Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS04505 and RPSI07_RS04510 are separated by 26 nucleotides RPSI07_RS04510 and RPSI07_RS04515 are separated by 241 nucleotides
RPSI07_RS04505: RPSI07_RS04505 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 1,216,299 to 1,217,570
_RS04505
RPSI07_RS04510: RPSI07_RS04510 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 1,217,597 to 1,218,724
_RS04510
RPSI07_RS04515: RPSI07_RS04515 - tyrosine protein kinase, at 1,218,966 to 1,221,215
_RS04515
Position (kb)
1217
1218
1219 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1216.979 kb on - strand, within RPSI07_RS04505 at 1216.979 kb on - strand, within RPSI07_RS04505 at 1217.263 kb on + strand, within RPSI07_RS04505 at 1217.263 kb on + strand, within RPSI07_RS04505 at 1217.263 kb on + strand, within RPSI07_RS04505 at 1217.263 kb on + strand, within RPSI07_RS04505 at 1217.263 kb on + strand, within RPSI07_RS04505 at 1217.264 kb on - strand, within RPSI07_RS04505 at 1217.357 kb on + strand, within RPSI07_RS04505 at 1217.357 kb on - strand, within RPSI07_RS04505 at 1217.515 kb on + strand at 1217.515 kb on + strand at 1217.515 kb on + strand at 1217.515 kb on + strand at 1217.515 kb on + strand at 1217.515 kb on + strand at 1217.515 kb on + strand at 1217.516 kb on - strand at 1217.516 kb on - strand at 1217.564 kb on + strand at 1217.564 kb on + strand at 1217.564 kb on + strand at 1217.564 kb on + strand at 1217.564 kb on + strand at 1217.564 kb on + strand at 1217.565 kb on - strand at 1217.565 kb on - strand at 1217.607 kb on + strand at 1217.607 kb on + strand at 1217.607 kb on + strand at 1217.607 kb on + strand at 1217.608 kb on - strand at 1217.656 kb on - strand at 1217.685 kb on + strand at 1217.685 kb on + strand at 1217.686 kb on - strand at 1217.889 kb on + strand, within RPSI07_RS04510 at 1217.904 kb on + strand, within RPSI07_RS04510 at 1217.904 kb on + strand, within RPSI07_RS04510 at 1217.905 kb on - strand, within RPSI07_RS04510 at 1218.123 kb on + strand, within RPSI07_RS04510 at 1218.376 kb on + strand, within RPSI07_RS04510 at 1218.399 kb on + strand, within RPSI07_RS04510 at 1218.399 kb on + strand, within RPSI07_RS04510 at 1218.399 kb on + strand, within RPSI07_RS04510 at 1218.399 kb on + strand, within RPSI07_RS04510 at 1218.400 kb on - strand, within RPSI07_RS04510 at 1218.402 kb on + strand, within RPSI07_RS04510 at 1218.403 kb on - strand, within RPSI07_RS04510 at 1218.436 kb on + strand, within RPSI07_RS04510 at 1218.437 kb on - strand, within RPSI07_RS04510 at 1218.519 kb on + strand, within RPSI07_RS04510 at 1218.520 kb on - strand, within RPSI07_RS04510 at 1218.520 kb on - strand, within RPSI07_RS04510 at 1218.520 kb on - strand, within RPSI07_RS04510 at 1218.555 kb on + strand, within RPSI07_RS04510 at 1218.556 kb on - strand, within RPSI07_RS04510 at 1218.556 kb on - strand, within RPSI07_RS04510 at 1218.556 kb on - strand, within RPSI07_RS04510 at 1218.698 kb on - strand at 1218.704 kb on + strand at 1218.705 kb on - strand at 1218.724 kb on + strand at 1218.725 kb on - strand at 1218.725 kb on - strand at 1219.004 kb on - strand at 1219.009 kb on + strand at 1219.025 kb on - strand at 1219.046 kb on - strand at 1219.057 kb on + strand at 1219.057 kb on + strand at 1219.057 kb on + strand at 1219.058 kb on - strand at 1219.058 kb on - strand at 1219.058 kb on - strand at 1219.146 kb on + strand at 1219.147 kb on - strand at 1219.238 kb on + strand, within RPSI07_RS04515 at 1219.259 kb on - strand, within RPSI07_RS04515 at 1219.274 kb on + strand, within RPSI07_RS04515 at 1219.274 kb on + strand, within RPSI07_RS04515 at 1219.274 kb on + strand, within RPSI07_RS04515 at 1219.275 kb on - strand, within RPSI07_RS04515 at 1219.460 kb on - strand, within RPSI07_RS04515 at 1219.682 kb on - strand, within RPSI07_RS04515 at 1219.682 kb on - strand, within RPSI07_RS04515
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,216,979 - RPSI07_RS04505 0.53 +0.1 1,216,979 - RPSI07_RS04505 0.53 +2.7 1,217,263 + RPSI07_RS04505 0.76 -0.1 1,217,263 + RPSI07_RS04505 0.76 -3.4 1,217,263 + RPSI07_RS04505 0.76 +0.0 1,217,263 + RPSI07_RS04505 0.76 +0.4 1,217,263 + RPSI07_RS04505 0.76 +0.5 1,217,264 - RPSI07_RS04505 0.76 -0.5 1,217,357 + RPSI07_RS04505 0.83 -1.1 1,217,357 - RPSI07_RS04505 0.83 -1.2 1,217,515 + -1.2 1,217,515 + -0.7 1,217,515 + -1.4 1,217,515 + -1.3 1,217,515 + +0.3 1,217,515 + -0.1 1,217,515 + +0.1 1,217,516 - -1.1 1,217,516 - -0.3 1,217,564 + +1.1 1,217,564 + -1.4 1,217,564 + -0.5 1,217,564 + -1.3 1,217,564 + -0.7 1,217,564 + -1.6 1,217,565 - -0.4 1,217,565 - -2.3 1,217,607 + +0.6 1,217,607 + -0.7 1,217,607 + +0.0 1,217,607 + -1.6 1,217,608 - -1.3 1,217,656 - +0.2 1,217,685 + -0.4 1,217,685 + +0.5 1,217,686 - -0.3 1,217,889 + RPSI07_RS04510 0.26 -0.7 1,217,904 + RPSI07_RS04510 0.27 -1.6 1,217,904 + RPSI07_RS04510 0.27 +1.9 1,217,905 - RPSI07_RS04510 0.27 -0.1 1,218,123 + RPSI07_RS04510 0.47 -0.5 1,218,376 + RPSI07_RS04510 0.69 -0.7 1,218,399 + RPSI07_RS04510 0.71 +0.4 1,218,399 + RPSI07_RS04510 0.71 +0.3 1,218,399 + RPSI07_RS04510 0.71 +0.2 1,218,399 + RPSI07_RS04510 0.71 +0.9 1,218,400 - RPSI07_RS04510 0.71 -2.3 1,218,402 + RPSI07_RS04510 0.71 -0.7 1,218,403 - RPSI07_RS04510 0.71 +1.2 1,218,436 + RPSI07_RS04510 0.74 +1.7 1,218,437 - RPSI07_RS04510 0.74 -0.3 1,218,519 + RPSI07_RS04510 0.82 -0.1 1,218,520 - RPSI07_RS04510 0.82 +0.2 1,218,520 - RPSI07_RS04510 0.82 +0.5 1,218,520 - RPSI07_RS04510 0.82 +0.5 1,218,555 + RPSI07_RS04510 0.85 -1.7 1,218,556 - RPSI07_RS04510 0.85 -0.8 1,218,556 - RPSI07_RS04510 0.85 +0.6 1,218,556 - RPSI07_RS04510 0.85 -0.3 1,218,698 - +1.0 1,218,704 + -0.4 1,218,705 - +0.3 1,218,724 + -0.7 1,218,725 - +1.7 1,218,725 - -0.2 1,219,004 - -0.6 1,219,009 + +1.9 1,219,025 - -1.6 1,219,046 - -0.7 1,219,057 + +0.5 1,219,057 + -0.3 1,219,057 + +1.9 1,219,058 - -1.2 1,219,058 - -1.7 1,219,058 - -0.9 1,219,146 + +1.8 1,219,147 - -0.8 1,219,238 + RPSI07_RS04515 0.12 -0.4 1,219,259 - RPSI07_RS04515 0.13 -0.3 1,219,274 + RPSI07_RS04515 0.14 +0.8 1,219,274 + RPSI07_RS04515 0.14 -1.9 1,219,274 + RPSI07_RS04515 0.14 -1.0 1,219,275 - RPSI07_RS04515 0.14 -0.2 1,219,460 - RPSI07_RS04515 0.22 -1.7 1,219,682 - RPSI07_RS04515 0.32 +2.3 1,219,682 - RPSI07_RS04515 0.32 -0.3
Or see this region's nucleotide sequence