Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS21465

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS21455 and RPSI07_RS21460 overlap by 14 nucleotidesRPSI07_RS21460 and RPSI07_RS21465 are separated by 30 nucleotidesRPSI07_RS21465 and RPSI07_RS21470 are separated by 99 nucleotides RPSI07_RS21455: RPSI07_RS21455 - EamA/RhaT family transporter, at 2,975,145 to 2,976,050 _RS21455 RPSI07_RS21460: RPSI07_RS21460 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA, at 2,976,037 to 2,976,882 _RS21460 RPSI07_RS21465: RPSI07_RS21465 - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 2,976,913 to 2,977,929 _RS21465 RPSI07_RS21470: RPSI07_RS21470 - molecular chaperone SurA, at 2,978,029 to 2,979,519 _RS21470 Position (kb) 2976 2977 2978Strain fitness (log2 ratio) -2 -1 0 1at 2976.377 kb on + strandat 2976.378 kb on - strand, within RPSI07_RS21460at 2976.378 kb on - strand, within RPSI07_RS21460at 2976.381 kb on - strand, within RPSI07_RS21460at 2976.537 kb on - strand, within RPSI07_RS21460at 2978.030 kb on + strandat 2978.031 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
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2,976,377 + +0.7
2,976,378 - RPSI07_RS21460 0.40 +1.7
2,976,378 - RPSI07_RS21460 0.40 +0.1
2,976,381 - RPSI07_RS21460 0.41 -2.6
2,976,537 - RPSI07_RS21460 0.59 +0.7
2,978,030 + +0.7
2,978,031 - +0.9

Or see this region's nucleotide sequence