Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS18745 and RPSI07_RS18750 are separated by 64 nucleotides RPSI07_RS18750 and RPSI07_RS18755 are separated by 21 nucleotides RPSI07_RS18755 and RPSI07_RS18760 are separated by 623 nucleotides RPSI07_RS18760 and RPSI07_RS18765 are separated by 83 nucleotides
RPSI07_RS18745: RPSI07_RS18745 - pentapeptide MXKDX repeat protein, at 2,399,338 to 2,399,601
_RS18745
RPSI07_RS18750: RPSI07_RS18750 - hypothetical protein, at 2,399,666 to 2,400,439
_RS18750
RPSI07_RS18755: RPSI07_RS18755 - cytochrome b/b6 domain-containing protein, at 2,400,461 to 2,401,099
_RS18755
RPSI07_RS18760: RPSI07_RS18760 - thiamine biosynthesis protein ThiS, at 2,401,723 to 2,401,992
_RS18760
RPSI07_RS18765: RPSI07_RS18765 - hypothetical protein, at 2,402,076 to 2,403,236
_RS18765
Position (kb)
2400
2401
2402 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2399.621 kb on + strand at 2399.621 kb on + strand at 2399.622 kb on - strand at 2399.622 kb on - strand at 2399.653 kb on + strand at 2399.654 kb on - strand at 2399.689 kb on - strand at 2399.707 kb on - strand at 2399.719 kb on - strand at 2399.725 kb on + strand at 2399.725 kb on + strand at 2399.726 kb on - strand at 2399.776 kb on + strand, within RPSI07_RS18750 at 2399.777 kb on - strand, within RPSI07_RS18750 at 2399.777 kb on - strand, within RPSI07_RS18750 at 2399.832 kb on + strand, within RPSI07_RS18750 at 2399.832 kb on + strand, within RPSI07_RS18750 at 2399.832 kb on + strand, within RPSI07_RS18750 at 2399.832 kb on + strand, within RPSI07_RS18750 at 2399.832 kb on + strand, within RPSI07_RS18750 at 2399.832 kb on + strand, within RPSI07_RS18750 at 2399.833 kb on - strand, within RPSI07_RS18750 at 2399.833 kb on - strand, within RPSI07_RS18750 at 2399.833 kb on - strand, within RPSI07_RS18750 at 2399.889 kb on + strand, within RPSI07_RS18750 at 2399.889 kb on + strand, within RPSI07_RS18750 at 2399.889 kb on + strand, within RPSI07_RS18750 at 2399.890 kb on - strand, within RPSI07_RS18750 at 2399.890 kb on - strand, within RPSI07_RS18750 at 2399.890 kb on - strand, within RPSI07_RS18750 at 2399.890 kb on - strand, within RPSI07_RS18750 at 2399.890 kb on - strand, within RPSI07_RS18750 at 2399.893 kb on - strand, within RPSI07_RS18750 at 2399.919 kb on + strand, within RPSI07_RS18750 at 2399.919 kb on + strand, within RPSI07_RS18750 at 2399.919 kb on + strand, within RPSI07_RS18750 at 2399.919 kb on + strand, within RPSI07_RS18750 at 2399.919 kb on + strand, within RPSI07_RS18750 at 2399.919 kb on + strand, within RPSI07_RS18750 at 2399.920 kb on - strand, within RPSI07_RS18750 at 2399.920 kb on - strand, within RPSI07_RS18750 at 2399.920 kb on - strand, within RPSI07_RS18750 at 2399.920 kb on - strand, within RPSI07_RS18750 at 2399.920 kb on - strand, within RPSI07_RS18750 at 2399.920 kb on - strand, within RPSI07_RS18750 at 2399.920 kb on - strand, within RPSI07_RS18750 at 2399.920 kb on - strand, within RPSI07_RS18750 at 2399.920 kb on - strand, within RPSI07_RS18750 at 2399.933 kb on - strand, within RPSI07_RS18750 at 2400.014 kb on - strand, within RPSI07_RS18750 at 2400.014 kb on - strand, within RPSI07_RS18750 at 2400.014 kb on - strand, within RPSI07_RS18750 at 2400.048 kb on + strand, within RPSI07_RS18750 at 2400.048 kb on + strand, within RPSI07_RS18750 at 2400.048 kb on + strand at 2400.048 kb on + strand, within RPSI07_RS18750 at 2400.049 kb on - strand, within RPSI07_RS18750 at 2400.150 kb on + strand, within RPSI07_RS18750 at 2400.150 kb on + strand, within RPSI07_RS18750 at 2400.150 kb on + strand, within RPSI07_RS18750 at 2400.151 kb on - strand, within RPSI07_RS18750 at 2400.151 kb on - strand, within RPSI07_RS18750 at 2400.151 kb on - strand, within RPSI07_RS18750 at 2400.192 kb on + strand, within RPSI07_RS18750 at 2400.392 kb on + strand at 2400.392 kb on + strand at 2400.410 kb on + strand at 2400.410 kb on + strand at 2400.582 kb on + strand, within RPSI07_RS18755 at 2400.653 kb on + strand, within RPSI07_RS18755 at 2400.654 kb on - strand, within RPSI07_RS18755 at 2400.681 kb on + strand, within RPSI07_RS18755 at 2400.682 kb on - strand, within RPSI07_RS18755 at 2400.705 kb on + strand, within RPSI07_RS18755 at 2400.706 kb on - strand, within RPSI07_RS18755 at 2400.832 kb on - strand, within RPSI07_RS18755 at 2400.832 kb on - strand, within RPSI07_RS18755 at 2400.954 kb on + strand, within RPSI07_RS18755 at 2400.954 kb on + strand, within RPSI07_RS18755 at 2400.954 kb on + strand, within RPSI07_RS18755 at 2400.955 kb on - strand, within RPSI07_RS18755 at 2400.958 kb on + strand, within RPSI07_RS18755 at 2400.958 kb on + strand, within RPSI07_RS18755 at 2401.140 kb on + strand at 2401.140 kb on + strand at 2401.141 kb on - strand at 2401.141 kb on - strand at 2401.141 kb on - strand at 2401.294 kb on + strand at 2401.294 kb on + strand at 2401.295 kb on - strand at 2401.295 kb on - strand at 2401.296 kb on + strand at 2401.297 kb on - strand at 2401.302 kb on + strand at 2401.303 kb on - strand at 2401.303 kb on - strand at 2401.331 kb on + strand at 2401.331 kb on + strand at 2401.331 kb on + strand at 2401.331 kb on + strand at 2401.331 kb on + strand at 2401.332 kb on - strand at 2401.332 kb on - strand at 2401.332 kb on - strand at 2401.350 kb on + strand at 2401.351 kb on - strand at 2401.351 kb on - strand at 2401.629 kb on + strand at 2401.641 kb on + strand at 2401.642 kb on - strand at 2401.675 kb on + strand at 2401.675 kb on + strand at 2401.676 kb on - strand at 2401.676 kb on - strand at 2401.676 kb on - strand at 2401.680 kb on + strand at 2401.680 kb on + strand at 2401.680 kb on + strand at 2401.680 kb on + strand at 2401.680 kb on + strand at 2401.681 kb on - strand at 2401.681 kb on - strand at 2401.681 kb on - strand at 2401.681 kb on - strand at 2401.849 kb on + strand, within RPSI07_RS18760 at 2401.964 kb on - strand, within RPSI07_RS18760 at 2401.964 kb on - strand, within RPSI07_RS18760 at 2401.964 kb on - strand, within RPSI07_RS18760
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 2,399,621 + -2.6 2,399,621 + -2.1 2,399,622 - -0.9 2,399,622 - -0.4 2,399,653 + -0.4 2,399,654 - +2.2 2,399,689 - +0.1 2,399,707 - -0.2 2,399,719 - -0.4 2,399,725 + -2.1 2,399,725 + +0.5 2,399,726 - +0.1 2,399,776 + RPSI07_RS18750 0.14 -0.3 2,399,777 - RPSI07_RS18750 0.14 +2.3 2,399,777 - RPSI07_RS18750 0.14 -0.0 2,399,832 + RPSI07_RS18750 0.21 -2.7 2,399,832 + RPSI07_RS18750 0.21 +0.3 2,399,832 + RPSI07_RS18750 0.21 -0.8 2,399,832 + RPSI07_RS18750 0.21 +3.5 2,399,832 + RPSI07_RS18750 0.21 +0.5 2,399,832 + RPSI07_RS18750 0.21 +0.1 2,399,833 - RPSI07_RS18750 0.22 +0.6 2,399,833 - RPSI07_RS18750 0.22 -0.4 2,399,833 - RPSI07_RS18750 0.22 +1.6 2,399,889 + RPSI07_RS18750 0.29 -1.1 2,399,889 + RPSI07_RS18750 0.29 -0.2 2,399,889 + RPSI07_RS18750 0.29 -1.2 2,399,890 - RPSI07_RS18750 0.29 -0.0 2,399,890 - RPSI07_RS18750 0.29 -0.5 2,399,890 - RPSI07_RS18750 0.29 +2.4 2,399,890 - RPSI07_RS18750 0.29 -0.8 2,399,890 - RPSI07_RS18750 0.29 -1.7 2,399,893 - RPSI07_RS18750 0.29 -0.5 2,399,919 + RPSI07_RS18750 0.33 +0.9 2,399,919 + RPSI07_RS18750 0.33 +0.3 2,399,919 + RPSI07_RS18750 0.33 -0.2 2,399,919 + RPSI07_RS18750 0.33 -0.7 2,399,919 + RPSI07_RS18750 0.33 -1.9 2,399,919 + RPSI07_RS18750 0.33 -0.4 2,399,920 - RPSI07_RS18750 0.33 -0.2 2,399,920 - RPSI07_RS18750 0.33 +0.0 2,399,920 - RPSI07_RS18750 0.33 +0.6 2,399,920 - RPSI07_RS18750 0.33 -1.7 2,399,920 - RPSI07_RS18750 0.33 +0.6 2,399,920 - RPSI07_RS18750 0.33 -1.0 2,399,920 - RPSI07_RS18750 0.33 +0.1 2,399,920 - RPSI07_RS18750 0.33 -0.7 2,399,920 - RPSI07_RS18750 0.33 -0.4 2,399,933 - RPSI07_RS18750 0.34 -0.4 2,400,014 - RPSI07_RS18750 0.45 -0.3 2,400,014 - RPSI07_RS18750 0.45 +0.1 2,400,014 - RPSI07_RS18750 0.45 +2.6 2,400,048 + RPSI07_RS18750 0.49 +0.6 2,400,048 + RPSI07_RS18750 0.49 +1.7 2,400,048 + -1.0 2,400,048 + RPSI07_RS18750 0.49 -2.1 2,400,049 - RPSI07_RS18750 0.49 -0.5 2,400,150 + RPSI07_RS18750 0.63 -2.0 2,400,150 + RPSI07_RS18750 0.63 +0.5 2,400,150 + RPSI07_RS18750 0.63 -0.6 2,400,151 - RPSI07_RS18750 0.63 +0.7 2,400,151 - RPSI07_RS18750 0.63 -0.1 2,400,151 - RPSI07_RS18750 0.63 -0.8 2,400,192 + RPSI07_RS18750 0.68 -1.3 2,400,392 + -1.8 2,400,392 + -0.7 2,400,410 + -1.0 2,400,410 + -0.2 2,400,582 + RPSI07_RS18755 0.19 +3.6 2,400,653 + RPSI07_RS18755 0.30 -0.9 2,400,654 - RPSI07_RS18755 0.30 -0.7 2,400,681 + RPSI07_RS18755 0.34 +0.3 2,400,682 - RPSI07_RS18755 0.35 -0.8 2,400,705 + RPSI07_RS18755 0.38 +0.1 2,400,706 - RPSI07_RS18755 0.38 -0.4 2,400,832 - RPSI07_RS18755 0.58 -1.2 2,400,832 - RPSI07_RS18755 0.58 -2.0 2,400,954 + RPSI07_RS18755 0.77 -1.6 2,400,954 + RPSI07_RS18755 0.77 +0.4 2,400,954 + RPSI07_RS18755 0.77 +0.1 2,400,955 - RPSI07_RS18755 0.77 -2.0 2,400,958 + RPSI07_RS18755 0.78 -0.3 2,400,958 + RPSI07_RS18755 0.78 +0.4 2,401,140 + +1.5 2,401,140 + +1.2 2,401,141 - +1.4 2,401,141 - +0.2 2,401,141 - -1.5 2,401,294 + -1.3 2,401,294 + +0.6 2,401,295 - +2.2 2,401,295 - -1.1 2,401,296 + +1.5 2,401,297 - -1.7 2,401,302 + -1.6 2,401,303 - -0.7 2,401,303 - +0.7 2,401,331 + +2.2 2,401,331 + -1.1 2,401,331 + -0.6 2,401,331 + -0.6 2,401,331 + -0.0 2,401,332 - -0.4 2,401,332 - -0.4 2,401,332 - -2.2 2,401,350 + +0.6 2,401,351 - -0.3 2,401,351 - +2.2 2,401,629 + -0.5 2,401,641 + +0.3 2,401,642 - +0.3 2,401,675 + -0.5 2,401,675 + -0.1 2,401,676 - -0.1 2,401,676 - -1.4 2,401,676 - +0.6 2,401,680 + -2.1 2,401,680 + -1.2 2,401,680 + -0.2 2,401,680 + -0.0 2,401,680 + -1.1 2,401,681 - +2.4 2,401,681 - -1.3 2,401,681 - -2.3 2,401,681 - -1.7 2,401,849 + RPSI07_RS18760 0.47 +0.4 2,401,964 - RPSI07_RS18760 0.89 +0.6 2,401,964 - RPSI07_RS18760 0.89 +1.0 2,401,964 - RPSI07_RS18760 0.89 +1.2
Or see this region's nucleotide sequence