Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS01305 and RPSI07_RS01310 are separated by 0 nucleotides RPSI07_RS01310 and RPSI07_RS01315 are separated by 41 nucleotides RPSI07_RS01315 and RPSI07_RS01320 are separated by 127 nucleotides
RPSI07_RS01305: RPSI07_RS01305 - heavy metal translocating P-type ATPase, at 359,997 to 362,351
_RS01305
RPSI07_RS01310: RPSI07_RS01310 - Cd(II)/Pb(II)-responsive transcriptional regulator, at 362,352 to 362,828
_RS01310
RPSI07_RS01315: RPSI07_RS01315 - hypothetical protein, at 362,870 to 363,271
_RS01315
RPSI07_RS01320: RPSI07_RS01320 - 2-isopropylmalate synthase, at 363,399 to 365,129
_RS01320
Position (kb) 362
363 Strain fitness (log2 ratio) -2
-1
0
1
2 at 361.453 kb on + strand, within RPSI07_RS01305 at 361.453 kb on + strand, within RPSI07_RS01305 at 361.454 kb on - strand, within RPSI07_RS01305 at 361.454 kb on - strand, within RPSI07_RS01305 at 361.454 kb on - strand, within RPSI07_RS01305 at 361.538 kb on + strand, within RPSI07_RS01305 at 361.539 kb on - strand, within RPSI07_RS01305 at 361.712 kb on + strand, within RPSI07_RS01305 at 361.712 kb on + strand, within RPSI07_RS01305 at 361.712 kb on + strand, within RPSI07_RS01305 at 361.713 kb on - strand, within RPSI07_RS01305 at 361.713 kb on - strand, within RPSI07_RS01305 at 361.713 kb on - strand, within RPSI07_RS01305 at 361.713 kb on - strand, within RPSI07_RS01305 at 361.769 kb on + strand, within RPSI07_RS01305 at 362.004 kb on + strand, within RPSI07_RS01305 at 362.005 kb on - strand, within RPSI07_RS01305 at 362.006 kb on - strand, within RPSI07_RS01305 at 362.349 kb on + strand at 362.349 kb on + strand at 362.349 kb on + strand at 362.349 kb on + strand at 362.350 kb on - strand at 362.350 kb on - strand at 362.350 kb on - strand at 362.353 kb on + strand at 362.353 kb on + strand at 362.354 kb on - strand at 362.372 kb on + strand at 362.714 kb on - strand, within RPSI07_RS01310 at 362.722 kb on + strand, within RPSI07_RS01310 at 362.723 kb on - strand, within RPSI07_RS01310 at 362.723 kb on - strand, within RPSI07_RS01310 at 362.723 kb on - strand, within RPSI07_RS01310 at 362.770 kb on + strand, within RPSI07_RS01310 at 362.864 kb on + strand at 362.864 kb on + strand at 362.864 kb on + strand at 362.864 kb on + strand at 362.865 kb on - strand at 362.865 kb on - strand at 362.865 kb on - strand at 362.865 kb on - strand at 362.870 kb on + strand at 362.872 kb on - strand at 362.872 kb on - strand at 363.119 kb on - strand, within RPSI07_RS01315 at 363.185 kb on + strand, within RPSI07_RS01315 at 363.185 kb on + strand, within RPSI07_RS01315 at 363.185 kb on + strand, within RPSI07_RS01315 at 363.185 kb on + strand, within RPSI07_RS01315 at 363.185 kb on + strand, within RPSI07_RS01315 at 363.186 kb on - strand, within RPSI07_RS01315 at 363.360 kb on + strand at 363.360 kb on + strand at 363.360 kb on + strand at 363.360 kb on + strand at 363.360 kb on + strand at 363.360 kb on + strand at 363.361 kb on - strand at 363.361 kb on - strand at 363.361 kb on - strand at 363.375 kb on + strand at 363.376 kb on - strand at 363.376 kb on - strand at 363.387 kb on - strand at 363.635 kb on - strand, within RPSI07_RS01320 at 363.635 kb on - strand, within RPSI07_RS01320
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 361,453 + RPSI07_RS01305 0.62 -0.4 361,453 + RPSI07_RS01305 0.62 +0.7 361,454 - RPSI07_RS01305 0.62 +2.7 361,454 - RPSI07_RS01305 0.62 +0.9 361,454 - RPSI07_RS01305 0.62 -0.3 361,538 + RPSI07_RS01305 0.65 +1.3 361,539 - RPSI07_RS01305 0.65 +0.2 361,712 + RPSI07_RS01305 0.73 -0.7 361,712 + RPSI07_RS01305 0.73 -0.9 361,712 + RPSI07_RS01305 0.73 +0.3 361,713 - RPSI07_RS01305 0.73 -0.9 361,713 - RPSI07_RS01305 0.73 -1.0 361,713 - RPSI07_RS01305 0.73 -1.1 361,713 - RPSI07_RS01305 0.73 -0.6 361,769 + RPSI07_RS01305 0.75 +1.5 362,004 + RPSI07_RS01305 0.85 -0.5 362,005 - RPSI07_RS01305 0.85 +0.5 362,006 - RPSI07_RS01305 0.85 +0.7 362,349 + +0.0 362,349 + +0.4 362,349 + -2.6 362,349 + -1.5 362,350 - -1.3 362,350 - -1.7 362,350 - +2.1 362,353 + -0.1 362,353 + +1.6 362,354 - -2.3 362,372 + -0.3 362,714 - RPSI07_RS01310 0.76 +2.5 362,722 + RPSI07_RS01310 0.78 -0.0 362,723 - RPSI07_RS01310 0.78 -0.2 362,723 - RPSI07_RS01310 0.78 -0.3 362,723 - RPSI07_RS01310 0.78 +0.3 362,770 + RPSI07_RS01310 0.88 +1.4 362,864 + -0.1 362,864 + -0.5 362,864 + +0.3 362,864 + -0.7 362,865 - +0.7 362,865 - +1.7 362,865 - -0.4 362,865 - +1.2 362,870 + +0.7 362,872 - -1.6 362,872 - +0.7 363,119 - RPSI07_RS01315 0.62 -1.3 363,185 + RPSI07_RS01315 0.78 -0.8 363,185 + RPSI07_RS01315 0.78 -0.3 363,185 + RPSI07_RS01315 0.78 -1.6 363,185 + RPSI07_RS01315 0.78 +0.2 363,185 + RPSI07_RS01315 0.78 -0.9 363,186 - RPSI07_RS01315 0.79 -0.3 363,360 + -0.3 363,360 + -0.2 363,360 + -1.6 363,360 + -0.9 363,360 + +0.7 363,360 + -0.7 363,361 - -0.7 363,361 - +0.1 363,361 - -0.8 363,375 + -0.0 363,376 - +0.4 363,376 - -0.2 363,387 - +1.7 363,635 - RPSI07_RS01320 0.14 +0.7 363,635 - RPSI07_RS01320 0.14 -0.2
Or see this region's nucleotide sequence