Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS22275 and RPSI07_RS22280 are separated by 10 nucleotides RPSI07_RS22280 and RPSI07_RS22285 are separated by 58 nucleotides RPSI07_RS22285 and RPSI07_RS22290 are separated by 10 nucleotides RPSI07_RS22290 and RPSI07_RS22295 are separated by 10 nucleotides
RPSI07_RS22275: RPSI07_RS22275 - nitrate ABC transporter, permease protein, at 3,161,636 to 3,162,487
_RS22275
RPSI07_RS22280: RPSI07_RS22280 - ABC transporter ATP-binding protein, at 3,162,498 to 3,163,304
_RS22280
RPSI07_RS22285: RPSI07_RS22285 - DNA-binding protein YbiB, at 3,163,363 to 3,164,376
_RS22285
RPSI07_RS22290: RPSI07_RS22290 - pirin family protein, at 3,164,387 to 3,165,256
_RS22290
RPSI07_RS22295: RPSI07_RS22295 - OsmC family peroxiredoxin, at 3,165,267 to 3,165,671
_RS22295
Position (kb)
3163
3164
3165 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3162.795 kb on + strand, within RPSI07_RS22280 at 3162.795 kb on + strand, within RPSI07_RS22280 at 3162.796 kb on - strand, within RPSI07_RS22280 at 3163.041 kb on - strand, within RPSI07_RS22280 at 3163.227 kb on + strand at 3163.228 kb on - strand at 3163.302 kb on + strand at 3163.327 kb on - strand at 3163.333 kb on + strand at 3163.333 kb on + strand at 3163.333 kb on + strand at 3163.333 kb on + strand at 3163.333 kb on + strand at 3163.333 kb on + strand at 3163.333 kb on + strand at 3163.334 kb on - strand at 3163.334 kb on - strand at 3163.334 kb on - strand at 3163.334 kb on - strand at 3163.334 kb on - strand at 3163.334 kb on - strand at 3163.334 kb on - strand at 3163.334 kb on - strand at 3163.334 kb on - strand at 3163.690 kb on + strand, within RPSI07_RS22285 at 3163.690 kb on + strand, within RPSI07_RS22285 at 3163.690 kb on + strand, within RPSI07_RS22285 at 3163.690 kb on + strand, within RPSI07_RS22285 at 3163.691 kb on - strand, within RPSI07_RS22285 at 3164.041 kb on + strand, within RPSI07_RS22285 at 3164.041 kb on + strand, within RPSI07_RS22285 at 3164.042 kb on - strand, within RPSI07_RS22285 at 3164.042 kb on - strand, within RPSI07_RS22285 at 3164.042 kb on - strand, within RPSI07_RS22285 at 3164.077 kb on + strand, within RPSI07_RS22285 at 3164.078 kb on - strand, within RPSI07_RS22285 at 3164.078 kb on - strand, within RPSI07_RS22285 at 3164.078 kb on - strand, within RPSI07_RS22285 at 3164.408 kb on - strand at 3164.408 kb on - strand at 3164.505 kb on + strand, within RPSI07_RS22290 at 3164.505 kb on + strand, within RPSI07_RS22290 at 3164.505 kb on + strand, within RPSI07_RS22290 at 3164.505 kb on + strand, within RPSI07_RS22290 at 3164.506 kb on - strand, within RPSI07_RS22290 at 3164.506 kb on - strand, within RPSI07_RS22290 at 3164.661 kb on + strand, within RPSI07_RS22290 at 3164.662 kb on - strand, within RPSI07_RS22290 at 3164.730 kb on + strand, within RPSI07_RS22290 at 3164.730 kb on + strand, within RPSI07_RS22290 at 3164.730 kb on + strand, within RPSI07_RS22290 at 3164.730 kb on + strand, within RPSI07_RS22290 at 3164.730 kb on + strand, within RPSI07_RS22290 at 3164.730 kb on + strand, within RPSI07_RS22290 at 3164.730 kb on + strand, within RPSI07_RS22290 at 3164.730 kb on + strand, within RPSI07_RS22290 at 3164.730 kb on + strand, within RPSI07_RS22290 at 3164.730 kb on + strand, within RPSI07_RS22290 at 3164.730 kb on + strand, within RPSI07_RS22290 at 3164.730 kb on + strand, within RPSI07_RS22290 at 3164.731 kb on - strand, within RPSI07_RS22290 at 3164.731 kb on - strand, within RPSI07_RS22290 at 3164.731 kb on - strand, within RPSI07_RS22290 at 3164.731 kb on - strand, within RPSI07_RS22290 at 3164.794 kb on + strand, within RPSI07_RS22290 at 3164.795 kb on - strand, within RPSI07_RS22290 at 3164.795 kb on - strand, within RPSI07_RS22290 at 3164.831 kb on - strand, within RPSI07_RS22290 at 3165.058 kb on - strand, within RPSI07_RS22290 at 3165.058 kb on - strand, within RPSI07_RS22290 at 3165.058 kb on - strand, within RPSI07_RS22290
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 3,162,795 + RPSI07_RS22280 0.37 +0.7 3,162,795 + RPSI07_RS22280 0.37 -0.5 3,162,796 - RPSI07_RS22280 0.37 -0.7 3,163,041 - RPSI07_RS22280 0.67 +1.9 3,163,227 + +0.5 3,163,228 - -1.4 3,163,302 + -0.2 3,163,327 - -0.7 3,163,333 + -0.9 3,163,333 + +2.4 3,163,333 + +1.1 3,163,333 + +2.5 3,163,333 + -0.4 3,163,333 + +1.1 3,163,333 + -0.1 3,163,334 - +1.4 3,163,334 - -0.1 3,163,334 - -0.8 3,163,334 - +0.2 3,163,334 - -1.3 3,163,334 - +0.6 3,163,334 - -0.5 3,163,334 - +2.1 3,163,334 - -0.9 3,163,690 + RPSI07_RS22285 0.32 +0.8 3,163,690 + RPSI07_RS22285 0.32 -0.1 3,163,690 + RPSI07_RS22285 0.32 -0.9 3,163,690 + RPSI07_RS22285 0.32 +0.2 3,163,691 - RPSI07_RS22285 0.32 -0.9 3,164,041 + RPSI07_RS22285 0.67 +1.1 3,164,041 + RPSI07_RS22285 0.67 -1.5 3,164,042 - RPSI07_RS22285 0.67 +0.7 3,164,042 - RPSI07_RS22285 0.67 +0.7 3,164,042 - RPSI07_RS22285 0.67 +0.9 3,164,077 + RPSI07_RS22285 0.70 +0.1 3,164,078 - RPSI07_RS22285 0.71 +0.1 3,164,078 - RPSI07_RS22285 0.71 -1.7 3,164,078 - RPSI07_RS22285 0.71 +0.5 3,164,408 - -1.3 3,164,408 - -0.3 3,164,505 + RPSI07_RS22290 0.14 +1.3 3,164,505 + RPSI07_RS22290 0.14 +1.1 3,164,505 + RPSI07_RS22290 0.14 -0.0 3,164,505 + RPSI07_RS22290 0.14 -1.1 3,164,506 - RPSI07_RS22290 0.14 +0.0 3,164,506 - RPSI07_RS22290 0.14 -0.6 3,164,661 + RPSI07_RS22290 0.31 +0.4 3,164,662 - RPSI07_RS22290 0.32 +0.5 3,164,730 + RPSI07_RS22290 0.39 +0.1 3,164,730 + RPSI07_RS22290 0.39 -1.3 3,164,730 + RPSI07_RS22290 0.39 -0.9 3,164,730 + RPSI07_RS22290 0.39 +0.1 3,164,730 + RPSI07_RS22290 0.39 -1.5 3,164,730 + RPSI07_RS22290 0.39 -0.5 3,164,730 + RPSI07_RS22290 0.39 +1.1 3,164,730 + RPSI07_RS22290 0.39 -1.5 3,164,730 + RPSI07_RS22290 0.39 -2.0 3,164,730 + RPSI07_RS22290 0.39 +0.6 3,164,730 + RPSI07_RS22290 0.39 -0.4 3,164,730 + RPSI07_RS22290 0.39 -2.6 3,164,731 - RPSI07_RS22290 0.40 -1.0 3,164,731 - RPSI07_RS22290 0.40 +1.9 3,164,731 - RPSI07_RS22290 0.40 +1.7 3,164,731 - RPSI07_RS22290 0.40 +1.3 3,164,794 + RPSI07_RS22290 0.47 -1.2 3,164,795 - RPSI07_RS22290 0.47 -0.3 3,164,795 - RPSI07_RS22290 0.47 +1.4 3,164,831 - RPSI07_RS22290 0.51 -0.3 3,165,058 - RPSI07_RS22290 0.77 +2.1 3,165,058 - RPSI07_RS22290 0.77 +1.9 3,165,058 - RPSI07_RS22290 0.77 +0.7
Or see this region's nucleotide sequence