Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21035 and RPSI07_RS21040 are separated by 434 nucleotides RPSI07_RS21040 and RPSI07_RS21045 are separated by 312 nucleotides RPSI07_RS21045 and RPSI07_RS21050 are separated by 55 nucleotides
RPSI07_RS21035: RPSI07_RS21035 - hypothetical protein, at 2,884,291 to 2,885,277
_RS21035
RPSI07_RS21040: RPSI07_RS21040 - HD-GYP domain-containing protein, at 2,885,712 to 2,886,923
_RS21040
RPSI07_RS21045: RPSI07_RS21045 - hypothetical protein, at 2,887,236 to 2,887,586
_RS21045
RPSI07_RS21050: RPSI07_RS21050 - MarR family transcriptional regulator, at 2,887,642 to 2,888,154
_RS21050
Position (kb)
2885
2886
2887 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 2884.790 kb on + strand, within RPSI07_RS21035 at 2884.790 kb on + strand, within RPSI07_RS21035 at 2884.790 kb on + strand, within RPSI07_RS21035 at 2884.791 kb on - strand, within RPSI07_RS21035 at 2884.791 kb on - strand, within RPSI07_RS21035 at 2884.817 kb on + strand, within RPSI07_RS21035 at 2884.818 kb on - strand, within RPSI07_RS21035 at 2884.818 kb on - strand, within RPSI07_RS21035 at 2885.387 kb on + strand at 2885.387 kb on + strand at 2885.439 kb on - strand at 2885.475 kb on + strand at 2885.476 kb on - strand at 2885.527 kb on + strand at 2885.527 kb on + strand at 2885.527 kb on + strand at 2885.527 kb on + strand at 2885.528 kb on - strand at 2885.848 kb on + strand, within RPSI07_RS21040 at 2885.848 kb on + strand, within RPSI07_RS21040 at 2885.849 kb on - strand, within RPSI07_RS21040 at 2885.849 kb on - strand, within RPSI07_RS21040 at 2885.849 kb on - strand, within RPSI07_RS21040 at 2885.849 kb on - strand, within RPSI07_RS21040 at 2885.966 kb on + strand, within RPSI07_RS21040 at 2886.019 kb on + strand, within RPSI07_RS21040 at 2886.019 kb on + strand, within RPSI07_RS21040 at 2886.019 kb on + strand, within RPSI07_RS21040 at 2886.019 kb on + strand, within RPSI07_RS21040 at 2886.019 kb on + strand, within RPSI07_RS21040 at 2886.019 kb on + strand, within RPSI07_RS21040 at 2886.020 kb on - strand, within RPSI07_RS21040 at 2886.020 kb on - strand, within RPSI07_RS21040 at 2886.055 kb on + strand, within RPSI07_RS21040 at 2886.055 kb on + strand, within RPSI07_RS21040 at 2886.056 kb on - strand, within RPSI07_RS21040 at 2886.056 kb on - strand, within RPSI07_RS21040 at 2886.082 kb on + strand, within RPSI07_RS21040 at 2886.082 kb on + strand, within RPSI07_RS21040 at 2886.082 kb on + strand, within RPSI07_RS21040 at 2886.083 kb on - strand, within RPSI07_RS21040 at 2886.439 kb on + strand, within RPSI07_RS21040 at 2886.439 kb on + strand, within RPSI07_RS21040 at 2886.439 kb on + strand, within RPSI07_RS21040 at 2886.439 kb on + strand, within RPSI07_RS21040 at 2886.440 kb on - strand, within RPSI07_RS21040 at 2886.440 kb on - strand, within RPSI07_RS21040 at 2886.440 kb on - strand, within RPSI07_RS21040 at 2886.440 kb on - strand, within RPSI07_RS21040 at 2886.445 kb on + strand, within RPSI07_RS21040 at 2886.446 kb on - strand, within RPSI07_RS21040 at 2886.446 kb on - strand, within RPSI07_RS21040 at 2886.487 kb on + strand, within RPSI07_RS21040 at 2886.488 kb on - strand, within RPSI07_RS21040 at 2886.488 kb on - strand, within RPSI07_RS21040 at 2886.488 kb on - strand, within RPSI07_RS21040 at 2886.488 kb on - strand, within RPSI07_RS21040 at 2886.657 kb on - strand, within RPSI07_RS21040 at 2886.923 kb on + strand at 2886.924 kb on - strand at 2886.924 kb on - strand at 2886.928 kb on + strand at 2886.929 kb on - strand at 2886.929 kb on - strand at 2887.216 kb on + strand at 2887.217 kb on - strand at 2887.244 kb on + strand at 2887.245 kb on - strand at 2887.245 kb on - strand at 2887.245 kb on - strand at 2887.268 kb on - strand at 2887.268 kb on - strand at 2887.432 kb on - strand, within RPSI07_RS21045 at 2887.432 kb on - strand, within RPSI07_RS21045 at 2887.519 kb on - strand, within RPSI07_RS21045 at 2887.519 kb on - strand, within RPSI07_RS21045 at 2887.633 kb on + strand at 2887.633 kb on + strand at 2887.634 kb on - strand at 2887.636 kb on + strand at 2887.636 kb on + strand at 2887.636 kb on + strand at 2887.637 kb on - strand at 2887.637 kb on - strand at 2887.637 kb on - strand at 2887.637 kb on - strand at 2887.637 kb on - strand at 2887.860 kb on + strand, within RPSI07_RS21050 at 2887.860 kb on + strand, within RPSI07_RS21050
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 2,884,790 + RPSI07_RS21035 0.51 -1.7 2,884,790 + RPSI07_RS21035 0.51 -0.1 2,884,790 + RPSI07_RS21035 0.51 -0.9 2,884,791 - RPSI07_RS21035 0.51 +0.5 2,884,791 - RPSI07_RS21035 0.51 +1.0 2,884,817 + RPSI07_RS21035 0.53 +0.2 2,884,818 - RPSI07_RS21035 0.53 -0.6 2,884,818 - RPSI07_RS21035 0.53 -1.8 2,885,387 + -0.6 2,885,387 + +1.5 2,885,439 - +0.3 2,885,475 + +0.7 2,885,476 - +0.7 2,885,527 + -0.7 2,885,527 + -0.3 2,885,527 + +1.3 2,885,527 + +0.7 2,885,528 - -1.1 2,885,848 + RPSI07_RS21040 0.11 +0.2 2,885,848 + RPSI07_RS21040 0.11 -1.1 2,885,849 - RPSI07_RS21040 0.11 -0.6 2,885,849 - RPSI07_RS21040 0.11 -2.7 2,885,849 - RPSI07_RS21040 0.11 +2.7 2,885,849 - RPSI07_RS21040 0.11 -0.6 2,885,966 + RPSI07_RS21040 0.21 -0.1 2,886,019 + RPSI07_RS21040 0.25 +1.0 2,886,019 + RPSI07_RS21040 0.25 -0.3 2,886,019 + RPSI07_RS21040 0.25 -0.8 2,886,019 + RPSI07_RS21040 0.25 +0.9 2,886,019 + RPSI07_RS21040 0.25 -0.6 2,886,019 + RPSI07_RS21040 0.25 +3.1 2,886,020 - RPSI07_RS21040 0.25 -0.8 2,886,020 - RPSI07_RS21040 0.25 +0.7 2,886,055 + RPSI07_RS21040 0.28 +1.6 2,886,055 + RPSI07_RS21040 0.28 -1.0 2,886,056 - RPSI07_RS21040 0.28 +3.7 2,886,056 - RPSI07_RS21040 0.28 -0.4 2,886,082 + RPSI07_RS21040 0.31 -0.3 2,886,082 + RPSI07_RS21040 0.31 +1.8 2,886,082 + RPSI07_RS21040 0.31 -0.4 2,886,083 - RPSI07_RS21040 0.31 -0.9 2,886,439 + RPSI07_RS21040 0.60 -0.6 2,886,439 + RPSI07_RS21040 0.60 -0.4 2,886,439 + RPSI07_RS21040 0.60 -3.1 2,886,439 + RPSI07_RS21040 0.60 -0.0 2,886,440 - RPSI07_RS21040 0.60 -1.3 2,886,440 - RPSI07_RS21040 0.60 +0.7 2,886,440 - RPSI07_RS21040 0.60 -0.3 2,886,440 - RPSI07_RS21040 0.60 -0.9 2,886,445 + RPSI07_RS21040 0.60 +3.2 2,886,446 - RPSI07_RS21040 0.61 +0.5 2,886,446 - RPSI07_RS21040 0.61 -1.1 2,886,487 + RPSI07_RS21040 0.64 -2.5 2,886,488 - RPSI07_RS21040 0.64 +1.7 2,886,488 - RPSI07_RS21040 0.64 +7.9 2,886,488 - RPSI07_RS21040 0.64 -0.4 2,886,488 - RPSI07_RS21040 0.64 +0.3 2,886,657 - RPSI07_RS21040 0.78 +2.3 2,886,923 + -1.4 2,886,924 - -1.2 2,886,924 - +0.1 2,886,928 + +0.4 2,886,929 - -0.6 2,886,929 - +2.5 2,887,216 + +0.2 2,887,217 - +0.7 2,887,244 + -0.3 2,887,245 - +0.1 2,887,245 - -0.8 2,887,245 - -2.6 2,887,268 - -1.6 2,887,268 - -0.0 2,887,432 - RPSI07_RS21045 0.56 +0.4 2,887,432 - RPSI07_RS21045 0.56 -0.3 2,887,519 - RPSI07_RS21045 0.81 -1.0 2,887,519 - RPSI07_RS21045 0.81 -0.5 2,887,633 + +0.1 2,887,633 + -0.1 2,887,634 - -0.6 2,887,636 + +0.1 2,887,636 + +2.1 2,887,636 + -3.2 2,887,637 - -1.5 2,887,637 - -1.0 2,887,637 - +1.0 2,887,637 - -0.2 2,887,637 - -0.3 2,887,860 + RPSI07_RS21050 0.42 +1.7 2,887,860 + RPSI07_RS21050 0.42 +0.4
Or see this region's nucleotide sequence