Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS13920 and RPSI07_RS13925 are separated by 1 nucleotides RPSI07_RS13925 and RPSI07_RS13930 are separated by 3 nucleotides RPSI07_RS13930 and RPSI07_RS13935 are separated by 3 nucleotides RPSI07_RS13935 and RPSI07_RS13940 overlap by 14 nucleotides
RPSI07_RS13920: RPSI07_RS13920 - type II/IV secretion system protein, at 1,303,921 to 1,305,546
_RS13920
RPSI07_RS13925: RPSI07_RS13925 - type II secretion system F family protein, at 1,305,548 to 1,306,741
_RS13925
RPSI07_RS13930: RPSI07_RS13930 - type II secretion system protein GspG, at 1,306,745 to 1,307,179
_RS13930
RPSI07_RS13935: RPSI07_RS13935 - type II secretion system protein, at 1,307,183 to 1,307,647
_RS13935
RPSI07_RS13940: RPSI07_RS13940 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 1,307,634 to 1,308,020
_RS13940
Position (kb)
1305
1306
1307 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1305.016 kb on + strand, within RPSI07_RS13920 at 1305.016 kb on + strand, within RPSI07_RS13920 at 1305.020 kb on - strand, within RPSI07_RS13920 at 1305.037 kb on + strand, within RPSI07_RS13920 at 1305.038 kb on - strand, within RPSI07_RS13920 at 1305.038 kb on - strand, within RPSI07_RS13920 at 1305.038 kb on - strand, within RPSI07_RS13920 at 1305.546 kb on + strand at 1305.547 kb on - strand at 1305.547 kb on - strand at 1305.547 kb on - strand at 1305.574 kb on + strand at 1305.582 kb on + strand at 1305.582 kb on + strand at 1305.582 kb on + strand at 1305.582 kb on + strand at 1305.582 kb on + strand at 1305.582 kb on + strand at 1305.582 kb on + strand at 1305.582 kb on + strand at 1305.583 kb on - strand at 1305.583 kb on - strand at 1305.583 kb on - strand at 1305.583 kb on - strand at 1305.583 kb on - strand at 1305.687 kb on + strand, within RPSI07_RS13925 at 1305.687 kb on + strand, within RPSI07_RS13925 at 1305.688 kb on - strand, within RPSI07_RS13925 at 1305.906 kb on + strand, within RPSI07_RS13925 at 1305.986 kb on + strand, within RPSI07_RS13925 at 1305.987 kb on - strand, within RPSI07_RS13925 at 1306.164 kb on + strand, within RPSI07_RS13925 at 1306.165 kb on - strand, within RPSI07_RS13925 at 1306.191 kb on + strand, within RPSI07_RS13925 at 1306.191 kb on + strand, within RPSI07_RS13925 at 1306.191 kb on + strand, within RPSI07_RS13925 at 1306.191 kb on + strand, within RPSI07_RS13925 at 1306.192 kb on - strand, within RPSI07_RS13925 at 1306.192 kb on - strand, within RPSI07_RS13925 at 1306.209 kb on - strand, within RPSI07_RS13925 at 1306.209 kb on - strand, within RPSI07_RS13925 at 1306.305 kb on + strand, within RPSI07_RS13925 at 1306.438 kb on - strand, within RPSI07_RS13925 at 1306.450 kb on - strand, within RPSI07_RS13925 at 1306.801 kb on - strand, within RPSI07_RS13930 at 1306.815 kb on + strand, within RPSI07_RS13930 at 1306.877 kb on + strand, within RPSI07_RS13930 at 1306.894 kb on - strand, within RPSI07_RS13930 at 1307.064 kb on + strand, within RPSI07_RS13930 at 1307.079 kb on + strand, within RPSI07_RS13930 at 1307.080 kb on - strand, within RPSI07_RS13930 at 1307.132 kb on - strand, within RPSI07_RS13930 at 1307.223 kb on + strand at 1307.224 kb on - strand at 1307.265 kb on + strand, within RPSI07_RS13935 at 1307.298 kb on + strand, within RPSI07_RS13935 at 1307.299 kb on - strand, within RPSI07_RS13935 at 1307.299 kb on - strand, within RPSI07_RS13935 at 1307.299 kb on - strand, within RPSI07_RS13935 at 1307.469 kb on + strand, within RPSI07_RS13935 at 1307.469 kb on + strand, within RPSI07_RS13935 at 1307.470 kb on - strand, within RPSI07_RS13935 at 1307.470 kb on - strand, within RPSI07_RS13935 at 1307.472 kb on + strand, within RPSI07_RS13935 at 1307.472 kb on + strand, within RPSI07_RS13935 at 1307.472 kb on + strand, within RPSI07_RS13935 at 1307.473 kb on + strand, within RPSI07_RS13935 at 1307.473 kb on - strand, within RPSI07_RS13935 at 1307.473 kb on - strand, within RPSI07_RS13935 at 1307.475 kb on + strand, within RPSI07_RS13935 at 1307.475 kb on + strand, within RPSI07_RS13935 at 1307.475 kb on + strand, within RPSI07_RS13935 at 1307.475 kb on + strand, within RPSI07_RS13935 at 1307.475 kb on + strand, within RPSI07_RS13935 at 1307.476 kb on - strand, within RPSI07_RS13935 at 1307.476 kb on - strand, within RPSI07_RS13935 at 1307.476 kb on - strand, within RPSI07_RS13935 at 1307.589 kb on - strand, within RPSI07_RS13935 at 1307.616 kb on + strand at 1307.647 kb on + strand at 1307.647 kb on + strand at 1307.647 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 1,305,016 + RPSI07_RS13920 0.67 +0.9 1,305,016 + RPSI07_RS13920 0.67 +1.2 1,305,020 - RPSI07_RS13920 0.68 -0.9 1,305,037 + RPSI07_RS13920 0.69 -0.1 1,305,038 - RPSI07_RS13920 0.69 -0.4 1,305,038 - RPSI07_RS13920 0.69 +1.1 1,305,038 - RPSI07_RS13920 0.69 -1.3 1,305,546 + -0.2 1,305,547 - +1.0 1,305,547 - +3.4 1,305,547 - -0.7 1,305,574 + +0.2 1,305,582 + +0.6 1,305,582 + -0.7 1,305,582 + +0.1 1,305,582 + -1.7 1,305,582 + +2.0 1,305,582 + -1.5 1,305,582 + +0.8 1,305,582 + -0.9 1,305,583 - -1.2 1,305,583 - -0.9 1,305,583 - -0.9 1,305,583 - +0.6 1,305,583 - +0.4 1,305,687 + RPSI07_RS13925 0.12 +1.4 1,305,687 + RPSI07_RS13925 0.12 +0.5 1,305,688 - RPSI07_RS13925 0.12 -1.7 1,305,906 + RPSI07_RS13925 0.30 -0.1 1,305,986 + RPSI07_RS13925 0.37 +0.3 1,305,987 - RPSI07_RS13925 0.37 -1.2 1,306,164 + RPSI07_RS13925 0.52 -1.3 1,306,165 - RPSI07_RS13925 0.52 +0.6 1,306,191 + RPSI07_RS13925 0.54 +1.2 1,306,191 + RPSI07_RS13925 0.54 +0.9 1,306,191 + RPSI07_RS13925 0.54 +2.1 1,306,191 + RPSI07_RS13925 0.54 -0.8 1,306,192 - RPSI07_RS13925 0.54 -0.6 1,306,192 - RPSI07_RS13925 0.54 -2.2 1,306,209 - RPSI07_RS13925 0.55 -0.4 1,306,209 - RPSI07_RS13925 0.55 +0.1 1,306,305 + RPSI07_RS13925 0.63 -0.0 1,306,438 - RPSI07_RS13925 0.75 -0.0 1,306,450 - RPSI07_RS13925 0.76 -2.1 1,306,801 - RPSI07_RS13930 0.13 +0.1 1,306,815 + RPSI07_RS13930 0.16 -0.7 1,306,877 + RPSI07_RS13930 0.30 -0.7 1,306,894 - RPSI07_RS13930 0.34 -0.2 1,307,064 + RPSI07_RS13930 0.73 -1.7 1,307,079 + RPSI07_RS13930 0.77 -0.1 1,307,080 - RPSI07_RS13930 0.77 -0.1 1,307,132 - RPSI07_RS13930 0.89 -0.7 1,307,223 + +0.3 1,307,224 - +0.0 1,307,265 + RPSI07_RS13935 0.18 +0.9 1,307,298 + RPSI07_RS13935 0.25 +1.6 1,307,299 - RPSI07_RS13935 0.25 -2.5 1,307,299 - RPSI07_RS13935 0.25 -1.6 1,307,299 - RPSI07_RS13935 0.25 -0.5 1,307,469 + RPSI07_RS13935 0.62 -0.5 1,307,469 + RPSI07_RS13935 0.62 +0.0 1,307,470 - RPSI07_RS13935 0.62 +0.1 1,307,470 - RPSI07_RS13935 0.62 -1.0 1,307,472 + RPSI07_RS13935 0.62 +0.6 1,307,472 + RPSI07_RS13935 0.62 -0.6 1,307,472 + RPSI07_RS13935 0.62 +0.6 1,307,473 + RPSI07_RS13935 0.62 +0.4 1,307,473 - RPSI07_RS13935 0.62 +0.4 1,307,473 - RPSI07_RS13935 0.62 -1.2 1,307,475 + RPSI07_RS13935 0.63 +1.2 1,307,475 + RPSI07_RS13935 0.63 -2.7 1,307,475 + RPSI07_RS13935 0.63 +0.4 1,307,475 + RPSI07_RS13935 0.63 -0.3 1,307,475 + RPSI07_RS13935 0.63 -1.3 1,307,476 - RPSI07_RS13935 0.63 +2.6 1,307,476 - RPSI07_RS13935 0.63 -2.6 1,307,476 - RPSI07_RS13935 0.63 +0.2 1,307,589 - RPSI07_RS13935 0.87 +0.7 1,307,616 + +0.4 1,307,647 + +0.2 1,307,647 + -0.4 1,307,647 + -0.5
Or see this region's nucleotide sequence