Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS11790 and RPSI07_RS11795 overlap by 4 nucleotides RPSI07_RS11795 and RPSI07_RS11800 are separated by 52 nucleotides RPSI07_RS11800 and RPSI07_RS11805 are separated by 8 nucleotides
RPSI07_RS11790: RPSI07_RS11790 - LrgB family protein, at 847,622 to 848,344
_RS11790
RPSI07_RS11795: RPSI07_RS11795 - CidA/LrgA family protein, at 848,341 to 848,718
_RS11795
RPSI07_RS11800: RPSI07_RS11800 - glycolate oxidase iron-sulfur subunit, at 848,771 to 850,018
_RS11800
RPSI07_RS11805: RPSI07_RS11805 - glycolate oxidase subunit GlcE, at 850,027 to 851,145
_RS11805
Position (kb) 848
849
850
851 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 847.788 kb on + strand, within RPSI07_RS11790 at 847.789 kb on - strand at 848.076 kb on + strand, within RPSI07_RS11790 at 848.145 kb on + strand, within RPSI07_RS11790 at 848.145 kb on + strand, within RPSI07_RS11790 at 848.145 kb on + strand, within RPSI07_RS11790 at 848.146 kb on - strand, within RPSI07_RS11790 at 848.146 kb on - strand, within RPSI07_RS11790 at 848.154 kb on + strand, within RPSI07_RS11790 at 848.154 kb on + strand, within RPSI07_RS11790 at 848.154 kb on + strand at 848.155 kb on - strand at 848.156 kb on - strand, within RPSI07_RS11790 at 848.245 kb on - strand, within RPSI07_RS11790 at 848.245 kb on - strand, within RPSI07_RS11790 at 848.245 kb on - strand, within RPSI07_RS11790 at 848.245 kb on - strand, within RPSI07_RS11790 at 848.245 kb on - strand, within RPSI07_RS11790 at 848.245 kb on - strand, within RPSI07_RS11790 at 848.249 kb on - strand, within RPSI07_RS11790 at 848.265 kb on + strand, within RPSI07_RS11790 at 848.266 kb on - strand, within RPSI07_RS11790 at 848.313 kb on + strand at 848.314 kb on - strand at 848.328 kb on + strand at 848.329 kb on - strand at 848.329 kb on - strand at 848.611 kb on + strand, within RPSI07_RS11795 at 848.612 kb on - strand, within RPSI07_RS11795 at 848.660 kb on + strand, within RPSI07_RS11795 at 848.661 kb on - strand, within RPSI07_RS11795 at 848.872 kb on + strand at 848.873 kb on - strand at 848.952 kb on + strand, within RPSI07_RS11800 at 848.953 kb on - strand, within RPSI07_RS11800 at 849.566 kb on + strand, within RPSI07_RS11800 at 849.566 kb on + strand, within RPSI07_RS11800 at 849.747 kb on + strand, within RPSI07_RS11800 at 849.748 kb on - strand, within RPSI07_RS11800 at 849.861 kb on + strand, within RPSI07_RS11800 at 849.861 kb on + strand, within RPSI07_RS11800 at 849.861 kb on + strand, within RPSI07_RS11800 at 849.862 kb on - strand, within RPSI07_RS11800 at 849.862 kb on - strand, within RPSI07_RS11800 at 849.862 kb on - strand, within RPSI07_RS11800 at 849.862 kb on - strand, within RPSI07_RS11800 at 849.907 kb on - strand at 849.907 kb on - strand at 849.907 kb on - strand at 849.953 kb on + strand at 849.954 kb on - strand at 850.041 kb on - strand at 850.973 kb on + strand, within RPSI07_RS11805 at 850.973 kb on + strand, within RPSI07_RS11805 at 850.974 kb on - strand, within RPSI07_RS11805 at 851.001 kb on + strand, within RPSI07_RS11805
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 847,788 + RPSI07_RS11790 0.23 -0.9 847,789 - +1.7 848,076 + RPSI07_RS11790 0.63 +0.4 848,145 + RPSI07_RS11790 0.72 +1.1 848,145 + RPSI07_RS11790 0.72 -0.6 848,145 + RPSI07_RS11790 0.72 -0.3 848,146 - RPSI07_RS11790 0.72 +0.5 848,146 - RPSI07_RS11790 0.72 -0.3 848,154 + RPSI07_RS11790 0.74 +0.6 848,154 + RPSI07_RS11790 0.74 -0.2 848,154 + +0.7 848,155 - +1.7 848,156 - RPSI07_RS11790 0.74 +0.6 848,245 - RPSI07_RS11790 0.86 +0.7 848,245 - RPSI07_RS11790 0.86 +1.5 848,245 - RPSI07_RS11790 0.86 +0.7 848,245 - RPSI07_RS11790 0.86 -0.4 848,245 - RPSI07_RS11790 0.86 +0.5 848,245 - RPSI07_RS11790 0.86 +0.7 848,249 - RPSI07_RS11790 0.87 -0.1 848,265 + RPSI07_RS11790 0.89 +0.2 848,266 - RPSI07_RS11790 0.89 +1.2 848,313 + -0.9 848,314 - -0.2 848,328 + -4.2 848,329 - +2.0 848,329 - +1.2 848,611 + RPSI07_RS11795 0.71 -0.0 848,612 - RPSI07_RS11795 0.72 -0.7 848,660 + RPSI07_RS11795 0.84 -0.8 848,661 - RPSI07_RS11795 0.85 +0.7 848,872 + +1.3 848,873 - +2.3 848,952 + RPSI07_RS11800 0.15 -1.5 848,953 - RPSI07_RS11800 0.15 +0.5 849,566 + RPSI07_RS11800 0.64 -1.2 849,566 + RPSI07_RS11800 0.64 -0.3 849,747 + RPSI07_RS11800 0.78 -1.0 849,748 - RPSI07_RS11800 0.78 +0.1 849,861 + RPSI07_RS11800 0.87 +0.8 849,861 + RPSI07_RS11800 0.87 +0.7 849,861 + RPSI07_RS11800 0.87 -0.0 849,862 - RPSI07_RS11800 0.87 -0.4 849,862 - RPSI07_RS11800 0.87 +0.7 849,862 - RPSI07_RS11800 0.87 -0.1 849,862 - RPSI07_RS11800 0.87 -0.9 849,907 - +1.4 849,907 - -0.6 849,907 - -2.1 849,953 + +0.1 849,954 - +0.7 850,041 - -0.3 850,973 + RPSI07_RS11805 0.85 +1.3 850,973 + RPSI07_RS11805 0.85 -1.6 850,974 - RPSI07_RS11805 0.85 -0.5 851,001 + RPSI07_RS11805 0.87 +0.4
Or see this region's nucleotide sequence