938.145 kb on + strand at 1938.145 kb on + strand at 1938.145 kb on + strand at 1938.145 kb on + strand at 1938.146 kb on - strand at 1938.146 kb on - strand at 1938.146 kb on - strand at 1938.146 kb on - strand at 1938.162 kb on + strand at 1938.181 kb on + strand at 1938.181 kb on + strand at 1938.181 kb on + strand at 1938.181 kb on + strand at 1938.182 kb on - strand at 1938.182 kb on - strand at 1938.182 kb on - strand at 1938.207 kb on + strand at 1938.207 kb on + strand at 1938.207 kb on + strand at 1938.208 kb on - strand at 1938.219 kb on + strand at 1938.219 kb on + strand at 1938.219 kb on + strand at 1938.219 kb on + strand at 1938.219 kb on + strand at 1938.220 kb on - strand at 1938.220 kb on - strand at 1938.398 kb on + strand at 1938.398 kb on + strand at 1938.566 kb on - strand, within RPSI07_RS07300 at 1938.951 kb on + strand, within RPSI07_RS07300 at 1938.951 kb on + strand, within RPSI07_RS07300 at 1938.951 kb on + strand, within RPSI07_RS07300 at 1938.952 kb on - strand, within RPSI07_RS07300 at 1938.963 kb on + strand, within RPSI07_RS07300 at 1938.963 kb on + strand, within RPSI07_RS07300 at 1938.964 kb on - strand, within RPSI07_RS07300
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,934,982 + -0.2 1,934,982 + +0.3 1,934,982 + -2.3 1,934,982 + -0.3 1,934,983 - -2.3 1,934,983 - +1.7 1,934,983 - +1.9 1,934,983 - +0.0 1,934,983 - -0.3 1,934,983 - -0.7 1,935,073 - -1.1 1,935,135 + -0.1 1,935,135 + +0.3 1,935,135 + +0.8 1,935,198 + RPSI07_RS07290 0.17 +3.3 1,935,199 - RPSI07_RS07290 0.17 +0.9 1,935,199 - RPSI07_RS07290 0.17 -0.2 1,935,199 - RPSI07_RS07290 0.17 +0.0 1,935,199 - RPSI07_RS07290 0.17 -0.2 1,935,199 - RPSI07_RS07290 0.17 +1.1 1,935,199 - RPSI07_RS07290 0.17 -1.9 1,935,211 + RPSI07_RS07290 0.19 -2.7 1,935,211 + RPSI07_RS07290 0.19 +0.7 1,935,211 + RPSI07_RS07290 0.19 -2.2 1,935,211 + RPSI07_RS07290 0.19 -1.3 1,935,211 + RPSI07_RS07290 0.19 +1.2 1,935,211 + RPSI07_RS07290 0.19 -0.5 1,935,212 - -1.9 1,935,212 - RPSI07_RS07290 0.19 -1.8 1,935,212 - RPSI07_RS07290 0.19 +2.0 1,935,212 - RPSI07_RS07290 0.19 +0.3 1,935,240 + RPSI07_RS07290 0.23 +1.7 1,935,241 - RPSI07_RS07290 0.23 +4.8 1,935,483 + RPSI07_RS07290 0.56 -1.4 1,935,483 + RPSI07_RS07290 0.56 -0.7 1,935,484 - RPSI07_RS07290 0.56 -1.5 1,935,803 + -1.7 1,935,803 + -1.3 1,935,803 + +0.6 1,935,803 + -1.3 1,935,803 + +0.3 1,935,804 - -1.0 1,935,848 + -0.3 1,935,849 - -1.5 1,935,849 - -0.3 1,935,984 + -0.2 1,935,985 - +2.7 1,936,003 + -1.3 1,936,003 + +1.4 1,936,116 + +0.9 1,936,116 + -2.5 1,936,117 - -0.6 1,936,117 - +2.3 1,936,143 + -0.3 1,936,144 - +4.5 1,936,144 - -0.5 1,936,144 - -0.2 1,936,153 + -0.7 1,936,154 - -0.9 1,936,470 + RPSI07_RS07295 0.24 +0.7 1,936,470 + RPSI07_RS07295 0.24 +0.4 1,936,470 + RPSI07_RS07295 0.24 -2.3 1,936,471 - RPSI07_RS07295 0.24 -0.3 1,936,471 - RPSI07_RS07295 0.24 +3.2 1,936,471 - RPSI07_RS07295 0.24 +0.7 1,936,471 - RPSI07_RS07295 0.24 -0.8 1,936,471 - RPSI07_RS07295 0.24 -0.5 1,936,737 + RPSI07_RS07295 0.36 -0.3 1,936,737 + RPSI07_RS07295 0.36 +0.2 1,936,738 - RPSI07_RS07295 0.36 +2.7 1,936,738 - RPSI07_RS07295 0.36 -0.7 1,936,767 + RPSI07_RS07295 0.37 +0.2 1,936,767 + RPSI07_RS07295 0.37 +0.3 1,936,767 + RPSI07_RS07295 0.37 -0.8 1,936,768 - RPSI07_RS07295 0.37 +0.4 1,936,768 - RPSI07_RS07295 0.37 +0.1 1,936,768 - RPSI07_RS07295 0.37 -0.4 1,937,046 + RPSI07_RS07295 0.50 -0.9 1,937,046 + RPSI07_RS07295 0.50 -0.4 1,937,047 - RPSI07_RS07295 0.50 -0.4 1,937,047 - RPSI07_RS07295 0.50 -1.0 1,937,047 - RPSI07_RS07295 0.50 +1.8 1,937,047 - RPSI07_RS07295 0.50 +0.2 1,937,047 - RPSI07_RS07295 0.50 -0.3 1,937,145 + RPSI07_RS07295 0.54 -3.1 1,937,146 - RPSI07_RS07295 0.54 -1.8 1,937,146 - RPSI07_RS07295 0.54 -0.3 1,937,155 - RPSI07_RS07295 0.55 +0.4 1,937,160 + RPSI07_RS07295 0.55 +2.7 1,937,161 - RPSI07_RS07295 0.55 -0.4 1,937,161 - RPSI07_RS07295 0.55 -0.2 1,937,161 - RPSI07_RS07295 0.55 +0.9 1,937,232 + RPSI07_RS07295 0.58 +0.2 1,937,233 - RPSI07_RS07295 0.58 -0.1 1,937,233 - RPSI07_RS07295 0.58 +0.6 1,937,233 - RPSI07_RS07295 0.58 -0.4 1,937,233 - RPSI07_RS07295 0.58 -2.0 1,937,245 - RPSI07_RS07295 0.59 -0.4 1,937,245 - RPSI07_RS07295 0.59 -0.7 1,937,245 - RPSI07_RS07295 0.59 +0.5 1,937,268 + RPSI07_RS07295 0.60 +1.2 1,937,268 + RPSI07_RS07295 0.60 -0.9 1,937,268 + RPSI07_RS07295 0.60 +0.2 1,937,268 + RPSI07_RS07295 0.60 -2.1 1,937,269 - RPSI07_RS07295 0.60 +0.1 1,937,269 - RPSI07_RS07295 0.60 +0.1 1,937,269 - RPSI07_RS07295 0.60 +2.4 1,937,622 + RPSI07_RS07295 0.76 +0.1 1,937,623 - RPSI07_RS07295 0.76 +0.3 1,937,758 - RPSI07_RS07295 0.82 -0.3 1,937,758 - RPSI07_RS07295 0.82 -0.1 1,937,804 - RPSI07_RS07295 0.84 -2.0 1,938,039 + +1.7 1,938,039 + -0.7 1,938,063 - -0.7 1,938,145 + -0.3 1,938,145 + -1.2 1,938,145 + +0.4 1,938,145 + -0.4 1,938,146 - +2.0 1,938,146 - +0.1 1,938,146 - +2.3 1,938,146 - +0.3 1,938,162 + -2.9 1,938,181 + -0.7 1,938,181 + -0.6 1,938,181 + +0.0 1,938,181 + -1.2 1,938,182 - +0.3 1,938,182 - -1.3 1,938,182 - +1.0 1,938,207 + +0.7 1,938,207 + -0.4 1,938,207 + +2.8 1,938,208 - -1.7 1,938,219 + -1.3 1,938,219 + -1.1 1,938,219 + -1.2 1,938,219 + +1.6 1,938,219 + +1.7 1,938,220 - -1.1 1,938,220 - -1.7 1,938,398 + +0.3 1,938,398 + +0.1 1,938,566 - RPSI07_RS07300 0.15 -1.1 1,938,951 + RPSI07_RS07300 0.61 -0.3 1,938,951 + RPSI07_RS07300 0.61 -0.4 1,938,951 + RPSI07_RS07300 0.61 +0.7 1,938,952 - RPSI07_RS07300 0.61 -1.3 1,938,963 + RPSI07_RS07300 0.62 +2.6 1,938,963 + RPSI07_RS07300 0.62 -0.3 1,938,964 - RPSI07_RS07300 0.63 +2.3
Or see this region's nucleotide sequence