Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS04645 and RPSI07_RS04650 are separated by 251 nucleotides RPSI07_RS04650 and RPSI07_RS04655 are separated by 74 nucleotides
RPSI07_RS04645: RPSI07_RS04645 - aldehyde dehydrogenase, at 1,257,200 to 1,258,675
_RS04645
RPSI07_RS04650: RPSI07_RS04650 - N-acetyltransferase, at 1,258,927 to 1,259,814
_RS04650
RPSI07_RS04655: RPSI07_RS04655 - glyoxylate/hydroxypyruvate reductase A, at 1,259,889 to 1,260,821
_RS04655
Position (kb)
1258
1259
1260 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1258.451 kb on + strand, within RPSI07_RS04645 at 1258.451 kb on + strand, within RPSI07_RS04645 at 1258.730 kb on - strand at 1258.739 kb on - strand at 1258.767 kb on + strand at 1258.768 kb on - strand at 1258.882 kb on + strand at 1258.882 kb on + strand at 1258.882 kb on + strand at 1258.882 kb on + strand at 1258.883 kb on - strand at 1258.883 kb on - strand at 1258.883 kb on - strand at 1259.294 kb on - strand, within RPSI07_RS04650 at 1259.294 kb on - strand, within RPSI07_RS04650 at 1259.886 kb on - strand at 1259.904 kb on + strand at 1259.907 kb on + strand at 1259.907 kb on + strand at 1259.907 kb on + strand at 1259.907 kb on + strand at 1259.907 kb on + strand at 1259.908 kb on - strand at 1259.908 kb on - strand at 1259.908 kb on - strand at 1259.908 kb on - strand at 1259.908 kb on - strand at 1259.909 kb on + strand at 1259.909 kb on + strand at 1259.909 kb on + strand at 1259.909 kb on + strand at 1259.909 kb on + strand at 1259.910 kb on - strand at 1259.910 kb on - strand at 1260.013 kb on + strand, within RPSI07_RS04655 at 1260.013 kb on + strand, within RPSI07_RS04655 at 1260.013 kb on + strand, within RPSI07_RS04655 at 1260.014 kb on - strand, within RPSI07_RS04655 at 1260.186 kb on + strand, within RPSI07_RS04655 at 1260.186 kb on + strand, within RPSI07_RS04655 at 1260.186 kb on + strand, within RPSI07_RS04655 at 1260.187 kb on - strand, within RPSI07_RS04655 at 1260.187 kb on - strand, within RPSI07_RS04655
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,258,451 + RPSI07_RS04645 0.85 +0.1 1,258,451 + RPSI07_RS04645 0.85 -2.8 1,258,730 - -1.8 1,258,739 - +0.6 1,258,767 + -0.7 1,258,768 - -1.8 1,258,882 + +1.5 1,258,882 + -0.6 1,258,882 + +0.5 1,258,882 + -0.3 1,258,883 - +0.7 1,258,883 - -0.1 1,258,883 - -0.9 1,259,294 - RPSI07_RS04650 0.41 +0.9 1,259,294 - RPSI07_RS04650 0.41 +0.8 1,259,886 - -1.0 1,259,904 + -0.9 1,259,907 + -1.1 1,259,907 + -1.0 1,259,907 + -0.1 1,259,907 + +0.4 1,259,907 + -1.3 1,259,908 - -0.1 1,259,908 - +0.2 1,259,908 - +0.3 1,259,908 - +0.3 1,259,908 - +2.1 1,259,909 + -0.6 1,259,909 + -0.2 1,259,909 + -1.2 1,259,909 + +0.1 1,259,909 + -0.5 1,259,910 - -0.9 1,259,910 - -2.0 1,260,013 + RPSI07_RS04655 0.13 -0.3 1,260,013 + RPSI07_RS04655 0.13 -1.5 1,260,013 + RPSI07_RS04655 0.13 -0.8 1,260,014 - RPSI07_RS04655 0.13 -0.2 1,260,186 + RPSI07_RS04655 0.32 -1.0 1,260,186 + RPSI07_RS04655 0.32 +0.7 1,260,186 + RPSI07_RS04655 0.32 +1.4 1,260,187 - RPSI07_RS04655 0.32 +1.7 1,260,187 - RPSI07_RS04655 0.32 -2.9
Or see this region's nucleotide sequence