Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS23565

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS23555 and RPSI07_RS23560 are separated by 64 nucleotidesRPSI07_RS23560 and RPSI07_RS23565 are separated by 36 nucleotidesRPSI07_RS23565 and RPSI07_RS23570 overlap by 4 nucleotidesRPSI07_RS23570 and RPSI07_RS23575 are separated by 13 nucleotides RPSI07_RS23555: RPSI07_RS23555 - tRNA-Phe, at 3,426,852 to 3,426,927 _RS23555 RPSI07_RS23560: RPSI07_RS23560 - two-component system response regulator, at 3,426,992 to 3,427,711 _RS23560 RPSI07_RS23565: RPSI07_RS23565 - PAS domain-containing sensor histidine kinase, at 3,427,748 to 3,430,183 _RS23565 RPSI07_RS23570: RPSI07_RS23570 - DUF4390 domain-containing protein, at 3,430,180 to 3,430,980 _RS23570 RPSI07_RS23575: RPSI07_RS23575 - 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB, at 3,430,994 to 3,432,319 _RS23575 Position (kb) 3427 3428 3429 3430 3431Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3427.723 kb on - strandat 3427.723 kb on - strandat 3427.723 kb on - strandat 3427.723 kb on - strandat 3427.723 kb on - strandat 3427.750 kb on - strandat 3427.750 kb on - strandat 3427.761 kb on - strandat 3428.009 kb on + strand, within RPSI07_RS23565at 3428.216 kb on + strand, within RPSI07_RS23565at 3428.280 kb on + strand, within RPSI07_RS23565at 3429.151 kb on - strand, within RPSI07_RS23565at 3429.151 kb on - strand, within RPSI07_RS23565at 3429.879 kb on + strand, within RPSI07_RS23565at 3429.880 kb on - strand, within RPSI07_RS23565at 3430.075 kb on + strandat 3430.164 kb on + strandat 3430.165 kb on - strandat 3430.165 kb on - strandat 3431.020 kb on - strandat 3431.023 kb on - strandat 3431.023 kb on - strandat 3431.124 kb on - strandat 3431.124 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
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3,427,723 - -0.1
3,427,723 - -3.7
3,427,723 - -1.8
3,427,723 - -0.2
3,427,723 - -1.4
3,427,750 - +2.3
3,427,750 - -0.8
3,427,761 - -0.6
3,428,009 + RPSI07_RS23565 0.11 -1.8
3,428,216 + RPSI07_RS23565 0.19 -1.3
3,428,280 + RPSI07_RS23565 0.22 -1.3
3,429,151 - RPSI07_RS23565 0.58 -2.6
3,429,151 - RPSI07_RS23565 0.58 -1.7
3,429,879 + RPSI07_RS23565 0.87 +0.7
3,429,880 - RPSI07_RS23565 0.88 +1.4
3,430,075 + -0.9
3,430,164 + +0.6
3,430,165 - +0.3
3,430,165 - -3.1
3,431,020 - +0.4
3,431,023 - +0.2
3,431,023 - +0.2
3,431,124 - -0.1
3,431,124 - +0.7

Or see this region's nucleotide sequence