Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS11670 and RPSI07_RS11675 are separated by 37 nucleotides RPSI07_RS11675 and RPSI07_RS11680 are separated by 248 nucleotides RPSI07_RS11680 and RPSI07_RS11685 are separated by 452 nucleotides RPSI07_RS11685 and RPSI07_RS24985 overlap by 62 nucleotides
RPSI07_RS11670: RPSI07_RS11670 - calcium-binding protein, at 825,873 to 826,841
_RS11670
RPSI07_RS11675: RPSI07_RS11675 - chromate resistance protein, at 826,879 to 827,337
_RS11675
RPSI07_RS11680: RPSI07_RS11680 - hypothetical protein, at 827,586 to 828,520
_RS11680
RPSI07_RS11685: RPSI07_RS11685 - istB-like ATP-binding protein domain, at 828,973 to 829,257
_RS11685
RPSI07_RS24985: RPSI07_RS24985 - hypothetical protein, at 829,196 to 829,565
_RS24985
Position (kb) 827
828
829 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 826.899 kb on + strand at 826.899 kb on + strand at 826.969 kb on + strand, within RPSI07_RS11675 at 826.969 kb on + strand, within RPSI07_RS11675 at 826.969 kb on + strand, within RPSI07_RS11675 at 826.970 kb on - strand, within RPSI07_RS11675 at 826.970 kb on - strand, within RPSI07_RS11675 at 827.093 kb on - strand, within RPSI07_RS11675 at 827.093 kb on - strand, within RPSI07_RS11675 at 827.093 kb on - strand, within RPSI07_RS11675 at 827.093 kb on - strand, within RPSI07_RS11675 at 827.151 kb on + strand, within RPSI07_RS11675 at 827.152 kb on - strand, within RPSI07_RS11675 at 827.152 kb on - strand, within RPSI07_RS11675 at 827.191 kb on + strand, within RPSI07_RS11675 at 827.192 kb on - strand, within RPSI07_RS11675 at 827.263 kb on + strand, within RPSI07_RS11675 at 827.263 kb on + strand, within RPSI07_RS11675 at 827.263 kb on + strand, within RPSI07_RS11675 at 827.263 kb on + strand, within RPSI07_RS11675 at 827.263 kb on + strand, within RPSI07_RS11675 at 827.264 kb on - strand, within RPSI07_RS11675 at 827.275 kb on + strand, within RPSI07_RS11675 at 827.275 kb on + strand, within RPSI07_RS11675 at 827.275 kb on + strand, within RPSI07_RS11675 at 827.275 kb on + strand, within RPSI07_RS11675 at 827.275 kb on + strand, within RPSI07_RS11675 at 827.276 kb on - strand, within RPSI07_RS11675 at 827.276 kb on - strand, within RPSI07_RS11675 at 827.276 kb on - strand at 827.400 kb on + strand at 827.421 kb on + strand at 827.421 kb on + strand at 827.421 kb on + strand at 827.422 kb on - strand at 827.422 kb on - strand at 827.422 kb on - strand at 827.422 kb on - strand at 827.424 kb on + strand at 827.425 kb on - strand at 827.425 kb on - strand at 827.434 kb on + strand at 827.434 kb on + strand at 827.434 kb on + strand at 827.434 kb on + strand at 827.434 kb on + strand at 827.434 kb on + strand at 827.435 kb on - strand at 827.435 kb on - strand at 827.435 kb on - strand at 827.514 kb on - strand at 827.793 kb on - strand, within RPSI07_RS11680 at 827.867 kb on + strand, within RPSI07_RS11680 at 827.867 kb on + strand at 827.868 kb on - strand, within RPSI07_RS11680 at 827.868 kb on - strand, within RPSI07_RS11680 at 827.868 kb on - strand, within RPSI07_RS11680 at 827.878 kb on + strand, within RPSI07_RS11680 at 827.878 kb on + strand, within RPSI07_RS11680 at 827.878 kb on + strand, within RPSI07_RS11680 at 827.878 kb on + strand, within RPSI07_RS11680 at 827.879 kb on - strand, within RPSI07_RS11680 at 827.879 kb on - strand, within RPSI07_RS11680 at 827.879 kb on - strand, within RPSI07_RS11680 at 827.965 kb on + strand, within RPSI07_RS11680 at 827.965 kb on + strand, within RPSI07_RS11680 at 827.965 kb on + strand, within RPSI07_RS11680 at 827.966 kb on - strand, within RPSI07_RS11680 at 827.966 kb on - strand, within RPSI07_RS11680 at 827.966 kb on - strand, within RPSI07_RS11680 at 827.966 kb on - strand, within RPSI07_RS11680 at 827.966 kb on - strand, within RPSI07_RS11680 at 827.989 kb on + strand, within RPSI07_RS11680 at 827.989 kb on + strand, within RPSI07_RS11680 at 827.990 kb on - strand, within RPSI07_RS11680 at 828.060 kb on - strand, within RPSI07_RS11680 at 828.251 kb on + strand, within RPSI07_RS11680 at 828.252 kb on - strand, within RPSI07_RS11680 at 828.258 kb on - strand, within RPSI07_RS11680 at 828.275 kb on - strand, within RPSI07_RS11680 at 828.318 kb on + strand, within RPSI07_RS11680 at 828.319 kb on - strand, within RPSI07_RS11680 at 828.319 kb on - strand, within RPSI07_RS11680 at 828.319 kb on - strand, within RPSI07_RS11680 at 828.622 kb on + strand at 828.622 kb on + strand at 828.622 kb on + strand at 828.622 kb on + strand at 828.622 kb on + strand at 828.622 kb on + strand at 828.622 kb on + strand at 828.622 kb on + strand at 828.623 kb on - strand at 828.623 kb on - strand at 828.623 kb on - strand at 828.623 kb on - strand at 828.623 kb on - strand at 828.623 kb on - strand at 828.624 kb on - strand at 828.643 kb on + strand at 828.643 kb on + strand at 828.643 kb on + strand at 828.643 kb on + strand at 828.644 kb on - strand at 828.644 kb on - strand at 828.644 kb on - strand at 828.644 kb on - strand at 828.655 kb on + strand at 828.656 kb on - strand at 828.656 kb on - strand at 828.885 kb on + strand at 828.885 kb on + strand at 828.885 kb on + strand at 828.886 kb on - strand at 828.957 kb on + strand at 828.979 kb on + strand at 828.993 kb on + strand at 828.997 kb on + strand at 828.997 kb on + strand at 828.997 kb on + strand at 828.997 kb on + strand at 828.997 kb on + strand at 828.997 kb on + strand at 828.998 kb on - strand at 828.998 kb on - strand at 828.998 kb on - strand at 829.250 kb on + strand at 829.250 kb on + strand at 829.251 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 826,899 + -0.6 826,899 + +1.2 826,969 + RPSI07_RS11675 0.20 -1.0 826,969 + RPSI07_RS11675 0.20 -0.8 826,969 + RPSI07_RS11675 0.20 -1.4 826,970 - RPSI07_RS11675 0.20 +0.6 826,970 - RPSI07_RS11675 0.20 -1.1 827,093 - RPSI07_RS11675 0.47 +1.2 827,093 - RPSI07_RS11675 0.47 -1.0 827,093 - RPSI07_RS11675 0.47 -0.4 827,093 - RPSI07_RS11675 0.47 +0.4 827,151 + RPSI07_RS11675 0.59 -0.3 827,152 - RPSI07_RS11675 0.59 -1.4 827,152 - RPSI07_RS11675 0.59 +1.8 827,191 + RPSI07_RS11675 0.68 +0.3 827,192 - RPSI07_RS11675 0.68 -0.5 827,263 + RPSI07_RS11675 0.84 +0.3 827,263 + RPSI07_RS11675 0.84 -2.0 827,263 + RPSI07_RS11675 0.84 -0.8 827,263 + RPSI07_RS11675 0.84 +2.2 827,263 + RPSI07_RS11675 0.84 -0.0 827,264 - RPSI07_RS11675 0.84 +0.6 827,275 + RPSI07_RS11675 0.86 -0.7 827,275 + RPSI07_RS11675 0.86 -0.7 827,275 + RPSI07_RS11675 0.86 -0.8 827,275 + RPSI07_RS11675 0.86 -1.4 827,275 + RPSI07_RS11675 0.86 -0.4 827,276 - RPSI07_RS11675 0.86 +0.2 827,276 - RPSI07_RS11675 0.86 -3.2 827,276 - -0.4 827,400 + +0.2 827,421 + -0.5 827,421 + -0.5 827,421 + -0.3 827,422 - +1.6 827,422 - -0.6 827,422 - -1.2 827,422 - -0.5 827,424 + +1.6 827,425 - +1.2 827,425 - -0.3 827,434 + -1.1 827,434 + -0.3 827,434 + -0.4 827,434 + -1.4 827,434 + -1.6 827,434 + +1.6 827,435 - +0.5 827,435 - +1.7 827,435 - -1.2 827,514 - +1.0 827,793 - RPSI07_RS11680 0.22 +0.9 827,867 + RPSI07_RS11680 0.30 +2.2 827,867 + +0.2 827,868 - RPSI07_RS11680 0.30 +2.5 827,868 - RPSI07_RS11680 0.30 +0.2 827,868 - RPSI07_RS11680 0.30 -0.1 827,878 + RPSI07_RS11680 0.31 -0.0 827,878 + RPSI07_RS11680 0.31 +0.2 827,878 + RPSI07_RS11680 0.31 +1.5 827,878 + RPSI07_RS11680 0.31 -0.6 827,879 - RPSI07_RS11680 0.31 -0.1 827,879 - RPSI07_RS11680 0.31 +1.2 827,879 - RPSI07_RS11680 0.31 +0.8 827,965 + RPSI07_RS11680 0.41 -0.7 827,965 + RPSI07_RS11680 0.41 -2.7 827,965 + RPSI07_RS11680 0.41 +2.1 827,966 - RPSI07_RS11680 0.41 -0.8 827,966 - RPSI07_RS11680 0.41 -0.9 827,966 - RPSI07_RS11680 0.41 +2.7 827,966 - RPSI07_RS11680 0.41 -1.3 827,966 - RPSI07_RS11680 0.41 -3.7 827,989 + RPSI07_RS11680 0.43 +2.2 827,989 + RPSI07_RS11680 0.43 +1.0 827,990 - RPSI07_RS11680 0.43 -1.7 828,060 - RPSI07_RS11680 0.51 -0.0 828,251 + RPSI07_RS11680 0.71 -0.3 828,252 - RPSI07_RS11680 0.71 -1.2 828,258 - RPSI07_RS11680 0.72 +1.2 828,275 - RPSI07_RS11680 0.74 -0.0 828,318 + RPSI07_RS11680 0.78 +0.6 828,319 - RPSI07_RS11680 0.78 +1.2 828,319 - RPSI07_RS11680 0.78 +2.7 828,319 - RPSI07_RS11680 0.78 -0.2 828,622 + +3.3 828,622 + +0.6 828,622 + -0.7 828,622 + +0.8 828,622 + -1.4 828,622 + -1.8 828,622 + -1.8 828,622 + -0.9 828,623 - +3.5 828,623 - +0.4 828,623 - +1.3 828,623 - +0.2 828,623 - -1.2 828,623 - +3.5 828,624 - -1.7 828,643 + -0.6 828,643 + -1.7 828,643 + -0.4 828,643 + +2.2 828,644 - -0.6 828,644 - -1.3 828,644 - -0.4 828,644 - -1.4 828,655 + -0.7 828,656 - -0.8 828,656 - -0.1 828,885 + +1.2 828,885 + -2.3 828,885 + +0.4 828,886 - +0.1 828,957 + +1.9 828,979 + +0.5 828,993 + +0.5 828,997 + -0.8 828,997 + -1.2 828,997 + -0.5 828,997 + +0.2 828,997 + +0.9 828,997 + -0.9 828,998 - +2.0 828,998 - -1.7 828,998 - -0.4 829,250 + -3.8 829,250 + -1.0 829,251 - +2.7
Or see this region's nucleotide sequence