Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS05590 and RPSI07_RS05595 are separated by 376 nucleotides RPSI07_RS05595 and RPSI07_RS05600 are separated by 22 nucleotides
RPSI07_RS05590: RPSI07_RS05590 - DUF2239 domain-containing protein, at 1,521,887 to 1,522,534
_RS05590
RPSI07_RS05595: RPSI07_RS05595 - nitrate reductase, at 1,522,911 to 1,525,652
_RS05595
RPSI07_RS05600: RPSI07_RS05600 - NAD(P)/FAD-dependent oxidoreductase, at 1,525,675 to 1,526,904
_RS05600
Position (kb)
1522
1523
1524
1525
1526 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1522.538 kb on + strand at 1522.538 kb on + strand at 1522.538 kb on + strand at 1522.604 kb on + strand at 1522.605 kb on - strand at 1522.616 kb on + strand at 1522.617 kb on - strand at 1522.651 kb on - strand at 1522.695 kb on - strand at 1523.383 kb on + strand, within RPSI07_RS05595 at 1523.384 kb on - strand, within RPSI07_RS05595 at 1523.839 kb on + strand, within RPSI07_RS05595 at 1523.992 kb on + strand, within RPSI07_RS05595 at 1524.127 kb on + strand, within RPSI07_RS05595 at 1524.127 kb on + strand, within RPSI07_RS05595 at 1524.741 kb on - strand, within RPSI07_RS05595 at 1524.757 kb on + strand, within RPSI07_RS05595 at 1524.758 kb on - strand, within RPSI07_RS05595 at 1524.758 kb on - strand, within RPSI07_RS05595 at 1525.132 kb on + strand, within RPSI07_RS05595 at 1525.133 kb on - strand, within RPSI07_RS05595 at 1525.261 kb on + strand, within RPSI07_RS05595 at 1525.261 kb on + strand, within RPSI07_RS05595 at 1525.261 kb on + strand, within RPSI07_RS05595 at 1525.261 kb on + strand, within RPSI07_RS05595 at 1525.262 kb on - strand, within RPSI07_RS05595 at 1525.262 kb on - strand, within RPSI07_RS05595 at 1525.262 kb on - strand, within RPSI07_RS05595 at 1525.264 kb on + strand, within RPSI07_RS05595 at 1525.264 kb on + strand, within RPSI07_RS05595 at 1525.264 kb on + strand, within RPSI07_RS05595 at 1525.265 kb on - strand, within RPSI07_RS05595 at 1525.265 kb on - strand, within RPSI07_RS05595 at 1525.265 kb on - strand, within RPSI07_RS05595 at 1525.294 kb on + strand, within RPSI07_RS05595 at 1525.294 kb on + strand, within RPSI07_RS05595 at 1525.294 kb on + strand, within RPSI07_RS05595 at 1525.294 kb on + strand, within RPSI07_RS05595 at 1525.295 kb on - strand, within RPSI07_RS05595 at 1525.439 kb on - strand at 1525.767 kb on - strand at 1525.767 kb on - strand at 1525.767 kb on - strand at 1525.812 kb on + strand, within RPSI07_RS05600 at 1525.812 kb on + strand, within RPSI07_RS05600 at 1525.841 kb on + strand, within RPSI07_RS05600 at 1525.842 kb on - strand, within RPSI07_RS05600 at 1525.964 kb on + strand, within RPSI07_RS05600 at 1525.964 kb on + strand, within RPSI07_RS05600 at 1525.964 kb on + strand, within RPSI07_RS05600 at 1526.078 kb on + strand, within RPSI07_RS05600 at 1526.078 kb on + strand, within RPSI07_RS05600 at 1526.078 kb on + strand, within RPSI07_RS05600 at 1526.078 kb on + strand, within RPSI07_RS05600 at 1526.078 kb on + strand, within RPSI07_RS05600 at 1526.079 kb on - strand, within RPSI07_RS05600 at 1526.093 kb on + strand, within RPSI07_RS05600 at 1526.093 kb on + strand, within RPSI07_RS05600 at 1526.094 kb on - strand, within RPSI07_RS05600 at 1526.591 kb on + strand, within RPSI07_RS05600
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 1,522,538 + -1.5 1,522,538 + -1.4 1,522,538 + +0.1 1,522,604 + -0.2 1,522,605 - +0.5 1,522,616 + +0.9 1,522,617 - -1.7 1,522,651 - -1.0 1,522,695 - +0.2 1,523,383 + RPSI07_RS05595 0.17 -0.7 1,523,384 - RPSI07_RS05595 0.17 -1.4 1,523,839 + RPSI07_RS05595 0.34 -1.0 1,523,992 + RPSI07_RS05595 0.39 -1.8 1,524,127 + RPSI07_RS05595 0.44 +1.7 1,524,127 + RPSI07_RS05595 0.44 +0.1 1,524,741 - RPSI07_RS05595 0.67 +0.1 1,524,757 + RPSI07_RS05595 0.67 +0.9 1,524,758 - RPSI07_RS05595 0.67 -0.1 1,524,758 - RPSI07_RS05595 0.67 -1.8 1,525,132 + RPSI07_RS05595 0.81 -0.7 1,525,133 - RPSI07_RS05595 0.81 -0.3 1,525,261 + RPSI07_RS05595 0.86 -0.6 1,525,261 + RPSI07_RS05595 0.86 -1.7 1,525,261 + RPSI07_RS05595 0.86 -2.0 1,525,261 + RPSI07_RS05595 0.86 -0.1 1,525,262 - RPSI07_RS05595 0.86 +0.9 1,525,262 - RPSI07_RS05595 0.86 -1.6 1,525,262 - RPSI07_RS05595 0.86 +0.8 1,525,264 + RPSI07_RS05595 0.86 -0.0 1,525,264 + RPSI07_RS05595 0.86 -0.9 1,525,264 + RPSI07_RS05595 0.86 -1.0 1,525,265 - RPSI07_RS05595 0.86 -2.7 1,525,265 - RPSI07_RS05595 0.86 -0.7 1,525,265 - RPSI07_RS05595 0.86 +0.6 1,525,294 + RPSI07_RS05595 0.87 -0.4 1,525,294 + RPSI07_RS05595 0.87 +0.0 1,525,294 + RPSI07_RS05595 0.87 +2.9 1,525,294 + RPSI07_RS05595 0.87 +0.2 1,525,295 - RPSI07_RS05595 0.87 -0.6 1,525,439 - -0.2 1,525,767 - +0.1 1,525,767 - +1.1 1,525,767 - -0.7 1,525,812 + RPSI07_RS05600 0.11 +0.9 1,525,812 + RPSI07_RS05600 0.11 +0.7 1,525,841 + RPSI07_RS05600 0.13 +0.2 1,525,842 - RPSI07_RS05600 0.14 +0.9 1,525,964 + RPSI07_RS05600 0.23 +3.9 1,525,964 + RPSI07_RS05600 0.23 -1.7 1,525,964 + RPSI07_RS05600 0.23 -0.8 1,526,078 + RPSI07_RS05600 0.33 -1.2 1,526,078 + RPSI07_RS05600 0.33 -1.0 1,526,078 + RPSI07_RS05600 0.33 +0.3 1,526,078 + RPSI07_RS05600 0.33 -0.7 1,526,078 + RPSI07_RS05600 0.33 +0.3 1,526,079 - RPSI07_RS05600 0.33 -0.7 1,526,093 + RPSI07_RS05600 0.34 +0.1 1,526,093 + RPSI07_RS05600 0.34 -0.7 1,526,094 - RPSI07_RS05600 0.34 -2.1 1,526,591 + RPSI07_RS05600 0.74 -0.3
Or see this region's nucleotide sequence