Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS01795 and RPSI07_RS01800 are separated by 21 nucleotides RPSI07_RS01800 and RPSI07_RS01805 are separated by 68 nucleotides
RPSI07_RS01795: RPSI07_RS01795 - KR domain-containing protein, at 501,403 to 513,870
_RS01795
RPSI07_RS01800: RPSI07_RS01800 - cytochrome P450, at 513,892 to 515,349
_RS01800
RPSI07_RS01805: RPSI07_RS01805 - KR domain-containing protein, at 515,418 to 528,074
_RS01805
Position (kb) 513
514
515
516 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 512.923 kb on + strand at 512.924 kb on - strand at 513.042 kb on + strand at 513.280 kb on + strand at 513.280 kb on + strand at 513.280 kb on + strand at 513.281 kb on - strand at 513.281 kb on - strand at 513.392 kb on - strand at 513.454 kb on + strand at 513.454 kb on + strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.733 kb on + strand at 513.733 kb on + strand at 513.734 kb on - strand at 513.806 kb on - strand at 514.165 kb on + strand, within RPSI07_RS01800 at 514.234 kb on + strand, within RPSI07_RS01800 at 514.234 kb on + strand, within RPSI07_RS01800 at 514.234 kb on + strand, within RPSI07_RS01800 at 514.234 kb on + strand, within RPSI07_RS01800 at 514.234 kb on + strand, within RPSI07_RS01800 at 514.235 kb on - strand, within RPSI07_RS01800 at 514.235 kb on - strand, within RPSI07_RS01800 at 514.235 kb on - strand, within RPSI07_RS01800 at 514.235 kb on - strand, within RPSI07_RS01800 at 514.235 kb on - strand, within RPSI07_RS01800 at 514.235 kb on - strand, within RPSI07_RS01800 at 514.534 kb on + strand, within RPSI07_RS01800 at 514.954 kb on + strand, within RPSI07_RS01800 at 514.955 kb on - strand, within RPSI07_RS01800 at 515.078 kb on - strand, within RPSI07_RS01800 at 515.293 kb on + strand at 515.294 kb on - strand at 515.452 kb on + strand at 515.452 kb on + strand at 515.453 kb on - strand at 515.595 kb on + strand at 515.595 kb on + strand at 515.595 kb on + strand at 515.595 kb on + strand at 515.596 kb on - strand at 515.596 kb on - strand at 515.601 kb on + strand at 515.602 kb on - strand at 515.664 kb on + strand at 515.664 kb on + strand at 515.664 kb on + strand at 515.664 kb on + strand at 515.664 kb on + strand at 515.665 kb on - strand at 515.665 kb on - strand at 515.665 kb on - strand at 515.665 kb on - strand at 515.665 kb on - strand at 516.001 kb on - strand at 516.001 kb on - strand at 516.001 kb on - strand at 516.001 kb on - strand at 516.021 kb on - strand at 516.096 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 512,923 + -0.2 512,924 - +0.5 513,042 + +0.2 513,280 + -0.9 513,280 + -0.1 513,280 + -0.2 513,281 - -0.5 513,281 - +1.9 513,392 - -0.1 513,454 + -0.1 513,454 + +2.9 513,662 - -1.2 513,662 - -1.3 513,662 - +0.6 513,662 - -1.1 513,733 + -1.3 513,733 + +0.7 513,734 - +0.5 513,806 - -0.1 514,165 + RPSI07_RS01800 0.19 -2.0 514,234 + RPSI07_RS01800 0.23 +1.9 514,234 + RPSI07_RS01800 0.23 -0.2 514,234 + RPSI07_RS01800 0.23 +0.5 514,234 + RPSI07_RS01800 0.23 +0.5 514,234 + RPSI07_RS01800 0.23 -0.6 514,235 - RPSI07_RS01800 0.24 -0.5 514,235 - RPSI07_RS01800 0.24 -1.9 514,235 - RPSI07_RS01800 0.24 -1.5 514,235 - RPSI07_RS01800 0.24 +0.3 514,235 - RPSI07_RS01800 0.24 +0.7 514,235 - RPSI07_RS01800 0.24 -0.6 514,534 + RPSI07_RS01800 0.44 +0.9 514,954 + RPSI07_RS01800 0.73 -0.0 514,955 - RPSI07_RS01800 0.73 -1.5 515,078 - RPSI07_RS01800 0.81 -1.4 515,293 + +0.5 515,294 - -0.7 515,452 + +1.9 515,452 + -0.1 515,453 - -0.4 515,595 + +0.9 515,595 + +1.6 515,595 + +0.9 515,595 + -1.4 515,596 - -0.7 515,596 - -0.3 515,601 + -0.5 515,602 - +0.5 515,664 + -1.3 515,664 + -0.1 515,664 + -0.5 515,664 + -0.7 515,664 + +0.2 515,665 - -0.3 515,665 - -0.8 515,665 - -0.5 515,665 - -0.2 515,665 - +0.3 516,001 - -1.6 516,001 - +0.6 516,001 - -0.9 516,001 - -0.1 516,021 - -0.3 516,096 + +0.1
Or see this region's nucleotide sequence