Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS14490 and RPSI07_RS14495 overlap by 50 nucleotides RPSI07_RS14495 and RPSI07_RS14500 are separated by 8 nucleotides
RPSI07_RS14490: RPSI07_RS14490 - urease accessory protein UreE, at 1,431,009 to 1,431,632
_RS14490
RPSI07_RS14495: RPSI07_RS14495 - urease accessory protein UreF, at 1,431,583 to 1,432,314
_RS14495
RPSI07_RS14500: RPSI07_RS14500 - urease accessory protein UreG, at 1,432,323 to 1,432,955
_RS14500
Position (kb)
1432
1433 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1431.442 kb on - strand, within RPSI07_RS14490 at 1431.489 kb on + strand, within RPSI07_RS14490 at 1431.490 kb on - strand, within RPSI07_RS14490 at 1431.511 kb on - strand, within RPSI07_RS14490 at 1431.631 kb on - strand at 1431.697 kb on + strand, within RPSI07_RS14495 at 1431.697 kb on + strand, within RPSI07_RS14495 at 1431.697 kb on + strand, within RPSI07_RS14495 at 1432.082 kb on - strand, within RPSI07_RS14495 at 1432.291 kb on + strand at 1432.291 kb on + strand at 1432.292 kb on - strand at 1432.292 kb on - strand at 1432.292 kb on - strand at 1432.292 kb on - strand at 1432.329 kb on - strand at 1432.329 kb on - strand at 1432.329 kb on - strand at 1432.428 kb on + strand, within RPSI07_RS14500 at 1432.428 kb on + strand, within RPSI07_RS14500 at 1432.429 kb on - strand, within RPSI07_RS14500 at 1432.458 kb on + strand, within RPSI07_RS14500 at 1432.458 kb on + strand, within RPSI07_RS14500 at 1432.458 kb on + strand, within RPSI07_RS14500 at 1432.459 kb on - strand, within RPSI07_RS14500 at 1432.459 kb on - strand, within RPSI07_RS14500 at 1432.459 kb on - strand, within RPSI07_RS14500 at 1432.459 kb on - strand, within RPSI07_RS14500 at 1432.459 kb on - strand, within RPSI07_RS14500 at 1432.459 kb on - strand, within RPSI07_RS14500 at 1432.459 kb on - strand, within RPSI07_RS14500 at 1432.695 kb on + strand, within RPSI07_RS14500 at 1432.695 kb on + strand, within RPSI07_RS14500 at 1432.695 kb on + strand, within RPSI07_RS14500 at 1432.695 kb on + strand, within RPSI07_RS14500 at 1432.695 kb on + strand, within RPSI07_RS14500 at 1432.696 kb on - strand, within RPSI07_RS14500 at 1432.696 kb on - strand, within RPSI07_RS14500 at 1432.696 kb on - strand, within RPSI07_RS14500 at 1432.797 kb on + strand, within RPSI07_RS14500 at 1432.797 kb on + strand, within RPSI07_RS14500 at 1433.256 kb on + strand at 1433.257 kb on - strand at 1433.284 kb on + strand at 1433.285 kb on - strand at 1433.285 kb on - strand at 1433.285 kb on - strand at 1433.332 kb on - strand at 1433.387 kb on - strand at 1433.387 kb on - strand at 1433.416 kb on + strand at 1433.416 kb on + strand at 1433.515 kb on + strand at 1433.515 kb on + strand at 1433.515 kb on + strand at 1433.516 kb on - strand at 1433.516 kb on - strand at 1433.516 kb on - strand at 1433.528 kb on + strand at 1433.660 kb on + strand at 1433.769 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 1,431,442 - RPSI07_RS14490 0.69 -0.8 1,431,489 + RPSI07_RS14490 0.77 +2.0 1,431,490 - RPSI07_RS14490 0.77 +2.0 1,431,511 - RPSI07_RS14490 0.80 -1.0 1,431,631 - -1.0 1,431,697 + RPSI07_RS14495 0.16 +0.8 1,431,697 + RPSI07_RS14495 0.16 +2.4 1,431,697 + RPSI07_RS14495 0.16 -0.6 1,432,082 - RPSI07_RS14495 0.68 -1.4 1,432,291 + -0.8 1,432,291 + -0.8 1,432,292 - +0.6 1,432,292 - +2.4 1,432,292 - -1.5 1,432,292 - +0.5 1,432,329 - -0.8 1,432,329 - +0.3 1,432,329 - -0.4 1,432,428 + RPSI07_RS14500 0.17 -0.1 1,432,428 + RPSI07_RS14500 0.17 -1.3 1,432,429 - RPSI07_RS14500 0.17 +0.4 1,432,458 + RPSI07_RS14500 0.21 -1.5 1,432,458 + RPSI07_RS14500 0.21 +0.8 1,432,458 + RPSI07_RS14500 0.21 -0.2 1,432,459 - RPSI07_RS14500 0.21 -0.4 1,432,459 - RPSI07_RS14500 0.21 +1.6 1,432,459 - RPSI07_RS14500 0.21 -1.1 1,432,459 - RPSI07_RS14500 0.21 +1.1 1,432,459 - RPSI07_RS14500 0.21 -0.1 1,432,459 - RPSI07_RS14500 0.21 +2.8 1,432,459 - RPSI07_RS14500 0.21 -1.0 1,432,695 + RPSI07_RS14500 0.59 -0.6 1,432,695 + RPSI07_RS14500 0.59 +0.2 1,432,695 + RPSI07_RS14500 0.59 -0.1 1,432,695 + RPSI07_RS14500 0.59 +3.6 1,432,695 + RPSI07_RS14500 0.59 -0.9 1,432,696 - RPSI07_RS14500 0.59 -0.7 1,432,696 - RPSI07_RS14500 0.59 -0.3 1,432,696 - RPSI07_RS14500 0.59 +1.5 1,432,797 + RPSI07_RS14500 0.75 +3.5 1,432,797 + RPSI07_RS14500 0.75 -1.8 1,433,256 + -2.1 1,433,257 - +0.4 1,433,284 + +2.0 1,433,285 - -1.9 1,433,285 - +0.0 1,433,285 - -0.9 1,433,332 - +0.5 1,433,387 - -0.5 1,433,387 - +0.0 1,433,416 + -1.0 1,433,416 + -0.4 1,433,515 + -0.5 1,433,515 + +0.7 1,433,515 + -1.0 1,433,516 - +0.3 1,433,516 - +0.4 1,433,516 - +0.8 1,433,528 + +0.3 1,433,660 + -1.3 1,433,769 - +0.7
Or see this region's nucleotide sequence