Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS19555 and RPSI07_RS19560 are separated by 23 nucleotides RPSI07_RS19560 and RPSI07_RS19565 are separated by 59 nucleotides RPSI07_RS19565 and RPSI07_RS19570 are separated by 145 nucleotides
RPSI07_RS19555: RPSI07_RS19555 - tRNA (guanosine(37)-N1)-methyltransferase TrmD, at 2,568,338 to 2,569,126
_RS19555
RPSI07_RS19560: RPSI07_RS19560 - ribosome maturation factor RimM, at 2,569,150 to 2,569,791
_RS19560
RPSI07_RS19565: RPSI07_RS19565 - 30S ribosomal protein S16, at 2,569,851 to 2,570,105
_RS19565
RPSI07_RS19570: RPSI07_RS19570 - sorbosone dehydrogenase, at 2,570,251 to 2,571,324
_RS19570
Position (kb)
2569
2570
2571 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2569.810 kb on - strand at 2569.810 kb on - strand at 2569.810 kb on - strand at 2570.234 kb on - strand at 2570.402 kb on - strand, within RPSI07_RS19570 at 2570.402 kb on - strand, within RPSI07_RS19570 at 2570.428 kb on + strand, within RPSI07_RS19570 at 2570.428 kb on + strand, within RPSI07_RS19570 at 2570.524 kb on + strand, within RPSI07_RS19570 at 2570.524 kb on + strand, within RPSI07_RS19570 at 2570.524 kb on + strand, within RPSI07_RS19570 at 2570.524 kb on + strand, within RPSI07_RS19570 at 2570.524 kb on + strand, within RPSI07_RS19570 at 2570.525 kb on - strand, within RPSI07_RS19570 at 2570.525 kb on - strand, within RPSI07_RS19570 at 2570.674 kb on + strand, within RPSI07_RS19570 at 2570.675 kb on - strand, within RPSI07_RS19570 at 2570.675 kb on - strand, within RPSI07_RS19570 at 2570.989 kb on + strand, within RPSI07_RS19570 at 2570.989 kb on + strand, within RPSI07_RS19570 at 2570.989 kb on + strand, within RPSI07_RS19570 at 2570.989 kb on + strand, within RPSI07_RS19570 at 2570.990 kb on - strand, within RPSI07_RS19570 at 2570.990 kb on - strand, within RPSI07_RS19570 at 2570.990 kb on - strand, within RPSI07_RS19570 at 2571.046 kb on + strand, within RPSI07_RS19570 at 2571.046 kb on + strand, within RPSI07_RS19570 at 2571.046 kb on + strand, within RPSI07_RS19570 at 2571.047 kb on - strand, within RPSI07_RS19570 at 2571.074 kb on - strand, within RPSI07_RS19570
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 2,569,810 - +1.6 2,569,810 - -0.0 2,569,810 - +1.0 2,570,234 - +0.0 2,570,402 - RPSI07_RS19570 0.14 -0.9 2,570,402 - RPSI07_RS19570 0.14 -1.8 2,570,428 + RPSI07_RS19570 0.16 -0.7 2,570,428 + RPSI07_RS19570 0.16 -0.7 2,570,524 + RPSI07_RS19570 0.25 +0.3 2,570,524 + RPSI07_RS19570 0.25 -1.6 2,570,524 + RPSI07_RS19570 0.25 -0.2 2,570,524 + RPSI07_RS19570 0.25 +1.8 2,570,524 + RPSI07_RS19570 0.25 -1.7 2,570,525 - RPSI07_RS19570 0.26 -0.7 2,570,525 - RPSI07_RS19570 0.26 -0.3 2,570,674 + RPSI07_RS19570 0.39 -0.0 2,570,675 - RPSI07_RS19570 0.39 -2.6 2,570,675 - RPSI07_RS19570 0.39 +4.0 2,570,989 + RPSI07_RS19570 0.69 -2.2 2,570,989 + RPSI07_RS19570 0.69 -0.2 2,570,989 + RPSI07_RS19570 0.69 -0.0 2,570,989 + RPSI07_RS19570 0.69 -0.2 2,570,990 - RPSI07_RS19570 0.69 +4.2 2,570,990 - RPSI07_RS19570 0.69 +2.2 2,570,990 - RPSI07_RS19570 0.69 -2.2 2,571,046 + RPSI07_RS19570 0.74 +1.0 2,571,046 + RPSI07_RS19570 0.74 -0.2 2,571,046 + RPSI07_RS19570 0.74 -1.9 2,571,047 - RPSI07_RS19570 0.74 +1.8 2,571,074 - RPSI07_RS19570 0.77 +0.3
Or see this region's nucleotide sequence