Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS24325 and RPSI07_RS03535 are separated by 115 nucleotides RPSI07_RS03535 and RPSI07_RS03540 are separated by 92 nucleotides
RPSI07_RS24325: RPSI07_RS24325 - hypothetical protein, at 959,199 to 959,930
_RS24325
RPSI07_RS03535: RPSI07_RS03535 - methyl-accepting chemotaxis protein, at 960,046 to 961,602
_RS03535
RPSI07_RS03540: RPSI07_RS03540 - hypothetical protein, at 961,695 to 962,348
_RS03540
Position (kb) 960
961
962 Strain fitness (log2 ratio) -2
-1
0
1
2 at 959.097 kb on + strand at 959.097 kb on + strand at 959.097 kb on + strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.513 kb on + strand, within RPSI07_RS24325 at 959.513 kb on + strand, within RPSI07_RS24325 at 959.513 kb on + strand, within RPSI07_RS24325 at 959.672 kb on + strand, within RPSI07_RS24325 at 959.673 kb on - strand, within RPSI07_RS24325 at 959.673 kb on - strand, within RPSI07_RS24325 at 959.673 kb on - strand, within RPSI07_RS24325 at 959.673 kb on - strand, within RPSI07_RS24325 at 959.826 kb on - strand, within RPSI07_RS24325 at 959.941 kb on + strand at 959.942 kb on - strand at 959.954 kb on + strand at 959.955 kb on - strand at 959.965 kb on + strand at 959.966 kb on - strand at 959.966 kb on - strand at 961.103 kb on + strand, within RPSI07_RS03535 at 961.134 kb on - strand, within RPSI07_RS03535 at 961.134 kb on - strand, within RPSI07_RS03535 at 961.236 kb on - strand, within RPSI07_RS03535 at 961.675 kb on + strand at 961.675 kb on + strand at 961.676 kb on - strand at 961.676 kb on - strand at 961.676 kb on - strand at 961.676 kb on - strand at 961.676 kb on - strand at 961.892 kb on + strand, within RPSI07_RS03540 at 961.893 kb on - strand, within RPSI07_RS03540 at 961.893 kb on - strand, within RPSI07_RS03540 at 961.893 kb on - strand, within RPSI07_RS03540 at 962.083 kb on + strand, within RPSI07_RS03540 at 962.083 kb on + strand, within RPSI07_RS03540 at 962.083 kb on + strand, within RPSI07_RS03540 at 962.083 kb on + strand, within RPSI07_RS03540 at 962.084 kb on - strand, within RPSI07_RS03540 at 962.084 kb on - strand, within RPSI07_RS03540 at 962.084 kb on - strand, within RPSI07_RS03540 at 962.084 kb on - strand, within RPSI07_RS03540 at 962.084 kb on - strand, within RPSI07_RS03540 at 962.237 kb on - strand, within RPSI07_RS03540 at 962.275 kb on + strand, within RPSI07_RS03540 at 962.382 kb on - strand at 962.382 kb on - strand at 962.587 kb on + strand at 962.587 kb on + strand at 962.587 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 959,097 + -1.6 959,097 + +0.9 959,097 + +0.2 959,098 - -0.4 959,098 - +1.7 959,098 - +0.2 959,098 - +0.5 959,098 - -0.5 959,098 - +2.0 959,513 + RPSI07_RS24325 0.43 -1.2 959,513 + RPSI07_RS24325 0.43 +0.7 959,513 + RPSI07_RS24325 0.43 +0.1 959,672 + RPSI07_RS24325 0.65 +0.6 959,673 - RPSI07_RS24325 0.65 +1.0 959,673 - RPSI07_RS24325 0.65 -0.7 959,673 - RPSI07_RS24325 0.65 +0.3 959,673 - RPSI07_RS24325 0.65 +0.1 959,826 - RPSI07_RS24325 0.86 +1.1 959,941 + -0.0 959,942 - -0.2 959,954 + -0.7 959,955 - -1.1 959,965 + -1.7 959,966 - -2.0 959,966 - +0.4 961,103 + RPSI07_RS03535 0.68 +0.7 961,134 - RPSI07_RS03535 0.70 +2.0 961,134 - RPSI07_RS03535 0.70 -0.4 961,236 - RPSI07_RS03535 0.76 +1.4 961,675 + +0.2 961,675 + -1.3 961,676 - -0.1 961,676 - -1.2 961,676 - +0.4 961,676 - +0.2 961,676 - -0.2 961,892 + RPSI07_RS03540 0.30 -0.6 961,893 - RPSI07_RS03540 0.30 +0.4 961,893 - RPSI07_RS03540 0.30 -0.6 961,893 - RPSI07_RS03540 0.30 +0.4 962,083 + RPSI07_RS03540 0.59 -1.2 962,083 + RPSI07_RS03540 0.59 -0.6 962,083 + RPSI07_RS03540 0.59 -0.4 962,083 + RPSI07_RS03540 0.59 +0.1 962,084 - RPSI07_RS03540 0.59 -0.8 962,084 - RPSI07_RS03540 0.59 -0.8 962,084 - RPSI07_RS03540 0.59 +0.9 962,084 - RPSI07_RS03540 0.59 +0.4 962,084 - RPSI07_RS03540 0.59 -0.8 962,237 - RPSI07_RS03540 0.83 -1.6 962,275 + RPSI07_RS03540 0.89 -1.0 962,382 - -2.0 962,382 - -0.3 962,587 + +0.2 962,587 + -1.3 962,587 + -0.5
Or see this region's nucleotide sequence