Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS03025 and RPSI07_RS03030 are separated by 132 nucleotides RPSI07_RS03030 and RPSI07_RS03035 are separated by 185 nucleotides RPSI07_RS03035 and RPSI07_RS03040 are separated by 30 nucleotides
RPSI07_RS03025: RPSI07_RS03025 - glycoside hydrolase family 10 protein, at 831,915 to 833,258
_RS03025
RPSI07_RS03030: RPSI07_RS03030 - type VI secretion system protein TssA, at 833,391 to 834,467
_RS03030
RPSI07_RS03035: RPSI07_RS03035 - DUF393 domain-containing protein, at 834,653 to 835,105
_RS03035
RPSI07_RS03040: RPSI07_RS03040 - hypothetical protein, at 835,136 to 836,020
_RS03040
Position (kb) 833
834
835 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 832.498 kb on - strand, within RPSI07_RS03025 at 832.611 kb on + strand, within RPSI07_RS03025 at 832.611 kb on + strand, within RPSI07_RS03025 at 832.611 kb on + strand, within RPSI07_RS03025 at 832.612 kb on - strand, within RPSI07_RS03025 at 832.641 kb on + strand, within RPSI07_RS03025 at 832.641 kb on + strand, within RPSI07_RS03025 at 832.642 kb on - strand, within RPSI07_RS03025 at 832.642 kb on - strand, within RPSI07_RS03025 at 832.642 kb on - strand, within RPSI07_RS03025 at 832.642 kb on - strand, within RPSI07_RS03025 at 832.647 kb on + strand, within RPSI07_RS03025 at 832.650 kb on + strand, within RPSI07_RS03025 at 832.650 kb on + strand, within RPSI07_RS03025 at 832.650 kb on + strand, within RPSI07_RS03025 at 832.651 kb on - strand, within RPSI07_RS03025 at 832.651 kb on - strand, within RPSI07_RS03025 at 832.689 kb on + strand, within RPSI07_RS03025 at 832.689 kb on + strand, within RPSI07_RS03025 at 832.689 kb on + strand, within RPSI07_RS03025 at 832.689 kb on + strand, within RPSI07_RS03025 at 832.689 kb on + strand, within RPSI07_RS03025 at 832.690 kb on - strand, within RPSI07_RS03025 at 832.690 kb on - strand, within RPSI07_RS03025 at 832.690 kb on - strand, within RPSI07_RS03025 at 832.690 kb on - strand, within RPSI07_RS03025 at 832.690 kb on - strand, within RPSI07_RS03025 at 832.746 kb on + strand, within RPSI07_RS03025 at 832.746 kb on + strand, within RPSI07_RS03025 at 832.746 kb on + strand, within RPSI07_RS03025 at 832.746 kb on + strand, within RPSI07_RS03025 at 832.746 kb on + strand, within RPSI07_RS03025 at 832.747 kb on - strand, within RPSI07_RS03025 at 832.747 kb on - strand, within RPSI07_RS03025 at 832.747 kb on - strand, within RPSI07_RS03025 at 832.747 kb on - strand, within RPSI07_RS03025 at 832.747 kb on - strand, within RPSI07_RS03025 at 832.884 kb on + strand, within RPSI07_RS03025 at 832.884 kb on + strand, within RPSI07_RS03025 at 832.885 kb on - strand, within RPSI07_RS03025 at 832.885 kb on - strand, within RPSI07_RS03025 at 832.885 kb on - strand, within RPSI07_RS03025 at 832.896 kb on + strand, within RPSI07_RS03025 at 832.896 kb on + strand, within RPSI07_RS03025 at 832.897 kb on - strand, within RPSI07_RS03025 at 832.938 kb on + strand, within RPSI07_RS03025 at 832.939 kb on - strand, within RPSI07_RS03025 at 832.939 kb on - strand, within RPSI07_RS03025 at 832.956 kb on + strand, within RPSI07_RS03025 at 832.956 kb on + strand, within RPSI07_RS03025 at 832.956 kb on + strand, within RPSI07_RS03025 at 832.957 kb on - strand, within RPSI07_RS03025 at 832.957 kb on - strand, within RPSI07_RS03025 at 832.957 kb on - strand, within RPSI07_RS03025 at 832.987 kb on - strand, within RPSI07_RS03025 at 832.987 kb on - strand, within RPSI07_RS03025 at 833.043 kb on + strand, within RPSI07_RS03025 at 833.043 kb on + strand, within RPSI07_RS03025 at 833.043 kb on + strand, within RPSI07_RS03025 at 833.043 kb on + strand, within RPSI07_RS03025 at 833.043 kb on + strand, within RPSI07_RS03025 at 833.043 kb on + strand, within RPSI07_RS03025 at 833.044 kb on - strand, within RPSI07_RS03025 at 833.061 kb on + strand, within RPSI07_RS03025 at 833.062 kb on - strand, within RPSI07_RS03025 at 833.062 kb on - strand, within RPSI07_RS03025 at 833.230 kb on - strand at 833.312 kb on + strand at 833.313 kb on - strand at 833.682 kb on + strand, within RPSI07_RS03030 at 833.683 kb on - strand, within RPSI07_RS03030 at 833.712 kb on + strand, within RPSI07_RS03030 at 833.713 kb on - strand, within RPSI07_RS03030 at 833.850 kb on + strand, within RPSI07_RS03030 at 833.850 kb on + strand, within RPSI07_RS03030 at 833.850 kb on + strand, within RPSI07_RS03030 at 833.850 kb on + strand, within RPSI07_RS03030 at 833.850 kb on + strand, within RPSI07_RS03030 at 833.851 kb on - strand, within RPSI07_RS03030 at 833.851 kb on - strand, within RPSI07_RS03030 at 833.851 kb on - strand, within RPSI07_RS03030 at 833.851 kb on - strand, within RPSI07_RS03030 at 833.985 kb on + strand, within RPSI07_RS03030 at 833.985 kb on + strand, within RPSI07_RS03030 at 833.985 kb on + strand, within RPSI07_RS03030 at 833.985 kb on + strand, within RPSI07_RS03030 at 833.986 kb on - strand, within RPSI07_RS03030 at 833.986 kb on - strand, within RPSI07_RS03030 at 833.987 kb on + strand, within RPSI07_RS03030 at 833.987 kb on + strand, within RPSI07_RS03030 at 833.988 kb on - strand, within RPSI07_RS03030 at 833.988 kb on - strand, within RPSI07_RS03030 at 833.997 kb on + strand, within RPSI07_RS03030 at 833.997 kb on + strand, within RPSI07_RS03030 at 833.997 kb on + strand, within RPSI07_RS03030 at 833.998 kb on - strand, within RPSI07_RS03030 at 834.327 kb on + strand, within RPSI07_RS03030 at 834.327 kb on + strand, within RPSI07_RS03030 at 834.327 kb on + strand, within RPSI07_RS03030 at 834.328 kb on - strand, within RPSI07_RS03030 at 834.517 kb on + strand at 834.556 kb on + strand at 834.556 kb on + strand at 834.556 kb on + strand at 834.556 kb on + strand at 834.556 kb on + strand at 834.557 kb on - strand at 834.557 kb on - strand at 834.577 kb on + strand at 834.626 kb on + strand at 834.627 kb on - strand at 834.747 kb on + strand, within RPSI07_RS03035 at 834.873 kb on + strand, within RPSI07_RS03035 at 834.873 kb on + strand, within RPSI07_RS03035 at 834.873 kb on + strand, within RPSI07_RS03035 at 834.874 kb on - strand, within RPSI07_RS03035 at 834.874 kb on - strand, within RPSI07_RS03035 at 834.909 kb on + strand, within RPSI07_RS03035 at 834.910 kb on - strand, within RPSI07_RS03035 at 834.917 kb on + strand, within RPSI07_RS03035 at 834.917 kb on + strand, within RPSI07_RS03035 at 834.925 kb on + strand, within RPSI07_RS03035 at 834.926 kb on - strand, within RPSI07_RS03035 at 834.926 kb on - strand, within RPSI07_RS03035 at 835.203 kb on + strand at 835.203 kb on + strand at 835.204 kb on - strand at 835.398 kb on + strand, within RPSI07_RS03040 at 835.398 kb on + strand, within RPSI07_RS03040 at 835.398 kb on + strand, within RPSI07_RS03040 at 835.398 kb on + strand, within RPSI07_RS03040 at 835.399 kb on - strand, within RPSI07_RS03040 at 835.399 kb on - strand, within RPSI07_RS03040 at 835.411 kb on + strand, within RPSI07_RS03040 at 835.411 kb on + strand, within RPSI07_RS03040 at 835.412 kb on - strand, within RPSI07_RS03040 at 835.412 kb on - strand, within RPSI07_RS03040 at 835.419 kb on + strand, within RPSI07_RS03040 at 835.419 kb on + strand, within RPSI07_RS03040 at 835.419 kb on + strand, within RPSI07_RS03040 at 835.419 kb on + strand, within RPSI07_RS03040 at 835.420 kb on - strand, within RPSI07_RS03040 at 835.420 kb on - strand, within RPSI07_RS03040 at 835.420 kb on - strand, within RPSI07_RS03040 at 835.422 kb on + strand, within RPSI07_RS03040 at 835.422 kb on + strand, within RPSI07_RS03040 at 835.423 kb on - strand, within RPSI07_RS03040 at 835.423 kb on - strand, within RPSI07_RS03040 at 835.423 kb on - strand, within RPSI07_RS03040 at 835.423 kb on - strand, within RPSI07_RS03040 at 835.467 kb on + strand, within RPSI07_RS03040 at 835.467 kb on + strand, within RPSI07_RS03040 at 835.467 kb on + strand, within RPSI07_RS03040 at 835.467 kb on + strand, within RPSI07_RS03040 at 835.467 kb on + strand, within RPSI07_RS03040 at 835.467 kb on + strand, within RPSI07_RS03040 at 835.467 kb on + strand, within RPSI07_RS03040 at 835.467 kb on + strand, within RPSI07_RS03040 at 835.467 kb on + strand, within RPSI07_RS03040
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 832,498 - RPSI07_RS03025 0.43 -0.5 832,611 + RPSI07_RS03025 0.52 +1.4 832,611 + RPSI07_RS03025 0.52 +0.5 832,611 + RPSI07_RS03025 0.52 +0.0 832,612 - RPSI07_RS03025 0.52 +0.7 832,641 + RPSI07_RS03025 0.54 +0.0 832,641 + RPSI07_RS03025 0.54 +0.1 832,642 - RPSI07_RS03025 0.54 -2.2 832,642 - RPSI07_RS03025 0.54 -0.6 832,642 - RPSI07_RS03025 0.54 +1.3 832,642 - RPSI07_RS03025 0.54 -0.5 832,647 + RPSI07_RS03025 0.54 +0.5 832,650 + RPSI07_RS03025 0.55 +1.7 832,650 + RPSI07_RS03025 0.55 +0.4 832,650 + RPSI07_RS03025 0.55 +2.7 832,651 - RPSI07_RS03025 0.55 -1.7 832,651 - RPSI07_RS03025 0.55 +0.7 832,689 + RPSI07_RS03025 0.58 +0.7 832,689 + RPSI07_RS03025 0.58 +0.3 832,689 + RPSI07_RS03025 0.58 -0.6 832,689 + RPSI07_RS03025 0.58 -0.3 832,689 + RPSI07_RS03025 0.58 -0.6 832,690 - RPSI07_RS03025 0.58 -1.6 832,690 - RPSI07_RS03025 0.58 -0.8 832,690 - RPSI07_RS03025 0.58 -0.8 832,690 - RPSI07_RS03025 0.58 +0.0 832,690 - RPSI07_RS03025 0.58 -1.8 832,746 + RPSI07_RS03025 0.62 +0.2 832,746 + RPSI07_RS03025 0.62 +0.0 832,746 + RPSI07_RS03025 0.62 -0.1 832,746 + RPSI07_RS03025 0.62 -0.0 832,746 + RPSI07_RS03025 0.62 -0.1 832,747 - RPSI07_RS03025 0.62 -0.7 832,747 - RPSI07_RS03025 0.62 -0.9 832,747 - RPSI07_RS03025 0.62 -0.8 832,747 - RPSI07_RS03025 0.62 +0.4 832,747 - RPSI07_RS03025 0.62 -0.3 832,884 + RPSI07_RS03025 0.72 +0.3 832,884 + RPSI07_RS03025 0.72 +0.5 832,885 - RPSI07_RS03025 0.72 +0.7 832,885 - RPSI07_RS03025 0.72 -0.3 832,885 - RPSI07_RS03025 0.72 +0.2 832,896 + RPSI07_RS03025 0.73 +0.1 832,896 + RPSI07_RS03025 0.73 +3.6 832,897 - RPSI07_RS03025 0.73 +0.2 832,938 + RPSI07_RS03025 0.76 +0.9 832,939 - RPSI07_RS03025 0.76 -0.7 832,939 - RPSI07_RS03025 0.76 +0.2 832,956 + RPSI07_RS03025 0.77 -0.1 832,956 + RPSI07_RS03025 0.77 -1.3 832,956 + RPSI07_RS03025 0.77 +0.7 832,957 - RPSI07_RS03025 0.78 -1.6 832,957 - RPSI07_RS03025 0.78 +0.6 832,957 - RPSI07_RS03025 0.78 +0.4 832,987 - RPSI07_RS03025 0.80 -0.8 832,987 - RPSI07_RS03025 0.80 -0.1 833,043 + RPSI07_RS03025 0.84 -0.3 833,043 + RPSI07_RS03025 0.84 -0.5 833,043 + RPSI07_RS03025 0.84 -0.3 833,043 + RPSI07_RS03025 0.84 +1.2 833,043 + RPSI07_RS03025 0.84 +0.3 833,043 + RPSI07_RS03025 0.84 +2.3 833,044 - RPSI07_RS03025 0.84 +1.0 833,061 + RPSI07_RS03025 0.85 -0.1 833,062 - RPSI07_RS03025 0.85 +0.7 833,062 - RPSI07_RS03025 0.85 +0.7 833,230 - +1.4 833,312 + +0.6 833,313 - +0.5 833,682 + RPSI07_RS03030 0.27 -2.1 833,683 - RPSI07_RS03030 0.27 -0.1 833,712 + RPSI07_RS03030 0.30 -0.8 833,713 - RPSI07_RS03030 0.30 +0.0 833,850 + RPSI07_RS03030 0.43 -2.4 833,850 + RPSI07_RS03030 0.43 -0.6 833,850 + RPSI07_RS03030 0.43 +1.3 833,850 + RPSI07_RS03030 0.43 -0.9 833,850 + RPSI07_RS03030 0.43 +0.7 833,851 - RPSI07_RS03030 0.43 -0.3 833,851 - RPSI07_RS03030 0.43 -1.1 833,851 - RPSI07_RS03030 0.43 -1.1 833,851 - RPSI07_RS03030 0.43 +0.7 833,985 + RPSI07_RS03030 0.55 -1.8 833,985 + RPSI07_RS03030 0.55 -2.0 833,985 + RPSI07_RS03030 0.55 -2.0 833,985 + RPSI07_RS03030 0.55 +1.2 833,986 - RPSI07_RS03030 0.55 -0.5 833,986 - RPSI07_RS03030 0.55 -3.1 833,987 + RPSI07_RS03030 0.55 -0.8 833,987 + RPSI07_RS03030 0.55 -3.4 833,988 - RPSI07_RS03030 0.55 -0.6 833,988 - RPSI07_RS03030 0.55 -2.8 833,997 + RPSI07_RS03030 0.56 -0.6 833,997 + RPSI07_RS03030 0.56 +0.7 833,997 + RPSI07_RS03030 0.56 -1.6 833,998 - RPSI07_RS03030 0.56 +1.7 834,327 + RPSI07_RS03030 0.87 -0.5 834,327 + RPSI07_RS03030 0.87 -0.0 834,327 + RPSI07_RS03030 0.87 +0.4 834,328 - RPSI07_RS03030 0.87 -1.4 834,517 + -0.1 834,556 + +0.4 834,556 + -0.5 834,556 + +0.0 834,556 + +2.3 834,556 + +0.4 834,557 - -0.3 834,557 - -0.1 834,577 + +1.3 834,626 + +0.7 834,627 - -1.9 834,747 + RPSI07_RS03035 0.21 +1.7 834,873 + RPSI07_RS03035 0.49 +2.7 834,873 + RPSI07_RS03035 0.49 -0.3 834,873 + RPSI07_RS03035 0.49 +0.7 834,874 - RPSI07_RS03035 0.49 -1.3 834,874 - RPSI07_RS03035 0.49 -0.6 834,909 + RPSI07_RS03035 0.57 -0.8 834,910 - RPSI07_RS03035 0.57 -0.8 834,917 + RPSI07_RS03035 0.58 -3.0 834,917 + RPSI07_RS03035 0.58 -1.2 834,925 + RPSI07_RS03035 0.60 -0.8 834,926 - RPSI07_RS03035 0.60 -3.3 834,926 - RPSI07_RS03035 0.60 +3.1 835,203 + -0.3 835,203 + -0.1 835,204 - +0.8 835,398 + RPSI07_RS03040 0.30 -0.3 835,398 + RPSI07_RS03040 0.30 +0.4 835,398 + RPSI07_RS03040 0.30 +1.0 835,398 + RPSI07_RS03040 0.30 -3.7 835,399 - RPSI07_RS03040 0.30 -0.4 835,399 - RPSI07_RS03040 0.30 +0.4 835,411 + RPSI07_RS03040 0.31 +0.7 835,411 + RPSI07_RS03040 0.31 +0.1 835,412 - RPSI07_RS03040 0.31 -0.2 835,412 - RPSI07_RS03040 0.31 +0.9 835,419 + RPSI07_RS03040 0.32 -0.7 835,419 + RPSI07_RS03040 0.32 -0.9 835,419 + RPSI07_RS03040 0.32 +0.3 835,419 + RPSI07_RS03040 0.32 -0.4 835,420 - RPSI07_RS03040 0.32 +1.0 835,420 - RPSI07_RS03040 0.32 +1.7 835,420 - RPSI07_RS03040 0.32 +1.2 835,422 + RPSI07_RS03040 0.32 +1.6 835,422 + RPSI07_RS03040 0.32 +0.7 835,423 - RPSI07_RS03040 0.32 -1.3 835,423 - RPSI07_RS03040 0.32 -0.1 835,423 - RPSI07_RS03040 0.32 -0.4 835,423 - RPSI07_RS03040 0.32 +1.7 835,467 + RPSI07_RS03040 0.37 -1.0 835,467 + RPSI07_RS03040 0.37 -0.9 835,467 + RPSI07_RS03040 0.37 +0.1 835,467 + RPSI07_RS03040 0.37 -1.6 835,467 + RPSI07_RS03040 0.37 -1.9 835,467 + RPSI07_RS03040 0.37 -0.1 835,467 + RPSI07_RS03040 0.37 +0.6 835,467 + RPSI07_RS03040 0.37 +1.7 835,467 + RPSI07_RS03040 0.37 +0.4
Or see this region's nucleotide sequence