Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS00755 and RPSI07_RS00760 are separated by 123 nucleotides RPSI07_RS00760 and RPSI07_RS00765 are separated by 11 nucleotides
RPSI07_RS00755: RPSI07_RS00755 - LysR family transcriptional regulator, at 202,793 to 203,707
_RS00755
RPSI07_RS00760: RPSI07_RS00760 - aromatic ring-hydroxylating dioxygenase subunit alpha, at 203,831 to 204,859
_RS00760
RPSI07_RS00765: RPSI07_RS00765 - oxidoreductase, at 204,871 to 205,815
_RS00765
Position (kb) 203
204
205 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 203.212 kb on + strand, within RPSI07_RS00755 at 203.212 kb on + strand, within RPSI07_RS00755 at 203.212 kb on + strand, within RPSI07_RS00755 at 203.212 kb on + strand, within RPSI07_RS00755 at 203.212 kb on + strand, within RPSI07_RS00755 at 203.212 kb on + strand, within RPSI07_RS00755 at 203.212 kb on + strand, within RPSI07_RS00755 at 203.212 kb on + strand, within RPSI07_RS00755 at 203.212 kb on + strand, within RPSI07_RS00755 at 203.213 kb on - strand, within RPSI07_RS00755 at 203.213 kb on - strand, within RPSI07_RS00755 at 203.213 kb on - strand, within RPSI07_RS00755 at 203.213 kb on - strand, within RPSI07_RS00755 at 203.213 kb on - strand, within RPSI07_RS00755 at 203.213 kb on - strand, within RPSI07_RS00755 at 203.213 kb on - strand, within RPSI07_RS00755 at 203.213 kb on - strand, within RPSI07_RS00755 at 203.213 kb on - strand, within RPSI07_RS00755 at 203.213 kb on - strand, within RPSI07_RS00755 at 203.213 kb on - strand, within RPSI07_RS00755 at 203.213 kb on - strand, within RPSI07_RS00755 at 203.213 kb on - strand, within RPSI07_RS00755 at 203.215 kb on - strand, within RPSI07_RS00755 at 203.514 kb on + strand, within RPSI07_RS00755 at 203.514 kb on + strand, within RPSI07_RS00755 at 203.514 kb on + strand, within RPSI07_RS00755 at 203.515 kb on - strand, within RPSI07_RS00755 at 203.515 kb on - strand, within RPSI07_RS00755 at 203.655 kb on + strand at 203.655 kb on + strand at 203.655 kb on + strand at 203.655 kb on + strand at 203.655 kb on + strand at 203.656 kb on - strand at 203.656 kb on - strand at 203.656 kb on - strand at 203.656 kb on - strand at 203.656 kb on - strand at 203.656 kb on - strand at 203.742 kb on - strand at 203.852 kb on + strand at 203.852 kb on + strand at 203.852 kb on + strand at 203.852 kb on + strand at 203.852 kb on + strand at 203.853 kb on - strand at 203.853 kb on - strand at 203.853 kb on - strand at 203.853 kb on - strand at 203.853 kb on - strand at 203.853 kb on - strand at 203.853 kb on - strand at 203.853 kb on - strand at 203.856 kb on + strand at 203.856 kb on + strand at 203.856 kb on + strand at 203.856 kb on + strand at 203.856 kb on + strand at 203.856 kb on + strand at 203.856 kb on + strand at 203.857 kb on - strand at 203.857 kb on - strand at 203.857 kb on - strand at 203.857 kb on - strand at 203.857 kb on - strand at 203.857 kb on - strand at 203.857 kb on - strand at 203.857 kb on - strand at 203.857 kb on - strand at 203.857 kb on - strand at 203.857 kb on - strand at 203.857 kb on - strand at 203.857 kb on - strand at 203.858 kb on - strand at 203.927 kb on + strand at 203.927 kb on + strand at 203.927 kb on + strand at 203.927 kb on + strand at 203.927 kb on + strand at 203.927 kb on + strand at 203.927 kb on + strand at 203.928 kb on - strand at 203.928 kb on - strand at 203.928 kb on - strand at 204.032 kb on + strand, within RPSI07_RS00760 at 204.228 kb on - strand, within RPSI07_RS00760 at 204.242 kb on + strand, within RPSI07_RS00760 at 204.242 kb on + strand, within RPSI07_RS00760 at 204.243 kb on - strand, within RPSI07_RS00760 at 204.243 kb on - strand, within RPSI07_RS00760 at 204.243 kb on - strand, within RPSI07_RS00760 at 204.305 kb on + strand, within RPSI07_RS00760 at 204.306 kb on - strand, within RPSI07_RS00760 at 204.306 kb on - strand, within RPSI07_RS00760 at 204.306 kb on - strand, within RPSI07_RS00760 at 204.555 kb on - strand, within RPSI07_RS00760 at 204.586 kb on + strand, within RPSI07_RS00760 at 204.586 kb on + strand, within RPSI07_RS00760 at 204.587 kb on - strand, within RPSI07_RS00760 at 204.587 kb on - strand, within RPSI07_RS00760 at 204.632 kb on + strand, within RPSI07_RS00760 at 204.633 kb on - strand, within RPSI07_RS00760 at 204.633 kb on - strand, within RPSI07_RS00760 at 204.633 kb on - strand, within RPSI07_RS00760 at 204.633 kb on - strand, within RPSI07_RS00760 at 204.764 kb on + strand at 204.765 kb on - strand at 204.857 kb on + strand at 204.858 kb on - strand at 205.012 kb on + strand, within RPSI07_RS00765 at 205.013 kb on - strand, within RPSI07_RS00765 at 205.270 kb on + strand, within RPSI07_RS00765 at 205.271 kb on - strand, within RPSI07_RS00765 at 205.271 kb on - strand, within RPSI07_RS00765 at 205.271 kb on - strand, within RPSI07_RS00765 at 205.348 kb on + strand, within RPSI07_RS00765 at 205.348 kb on + strand, within RPSI07_RS00765 at 205.349 kb on - strand, within RPSI07_RS00765 at 205.349 kb on - strand, within RPSI07_RS00765 at 205.349 kb on - strand, within RPSI07_RS00765 at 205.349 kb on - strand, within RPSI07_RS00765 at 205.426 kb on + strand, within RPSI07_RS00765 at 205.426 kb on + strand, within RPSI07_RS00765 at 205.426 kb on + strand, within RPSI07_RS00765 at 205.426 kb on + strand, within RPSI07_RS00765 at 205.426 kb on + strand, within RPSI07_RS00765 at 205.426 kb on + strand, within RPSI07_RS00765 at 205.427 kb on - strand, within RPSI07_RS00765 at 205.427 kb on - strand, within RPSI07_RS00765 at 205.427 kb on - strand, within RPSI07_RS00765 at 205.427 kb on - strand, within RPSI07_RS00765 at 205.427 kb on - strand, within RPSI07_RS00765 at 205.513 kb on + strand, within RPSI07_RS00765 at 205.514 kb on - strand, within RPSI07_RS00765 at 205.732 kb on + strand at 205.732 kb on + strand at 205.733 kb on - strand at 205.733 kb on - strand at 205.813 kb on + strand at 205.813 kb on + strand at 205.813 kb on + strand at 205.813 kb on + strand at 205.813 kb on + strand at 205.813 kb on + strand at 205.814 kb on - strand at 205.814 kb on - strand at 205.814 kb on - strand at 205.814 kb on - strand at 205.814 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 203,212 + RPSI07_RS00755 0.46 +0.7 203,212 + RPSI07_RS00755 0.46 -0.4 203,212 + RPSI07_RS00755 0.46 -0.1 203,212 + RPSI07_RS00755 0.46 +0.2 203,212 + RPSI07_RS00755 0.46 +1.2 203,212 + RPSI07_RS00755 0.46 -0.2 203,212 + RPSI07_RS00755 0.46 -1.1 203,212 + RPSI07_RS00755 0.46 +0.6 203,212 + RPSI07_RS00755 0.46 -1.7 203,213 - RPSI07_RS00755 0.46 +1.2 203,213 - RPSI07_RS00755 0.46 +0.7 203,213 - RPSI07_RS00755 0.46 -0.5 203,213 - RPSI07_RS00755 0.46 -1.6 203,213 - RPSI07_RS00755 0.46 +1.0 203,213 - RPSI07_RS00755 0.46 +0.7 203,213 - RPSI07_RS00755 0.46 -1.4 203,213 - RPSI07_RS00755 0.46 -1.4 203,213 - RPSI07_RS00755 0.46 +0.2 203,213 - RPSI07_RS00755 0.46 -0.6 203,213 - RPSI07_RS00755 0.46 -1.1 203,213 - RPSI07_RS00755 0.46 -0.2 203,213 - RPSI07_RS00755 0.46 -1.5 203,215 - RPSI07_RS00755 0.46 +2.7 203,514 + RPSI07_RS00755 0.79 -1.0 203,514 + RPSI07_RS00755 0.79 +0.3 203,514 + RPSI07_RS00755 0.79 -0.3 203,515 - RPSI07_RS00755 0.79 +2.9 203,515 - RPSI07_RS00755 0.79 -0.1 203,655 + -0.1 203,655 + -1.2 203,655 + +1.0 203,655 + +0.3 203,655 + -0.3 203,656 - -0.0 203,656 - -1.0 203,656 - -1.0 203,656 - -1.5 203,656 - +0.2 203,656 - -0.3 203,742 - -1.6 203,852 + -1.4 203,852 + +1.2 203,852 + +0.9 203,852 + +0.0 203,852 + -0.3 203,853 - +1.7 203,853 - -0.8 203,853 - +1.2 203,853 - -0.9 203,853 - +1.0 203,853 - +1.7 203,853 - -0.3 203,853 - +0.9 203,856 + -0.0 203,856 + +0.4 203,856 + -1.8 203,856 + -0.3 203,856 + +0.2 203,856 + -0.1 203,856 + +1.0 203,857 - -0.1 203,857 - -1.8 203,857 - -0.6 203,857 - -2.6 203,857 - -0.9 203,857 - -0.1 203,857 - +0.5 203,857 - -0.8 203,857 - -0.0 203,857 - -0.3 203,857 - -1.8 203,857 - -0.6 203,857 - +0.2 203,858 - +1.4 203,927 + +0.8 203,927 + -1.0 203,927 + -1.7 203,927 + +0.2 203,927 + +0.2 203,927 + +1.1 203,927 + +0.4 203,928 - +0.2 203,928 - +3.5 203,928 - -2.2 204,032 + RPSI07_RS00760 0.20 +0.7 204,228 - RPSI07_RS00760 0.39 -0.5 204,242 + RPSI07_RS00760 0.40 -0.6 204,242 + RPSI07_RS00760 0.40 +1.5 204,243 - RPSI07_RS00760 0.40 -0.5 204,243 - RPSI07_RS00760 0.40 +2.1 204,243 - RPSI07_RS00760 0.40 +0.5 204,305 + RPSI07_RS00760 0.46 -1.9 204,306 - RPSI07_RS00760 0.46 +0.5 204,306 - RPSI07_RS00760 0.46 -0.9 204,306 - RPSI07_RS00760 0.46 -1.1 204,555 - RPSI07_RS00760 0.70 +0.3 204,586 + RPSI07_RS00760 0.73 +1.1 204,586 + RPSI07_RS00760 0.73 -0.8 204,587 - RPSI07_RS00760 0.73 +0.7 204,587 - RPSI07_RS00760 0.73 +1.6 204,632 + RPSI07_RS00760 0.78 +0.5 204,633 - RPSI07_RS00760 0.78 -2.3 204,633 - RPSI07_RS00760 0.78 +1.3 204,633 - RPSI07_RS00760 0.78 -0.6 204,633 - RPSI07_RS00760 0.78 +0.7 204,764 + +1.4 204,765 - +0.4 204,857 + +1.5 204,858 - +1.1 205,012 + RPSI07_RS00765 0.15 +0.7 205,013 - RPSI07_RS00765 0.15 +0.9 205,270 + RPSI07_RS00765 0.42 +1.1 205,271 - RPSI07_RS00765 0.42 -0.2 205,271 - RPSI07_RS00765 0.42 -1.7 205,271 - RPSI07_RS00765 0.42 +1.3 205,348 + RPSI07_RS00765 0.50 -1.2 205,348 + RPSI07_RS00765 0.50 +2.2 205,349 - RPSI07_RS00765 0.51 +1.0 205,349 - RPSI07_RS00765 0.51 -0.6 205,349 - RPSI07_RS00765 0.51 +0.4 205,349 - RPSI07_RS00765 0.51 +1.5 205,426 + RPSI07_RS00765 0.59 +1.7 205,426 + RPSI07_RS00765 0.59 +1.9 205,426 + RPSI07_RS00765 0.59 +0.4 205,426 + RPSI07_RS00765 0.59 +0.1 205,426 + RPSI07_RS00765 0.59 +0.2 205,426 + RPSI07_RS00765 0.59 +0.8 205,427 - RPSI07_RS00765 0.59 -0.1 205,427 - RPSI07_RS00765 0.59 +0.4 205,427 - RPSI07_RS00765 0.59 -0.8 205,427 - RPSI07_RS00765 0.59 +0.7 205,427 - RPSI07_RS00765 0.59 -0.5 205,513 + RPSI07_RS00765 0.68 +0.9 205,514 - RPSI07_RS00765 0.68 +1.5 205,732 + +0.7 205,732 + +1.9 205,733 - +3.3 205,733 - -1.0 205,813 + +0.2 205,813 + +0.1 205,813 + +1.7 205,813 + -0.1 205,813 + +0.5 205,813 + +0.7 205,814 - +0.7 205,814 - +0.7 205,814 - +0.2 205,814 - +1.7 205,814 - +0.6
Or see this region's nucleotide sequence