Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS03275 and RPSI07_RS03280 are separated by 77 nucleotides RPSI07_RS03280 and RPSI07_RS03285 are separated by 229 nucleotides RPSI07_RS03285 and RPSI07_RS03290 are separated by 398 nucleotides
RPSI07_RS03275: RPSI07_RS03275 - alpha/beta hydrolase, at 893,295 to 894,167
_RS03275
RPSI07_RS03280: RPSI07_RS03280 - TetR/AcrR family transcriptional regulator, at 894,245 to 894,820
_RS03280
RPSI07_RS03285: RPSI07_RS03285 - MFS transporter, at 895,050 to 896,279
_RS03285
RPSI07_RS03290: RPSI07_RS03290 - translesion DNA synthesis-associated protein ImuA, at 896,678 to 897,613
_RS03290
Position (kb) 895
896
897 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 894.101 kb on - strand at 894.101 kb on - strand at 894.121 kb on + strand at 894.121 kb on + strand at 894.121 kb on + strand at 894.121 kb on + strand at 894.122 kb on - strand at 894.122 kb on - strand at 894.122 kb on - strand at 894.122 kb on - strand at 894.122 kb on - strand at 894.122 kb on - strand at 894.122 kb on - strand at 894.169 kb on + strand at 894.169 kb on + strand at 894.170 kb on + strand at 894.170 kb on + strand at 894.170 kb on + strand at 894.170 kb on + strand at 894.170 kb on + strand at 894.170 kb on + strand at 894.170 kb on + strand at 894.170 kb on + strand at 894.170 kb on + strand at 894.170 kb on + strand at 894.171 kb on - strand at 894.171 kb on - strand at 894.171 kb on - strand at 894.171 kb on - strand at 894.171 kb on - strand at 894.171 kb on - strand at 894.171 kb on - strand at 894.171 kb on - strand at 894.171 kb on - strand at 894.181 kb on - strand at 894.203 kb on + strand at 894.204 kb on - strand at 894.238 kb on - strand at 894.238 kb on - strand at 894.238 kb on - strand at 894.377 kb on + strand, within RPSI07_RS03280 at 894.377 kb on + strand, within RPSI07_RS03280 at 894.378 kb on - strand, within RPSI07_RS03280 at 894.378 kb on - strand, within RPSI07_RS03280 at 894.378 kb on - strand, within RPSI07_RS03280 at 894.378 kb on - strand, within RPSI07_RS03280 at 894.835 kb on + strand at 894.836 kb on - strand at 894.892 kb on + strand at 894.893 kb on - strand at 894.925 kb on + strand at 894.925 kb on + strand at 894.925 kb on + strand at 894.925 kb on + strand at 894.925 kb on + strand at 894.926 kb on - strand at 894.927 kb on + strand at 894.927 kb on + strand at 894.927 kb on + strand at 894.927 kb on + strand at 894.927 kb on + strand at 894.927 kb on + strand at 894.927 kb on + strand at 894.927 kb on + strand at 894.928 kb on - strand at 894.928 kb on - strand at 894.928 kb on - strand at 894.928 kb on - strand at 894.928 kb on - strand at 894.928 kb on - strand at 895.025 kb on + strand at 895.026 kb on - strand at 895.026 kb on - strand at 895.026 kb on - strand at 895.026 kb on - strand at 895.043 kb on + strand at 895.044 kb on - strand at 895.044 kb on - strand at 895.044 kb on - strand at 895.044 kb on - strand at 895.088 kb on + strand at 895.088 kb on + strand at 895.088 kb on + strand at 895.088 kb on + strand at 895.089 kb on - strand at 895.089 kb on - strand at 895.294 kb on - strand, within RPSI07_RS03285 at 895.390 kb on - strand, within RPSI07_RS03285 at 895.491 kb on + strand, within RPSI07_RS03285 at 895.491 kb on + strand, within RPSI07_RS03285 at 895.491 kb on + strand, within RPSI07_RS03285 at 895.491 kb on + strand, within RPSI07_RS03285 at 895.492 kb on - strand, within RPSI07_RS03285 at 895.556 kb on + strand, within RPSI07_RS03285 at 895.556 kb on + strand, within RPSI07_RS03285 at 895.557 kb on + strand, within RPSI07_RS03285 at 895.557 kb on + strand, within RPSI07_RS03285 at 895.558 kb on - strand, within RPSI07_RS03285 at 895.721 kb on + strand, within RPSI07_RS03285 at 895.722 kb on - strand, within RPSI07_RS03285 at 895.722 kb on - strand, within RPSI07_RS03285 at 895.722 kb on - strand, within RPSI07_RS03285 at 895.767 kb on + strand, within RPSI07_RS03285 at 895.770 kb on + strand, within RPSI07_RS03285 at 895.770 kb on + strand, within RPSI07_RS03285 at 895.770 kb on + strand, within RPSI07_RS03285 at 895.771 kb on - strand, within RPSI07_RS03285 at 895.797 kb on + strand, within RPSI07_RS03285 at 895.797 kb on + strand, within RPSI07_RS03285 at 895.797 kb on + strand, within RPSI07_RS03285 at 895.797 kb on + strand, within RPSI07_RS03285 at 895.798 kb on - strand, within RPSI07_RS03285 at 895.798 kb on - strand, within RPSI07_RS03285 at 895.798 kb on - strand, within RPSI07_RS03285 at 896.094 kb on - strand, within RPSI07_RS03285 at 896.165 kb on - strand at 896.165 kb on - strand at 896.165 kb on - strand at 896.165 kb on - strand at 896.165 kb on - strand at 896.165 kb on - strand at 896.165 kb on - strand at 896.176 kb on - strand at 896.214 kb on + strand at 896.214 kb on + strand at 896.214 kb on + strand at 896.214 kb on + strand at 896.214 kb on + strand at 896.215 kb on - strand at 896.215 kb on - strand at 896.215 kb on - strand at 896.225 kb on + strand at 896.225 kb on + strand at 896.225 kb on + strand at 896.226 kb on - strand at 896.226 kb on - strand at 896.296 kb on + strand at 896.296 kb on + strand at 896.296 kb on + strand at 896.297 kb on - strand at 896.492 kb on + strand at 896.493 kb on - strand at 896.637 kb on + strand at 897.039 kb on - strand, within RPSI07_RS03290
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 894,101 - -0.7 894,101 - +0.4 894,121 + -0.0 894,121 + -0.1 894,121 + +0.5 894,121 + +0.7 894,122 - -0.3 894,122 - -0.2 894,122 - -0.5 894,122 - -0.7 894,122 - +1.1 894,122 - -0.2 894,122 - +0.2 894,169 + +0.7 894,169 + -0.3 894,170 + -0.8 894,170 + -0.1 894,170 + +1.2 894,170 + +2.0 894,170 + -0.3 894,170 + +0.5 894,170 + +0.5 894,170 + +1.0 894,170 + +0.5 894,170 + -0.6 894,171 - +0.7 894,171 - +0.9 894,171 - -1.7 894,171 - -0.1 894,171 - -2.1 894,171 - +0.3 894,171 - +0.5 894,171 - -1.5 894,171 - -0.4 894,181 - -0.6 894,203 + -0.5 894,204 - +2.7 894,238 - +0.3 894,238 - -0.2 894,238 - -0.7 894,377 + RPSI07_RS03280 0.23 -0.2 894,377 + RPSI07_RS03280 0.23 -1.2 894,378 - RPSI07_RS03280 0.23 -1.3 894,378 - RPSI07_RS03280 0.23 +0.0 894,378 - RPSI07_RS03280 0.23 +1.4 894,378 - RPSI07_RS03280 0.23 -1.1 894,835 + -1.7 894,836 - +1.8 894,892 + +1.2 894,893 - -1.1 894,925 + -0.8 894,925 + -3.5 894,925 + +0.0 894,925 + -1.0 894,925 + +0.4 894,926 - -0.2 894,927 + +0.2 894,927 + -1.2 894,927 + +0.7 894,927 + -0.1 894,927 + +0.7 894,927 + -1.4 894,927 + +0.7 894,927 + -0.8 894,928 - +1.9 894,928 - -0.5 894,928 - -0.9 894,928 - -0.9 894,928 - -0.5 894,928 - +0.4 895,025 + +0.3 895,026 - +0.4 895,026 - -0.6 895,026 - -0.3 895,026 - -1.1 895,043 + -0.9 895,044 - -1.5 895,044 - -0.4 895,044 - -0.8 895,044 - +0.7 895,088 + +0.2 895,088 + +1.1 895,088 + -1.0 895,088 + -0.9 895,089 - +0.0 895,089 - +0.4 895,294 - RPSI07_RS03285 0.20 +0.8 895,390 - RPSI07_RS03285 0.28 +0.1 895,491 + RPSI07_RS03285 0.36 -1.0 895,491 + RPSI07_RS03285 0.36 +0.6 895,491 + RPSI07_RS03285 0.36 -1.4 895,491 + RPSI07_RS03285 0.36 +0.7 895,492 - RPSI07_RS03285 0.36 -2.6 895,556 + RPSI07_RS03285 0.41 -1.7 895,556 + RPSI07_RS03285 0.41 -0.5 895,557 + RPSI07_RS03285 0.41 +0.1 895,557 + RPSI07_RS03285 0.41 -2.1 895,558 - RPSI07_RS03285 0.41 -0.4 895,721 + RPSI07_RS03285 0.55 -1.2 895,722 - RPSI07_RS03285 0.55 -1.5 895,722 - RPSI07_RS03285 0.55 -0.5 895,722 - RPSI07_RS03285 0.55 +1.1 895,767 + RPSI07_RS03285 0.58 -0.3 895,770 + RPSI07_RS03285 0.59 -0.9 895,770 + RPSI07_RS03285 0.59 -0.1 895,770 + RPSI07_RS03285 0.59 -0.9 895,771 - RPSI07_RS03285 0.59 +0.7 895,797 + RPSI07_RS03285 0.61 -0.7 895,797 + RPSI07_RS03285 0.61 -0.8 895,797 + RPSI07_RS03285 0.61 -0.9 895,797 + RPSI07_RS03285 0.61 +0.8 895,798 - RPSI07_RS03285 0.61 -2.4 895,798 - RPSI07_RS03285 0.61 -0.5 895,798 - RPSI07_RS03285 0.61 +1.1 896,094 - RPSI07_RS03285 0.85 -0.7 896,165 - -0.6 896,165 - -0.2 896,165 - +0.7 896,165 - +1.5 896,165 - -0.5 896,165 - -0.5 896,165 - -0.7 896,176 - -0.1 896,214 + -1.2 896,214 + +1.0 896,214 + +0.1 896,214 + +0.1 896,214 + +0.4 896,215 - +2.9 896,215 - -0.1 896,215 - -1.3 896,225 + -0.5 896,225 + +0.0 896,225 + -0.2 896,226 - -0.4 896,226 - -1.1 896,296 + -0.2 896,296 + +1.3 896,296 + +0.2 896,297 - +2.1 896,492 + +0.2 896,493 - +0.5 896,637 + -1.0 897,039 - RPSI07_RS03290 0.39 -1.6
Or see this region's nucleotide sequence