Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS14500

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS14490 and RPSI07_RS14495 overlap by 50 nucleotidesRPSI07_RS14495 and RPSI07_RS14500 are separated by 8 nucleotides RPSI07_RS14490: RPSI07_RS14490 - urease accessory protein UreE, at 1,431,009 to 1,431,632 _RS14490 RPSI07_RS14495: RPSI07_RS14495 - urease accessory protein UreF, at 1,431,583 to 1,432,314 _RS14495 RPSI07_RS14500: RPSI07_RS14500 - urease accessory protein UreG, at 1,432,323 to 1,432,955 _RS14500 Position (kb) 1432 1433Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1431.442 kb on - strand, within RPSI07_RS14490at 1431.489 kb on + strand, within RPSI07_RS14490at 1431.490 kb on - strand, within RPSI07_RS14490at 1431.511 kb on - strand, within RPSI07_RS14490at 1431.631 kb on - strandat 1431.697 kb on + strand, within RPSI07_RS14495at 1431.697 kb on + strand, within RPSI07_RS14495at 1431.697 kb on + strand, within RPSI07_RS14495at 1432.082 kb on - strand, within RPSI07_RS14495at 1432.291 kb on + strandat 1432.291 kb on + strandat 1432.292 kb on - strandat 1432.292 kb on - strandat 1432.292 kb on - strandat 1432.292 kb on - strandat 1432.329 kb on - strandat 1432.329 kb on - strandat 1432.329 kb on - strandat 1432.428 kb on + strand, within RPSI07_RS14500at 1432.428 kb on + strand, within RPSI07_RS14500at 1432.429 kb on - strand, within RPSI07_RS14500at 1432.458 kb on + strand, within RPSI07_RS14500at 1432.458 kb on + strand, within RPSI07_RS14500at 1432.458 kb on + strand, within RPSI07_RS14500at 1432.459 kb on - strand, within RPSI07_RS14500at 1432.459 kb on - strand, within RPSI07_RS14500at 1432.459 kb on - strand, within RPSI07_RS14500at 1432.459 kb on - strand, within RPSI07_RS14500at 1432.459 kb on - strand, within RPSI07_RS14500at 1432.459 kb on - strand, within RPSI07_RS14500at 1432.459 kb on - strand, within RPSI07_RS14500at 1432.695 kb on + strand, within RPSI07_RS14500at 1432.695 kb on + strand, within RPSI07_RS14500at 1432.695 kb on + strand, within RPSI07_RS14500at 1432.695 kb on + strand, within RPSI07_RS14500at 1432.695 kb on + strand, within RPSI07_RS14500at 1432.696 kb on - strand, within RPSI07_RS14500at 1432.696 kb on - strand, within RPSI07_RS14500at 1432.696 kb on - strand, within RPSI07_RS14500at 1432.797 kb on + strand, within RPSI07_RS14500at 1432.797 kb on + strand, within RPSI07_RS14500at 1433.256 kb on + strandat 1433.257 kb on - strandat 1433.284 kb on + strandat 1433.285 kb on - strandat 1433.285 kb on - strandat 1433.285 kb on - strandat 1433.332 kb on - strandat 1433.387 kb on - strandat 1433.387 kb on - strandat 1433.416 kb on + strandat 1433.416 kb on + strandat 1433.515 kb on + strandat 1433.515 kb on + strandat 1433.515 kb on + strandat 1433.516 kb on - strandat 1433.516 kb on - strandat 1433.516 kb on - strandat 1433.528 kb on + strandat 1433.660 kb on + strandat 1433.769 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
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1,431,442 - RPSI07_RS14490 0.69 -0.8
1,431,489 + RPSI07_RS14490 0.77 +2.0
1,431,490 - RPSI07_RS14490 0.77 +2.0
1,431,511 - RPSI07_RS14490 0.80 -1.0
1,431,631 - -1.0
1,431,697 + RPSI07_RS14495 0.16 +0.8
1,431,697 + RPSI07_RS14495 0.16 +2.4
1,431,697 + RPSI07_RS14495 0.16 -0.6
1,432,082 - RPSI07_RS14495 0.68 -1.4
1,432,291 + -0.8
1,432,291 + -0.8
1,432,292 - +0.6
1,432,292 - +2.4
1,432,292 - -1.5
1,432,292 - +0.5
1,432,329 - -0.8
1,432,329 - +0.3
1,432,329 - -0.4
1,432,428 + RPSI07_RS14500 0.17 -0.1
1,432,428 + RPSI07_RS14500 0.17 -1.3
1,432,429 - RPSI07_RS14500 0.17 +0.4
1,432,458 + RPSI07_RS14500 0.21 -1.5
1,432,458 + RPSI07_RS14500 0.21 +0.8
1,432,458 + RPSI07_RS14500 0.21 -0.2
1,432,459 - RPSI07_RS14500 0.21 -0.4
1,432,459 - RPSI07_RS14500 0.21 +1.6
1,432,459 - RPSI07_RS14500 0.21 -1.1
1,432,459 - RPSI07_RS14500 0.21 +1.1
1,432,459 - RPSI07_RS14500 0.21 -0.1
1,432,459 - RPSI07_RS14500 0.21 +2.8
1,432,459 - RPSI07_RS14500 0.21 -1.0
1,432,695 + RPSI07_RS14500 0.59 -0.6
1,432,695 + RPSI07_RS14500 0.59 +0.2
1,432,695 + RPSI07_RS14500 0.59 -0.1
1,432,695 + RPSI07_RS14500 0.59 +3.6
1,432,695 + RPSI07_RS14500 0.59 -0.9
1,432,696 - RPSI07_RS14500 0.59 -0.7
1,432,696 - RPSI07_RS14500 0.59 -0.3
1,432,696 - RPSI07_RS14500 0.59 +1.5
1,432,797 + RPSI07_RS14500 0.75 +3.5
1,432,797 + RPSI07_RS14500 0.75 -1.8
1,433,256 + -2.1
1,433,257 - +0.4
1,433,284 + +2.0
1,433,285 - -1.9
1,433,285 - +0.0
1,433,285 - -0.9
1,433,332 - +0.5
1,433,387 - -0.5
1,433,387 - +0.0
1,433,416 + -1.0
1,433,416 + -0.4
1,433,515 + -0.5
1,433,515 + +0.7
1,433,515 + -1.0
1,433,516 - +0.3
1,433,516 - +0.4
1,433,516 - +0.8
1,433,528 + +0.3
1,433,660 + -1.3
1,433,769 - +0.7

Or see this region's nucleotide sequence