Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10550 and RPSI07_RS10555 are separated by 85 nucleotides RPSI07_RS10555 and RPSI07_RS10560 are separated by 2 nucleotides RPSI07_RS10560 and RPSI07_RS10565 are separated by 575 nucleotides
RPSI07_RS10550: RPSI07_RS10550 - TonB-dependent siderophore receptor, at 569,398 to 571,803
_RS10550
RPSI07_RS10555: RPSI07_RS10555 - FecR family protein, at 571,889 to 572,713
_RS10555
RPSI07_RS10560: RPSI07_RS10560 - RNA polymerase sigma factor, at 572,716 to 573,273
_RS10560
RPSI07_RS10565: RPSI07_RS10565 - ubiquinol oxidase subunit II, at 573,849 to 574,835
_RS10565
Position (kb) 572
573
574 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 571.828 kb on + strand at 571.828 kb on + strand at 571.843 kb on + strand at 571.843 kb on + strand at 571.843 kb on + strand at 571.869 kb on + strand at 571.870 kb on - strand at 571.870 kb on - strand at 572.518 kb on - strand, within RPSI07_RS10555 at 572.518 kb on - strand, within RPSI07_RS10555 at 573.056 kb on + strand, within RPSI07_RS10560 at 573.056 kb on + strand, within RPSI07_RS10560 at 573.057 kb on - strand, within RPSI07_RS10560 at 573.057 kb on - strand, within RPSI07_RS10560 at 573.188 kb on + strand, within RPSI07_RS10560 at 573.188 kb on + strand, within RPSI07_RS10560 at 573.188 kb on + strand, within RPSI07_RS10560 at 573.189 kb on - strand, within RPSI07_RS10560 at 573.559 kb on + strand at 573.559 kb on + strand at 573.559 kb on + strand at 574.167 kb on + strand, within RPSI07_RS10565
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 571,828 + -0.4 571,828 + +4.2 571,843 + -1.1 571,843 + +1.8 571,843 + +0.2 571,869 + -2.1 571,870 - +2.5 571,870 - -0.9 572,518 - RPSI07_RS10555 0.76 +0.6 572,518 - RPSI07_RS10555 0.76 +1.7 573,056 + RPSI07_RS10560 0.61 +2.2 573,056 + RPSI07_RS10560 0.61 -1.2 573,057 - RPSI07_RS10560 0.61 +0.2 573,057 - RPSI07_RS10560 0.61 -3.7 573,188 + RPSI07_RS10560 0.85 +0.7 573,188 + RPSI07_RS10560 0.85 -0.6 573,188 + RPSI07_RS10560 0.85 +0.0 573,189 - RPSI07_RS10560 0.85 -1.9 573,559 + -0.1 573,559 + +1.5 573,559 + -0.7 574,167 + RPSI07_RS10565 0.32 +0.2
Or see this region's nucleotide sequence