Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS05695 and RPSI07_RS05700 are separated by 197 nucleotides RPSI07_RS05700 and RPSI07_RS05705 are separated by 471 nucleotides
RPSI07_RS05695: RPSI07_RS05695 - LysR family transcriptional regulator, at 1,558,402 to 1,559,322
_RS05695
RPSI07_RS05700: RPSI07_RS05700 - polyisoprenoid-binding protein, at 1,559,520 to 1,560,095
_RS05700
RPSI07_RS05705: RPSI07_RS05705 - hypothetical protein, at 1,560,567 to 1,563,398
_RS05705
Position (kb) 1559
1560
1561 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1558.805 kb on + strand, within RPSI07_RS05695 at 1558.806 kb on - strand, within RPSI07_RS05695 at 1558.820 kb on + strand, within RPSI07_RS05695 at 1558.820 kb on + strand, within RPSI07_RS05695 at 1558.820 kb on + strand, within RPSI07_RS05695 at 1558.820 kb on + strand, within RPSI07_RS05695 at 1558.821 kb on - strand, within RPSI07_RS05695 at 1558.821 kb on - strand, within RPSI07_RS05695 at 1558.821 kb on - strand, within RPSI07_RS05695 at 1558.821 kb on - strand, within RPSI07_RS05695 at 1558.821 kb on - strand, within RPSI07_RS05695 at 1558.964 kb on + strand, within RPSI07_RS05695 at 1559.028 kb on + strand, within RPSI07_RS05695 at 1559.029 kb on - strand, within RPSI07_RS05695 at 1559.029 kb on - strand, within RPSI07_RS05695 at 1559.029 kb on - strand, within RPSI07_RS05695 at 1559.151 kb on - strand, within RPSI07_RS05695 at 1559.351 kb on + strand at 1559.351 kb on + strand at 1559.351 kb on + strand at 1559.352 kb on - strand at 1559.664 kb on - strand, within RPSI07_RS05700 at 1559.872 kb on - strand, within RPSI07_RS05700 at 1559.872 kb on - strand, within RPSI07_RS05700 at 1560.258 kb on - strand at 1560.354 kb on - strand at 1560.375 kb on - strand at 1560.446 kb on - strand at 1560.539 kb on - strand at 1560.566 kb on + strand at 1560.566 kb on + strand at 1560.567 kb on - strand at 1560.578 kb on + strand at 1560.578 kb on + strand at 1560.578 kb on + strand at 1560.578 kb on + strand at 1560.578 kb on + strand at 1560.579 kb on - strand at 1560.579 kb on - strand at 1560.918 kb on + strand, within RPSI07_RS05705 at 1560.918 kb on + strand, within RPSI07_RS05705 at 1560.936 kb on + strand, within RPSI07_RS05705 at 1560.936 kb on + strand, within RPSI07_RS05705 at 1560.936 kb on + strand, within RPSI07_RS05705 at 1560.936 kb on + strand, within RPSI07_RS05705 at 1560.936 kb on + strand, within RPSI07_RS05705 at 1560.936 kb on + strand, within RPSI07_RS05705 at 1560.936 kb on + strand, within RPSI07_RS05705 at 1560.936 kb on + strand, within RPSI07_RS05705 at 1560.937 kb on - strand, within RPSI07_RS05705 at 1560.937 kb on - strand, within RPSI07_RS05705 at 1560.950 kb on + strand, within RPSI07_RS05705 at 1560.950 kb on + strand, within RPSI07_RS05705
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,558,805 + RPSI07_RS05695 0.44 -1.3 1,558,806 - RPSI07_RS05695 0.44 +1.4 1,558,820 + RPSI07_RS05695 0.45 -0.1 1,558,820 + RPSI07_RS05695 0.45 -2.0 1,558,820 + RPSI07_RS05695 0.45 -1.1 1,558,820 + RPSI07_RS05695 0.45 +0.1 1,558,821 - RPSI07_RS05695 0.45 -1.1 1,558,821 - RPSI07_RS05695 0.45 -1.8 1,558,821 - RPSI07_RS05695 0.45 -1.0 1,558,821 - RPSI07_RS05695 0.45 +1.0 1,558,821 - RPSI07_RS05695 0.45 +2.3 1,558,964 + RPSI07_RS05695 0.61 -1.6 1,559,028 + RPSI07_RS05695 0.68 -1.0 1,559,029 - RPSI07_RS05695 0.68 +3.1 1,559,029 - RPSI07_RS05695 0.68 -2.1 1,559,029 - RPSI07_RS05695 0.68 +0.7 1,559,151 - RPSI07_RS05695 0.81 -0.4 1,559,351 + +2.6 1,559,351 + -0.1 1,559,351 + -1.1 1,559,352 - +3.0 1,559,664 - RPSI07_RS05700 0.25 -0.3 1,559,872 - RPSI07_RS05700 0.61 +2.0 1,559,872 - RPSI07_RS05700 0.61 -0.0 1,560,258 - +0.1 1,560,354 - -1.3 1,560,375 - -0.9 1,560,446 - -0.6 1,560,539 - -2.1 1,560,566 + -0.1 1,560,566 + -0.7 1,560,567 - -0.9 1,560,578 + -0.0 1,560,578 + +1.6 1,560,578 + +2.3 1,560,578 + +0.3 1,560,578 + -0.4 1,560,579 - +0.9 1,560,579 - +0.1 1,560,918 + RPSI07_RS05705 0.12 -0.7 1,560,918 + RPSI07_RS05705 0.12 +1.3 1,560,936 + RPSI07_RS05705 0.13 -0.2 1,560,936 + RPSI07_RS05705 0.13 -1.0 1,560,936 + RPSI07_RS05705 0.13 -0.9 1,560,936 + RPSI07_RS05705 0.13 -2.5 1,560,936 + RPSI07_RS05705 0.13 -0.3 1,560,936 + RPSI07_RS05705 0.13 -1.0 1,560,936 + RPSI07_RS05705 0.13 +0.7 1,560,936 + RPSI07_RS05705 0.13 +2.7 1,560,937 - RPSI07_RS05705 0.13 -0.9 1,560,937 - RPSI07_RS05705 0.13 -3.2 1,560,950 + RPSI07_RS05705 0.14 +1.2 1,560,950 + RPSI07_RS05705 0.14 -0.4
Or see this region's nucleotide sequence