Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS18790 and RPSI07_RS18795 are separated by 103 nucleotides RPSI07_RS18795 and RPSI07_RS18800 are separated by 73 nucleotides RPSI07_RS18800 and RPSI07_RS18805 are separated by 135 nucleotides
RPSI07_RS18790: RPSI07_RS18790 - TetR/AcrR family transcriptional regulator, at 2,410,353 to 2,410,931
_RS18790
RPSI07_RS18795: RPSI07_RS18795 - XRE family transcriptional regulator, at 2,411,035 to 2,411,376
_RS18795
RPSI07_RS18800: RPSI07_RS18800 - AEC family transporter, at 2,411,450 to 2,412,382
_RS18800
RPSI07_RS18805: RPSI07_RS18805 - LysR family transcriptional regulator, at 2,412,518 to 2,413,417
_RS18805
Position (kb)
2411
2412
2413 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2410.751 kb on + strand, within RPSI07_RS18790 at 2410.864 kb on - strand, within RPSI07_RS18790 at 2410.888 kb on + strand at 2410.889 kb on - strand at 2410.929 kb on + strand at 2410.930 kb on - strand at 2410.930 kb on - strand at 2411.222 kb on + strand, within RPSI07_RS18795 at 2411.222 kb on + strand, within RPSI07_RS18795 at 2411.222 kb on + strand, within RPSI07_RS18795 at 2411.222 kb on + strand, within RPSI07_RS18795 at 2411.223 kb on - strand, within RPSI07_RS18795 at 2411.223 kb on - strand, within RPSI07_RS18795 at 2411.223 kb on - strand, within RPSI07_RS18795 at 2411.223 kb on - strand, within RPSI07_RS18795 at 2411.580 kb on + strand, within RPSI07_RS18800 at 2411.581 kb on - strand, within RPSI07_RS18800 at 2411.976 kb on + strand, within RPSI07_RS18800 at 2412.060 kb on + strand at 2412.060 kb on + strand, within RPSI07_RS18800 at 2412.060 kb on + strand, within RPSI07_RS18800 at 2412.060 kb on + strand, within RPSI07_RS18800 at 2412.061 kb on - strand, within RPSI07_RS18800 at 2412.061 kb on - strand, within RPSI07_RS18800 at 2412.090 kb on - strand, within RPSI07_RS18800 at 2412.090 kb on - strand, within RPSI07_RS18800 at 2412.258 kb on + strand, within RPSI07_RS18800 at 2412.258 kb on + strand, within RPSI07_RS18800 at 2412.258 kb on + strand, within RPSI07_RS18800 at 2412.258 kb on + strand, within RPSI07_RS18800 at 2412.258 kb on + strand, within RPSI07_RS18800 at 2412.258 kb on + strand, within RPSI07_RS18800 at 2412.258 kb on + strand, within RPSI07_RS18800 at 2412.259 kb on - strand, within RPSI07_RS18800 at 2412.259 kb on - strand, within RPSI07_RS18800 at 2412.259 kb on - strand, within RPSI07_RS18800 at 2412.259 kb on - strand, within RPSI07_RS18800 at 2412.261 kb on + strand, within RPSI07_RS18800 at 2412.261 kb on + strand, within RPSI07_RS18800 at 2412.261 kb on + strand, within RPSI07_RS18800 at 2412.261 kb on + strand, within RPSI07_RS18800 at 2412.261 kb on + strand, within RPSI07_RS18800 at 2412.262 kb on - strand, within RPSI07_RS18800 at 2412.262 kb on - strand, within RPSI07_RS18800 at 2412.262 kb on - strand, within RPSI07_RS18800 at 2412.262 kb on - strand, within RPSI07_RS18800 at 2412.262 kb on - strand, within RPSI07_RS18800 at 2412.262 kb on - strand, within RPSI07_RS18800 at 2412.490 kb on + strand at 2412.490 kb on + strand at 2412.490 kb on + strand at 2412.497 kb on + strand at 2412.498 kb on - strand at 2412.503 kb on + strand at 2412.504 kb on - strand at 2412.504 kb on - strand at 2412.504 kb on - strand at 2412.512 kb on + strand at 2412.512 kb on + strand at 2412.933 kb on + strand, within RPSI07_RS18805 at 2412.933 kb on + strand, within RPSI07_RS18805 at 2413.194 kb on + strand, within RPSI07_RS18805 at 2413.195 kb on - strand, within RPSI07_RS18805 at 2413.195 kb on - strand, within RPSI07_RS18805
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 2,410,751 + RPSI07_RS18790 0.69 -0.3 2,410,864 - RPSI07_RS18790 0.88 -0.8 2,410,888 + +1.1 2,410,889 - -0.4 2,410,929 + -0.5 2,410,930 - -1.3 2,410,930 - -1.8 2,411,222 + RPSI07_RS18795 0.55 -0.5 2,411,222 + RPSI07_RS18795 0.55 +1.7 2,411,222 + RPSI07_RS18795 0.55 +1.1 2,411,222 + RPSI07_RS18795 0.55 +2.7 2,411,223 - RPSI07_RS18795 0.55 +1.1 2,411,223 - RPSI07_RS18795 0.55 -1.3 2,411,223 - RPSI07_RS18795 0.55 +0.1 2,411,223 - RPSI07_RS18795 0.55 +0.1 2,411,580 + RPSI07_RS18800 0.14 -0.4 2,411,581 - RPSI07_RS18800 0.14 +0.3 2,411,976 + RPSI07_RS18800 0.56 -0.1 2,412,060 + -3.8 2,412,060 + RPSI07_RS18800 0.65 +0.1 2,412,060 + RPSI07_RS18800 0.65 +2.7 2,412,060 + RPSI07_RS18800 0.65 +0.3 2,412,061 - RPSI07_RS18800 0.65 -0.3 2,412,061 - RPSI07_RS18800 0.65 -0.5 2,412,090 - RPSI07_RS18800 0.69 -0.7 2,412,090 - RPSI07_RS18800 0.69 -0.4 2,412,258 + RPSI07_RS18800 0.87 -2.3 2,412,258 + RPSI07_RS18800 0.87 -1.0 2,412,258 + RPSI07_RS18800 0.87 +0.8 2,412,258 + RPSI07_RS18800 0.87 +2.3 2,412,258 + RPSI07_RS18800 0.87 +0.1 2,412,258 + RPSI07_RS18800 0.87 -0.4 2,412,258 + RPSI07_RS18800 0.87 +1.7 2,412,259 - RPSI07_RS18800 0.87 +1.1 2,412,259 - RPSI07_RS18800 0.87 +0.3 2,412,259 - RPSI07_RS18800 0.87 +0.9 2,412,259 - RPSI07_RS18800 0.87 -1.4 2,412,261 + RPSI07_RS18800 0.87 -2.0 2,412,261 + RPSI07_RS18800 0.87 +0.9 2,412,261 + RPSI07_RS18800 0.87 -0.5 2,412,261 + RPSI07_RS18800 0.87 -0.4 2,412,261 + RPSI07_RS18800 0.87 -0.4 2,412,262 - RPSI07_RS18800 0.87 +1.1 2,412,262 - RPSI07_RS18800 0.87 -0.5 2,412,262 - RPSI07_RS18800 0.87 +1.0 2,412,262 - RPSI07_RS18800 0.87 +0.9 2,412,262 - RPSI07_RS18800 0.87 +0.5 2,412,262 - RPSI07_RS18800 0.87 +1.9 2,412,490 + -1.6 2,412,490 + -1.9 2,412,490 + +1.1 2,412,497 + -1.1 2,412,498 - -0.9 2,412,503 + +0.1 2,412,504 - +0.7 2,412,504 - +1.3 2,412,504 - +1.4 2,412,512 + +0.6 2,412,512 + +0.1 2,412,933 + RPSI07_RS18805 0.46 +0.1 2,412,933 + RPSI07_RS18805 0.46 -0.5 2,413,194 + RPSI07_RS18805 0.75 -1.4 2,413,195 - RPSI07_RS18805 0.75 +1.1 2,413,195 - RPSI07_RS18805 0.75 +0.8
Or see this region's nucleotide sequence