Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS01375 and RPSI07_RS01380 are separated by 115 nucleotides RPSI07_RS01380 and RPSI07_RS01385 are separated by 102 nucleotides
RPSI07_RS01375: RPSI07_RS01375 - flagellar protein FlgN, at 377,749 to 378,210
_RS01375
RPSI07_RS01380: RPSI07_RS01380 - flagellar biosynthesis anti-sigma factor FlgM, at 378,326 to 378,646
_RS01380
RPSI07_RS01385: RPSI07_RS01385 - flagella basal body P-ring formation protein FlgA, at 378,749 to 379,630
_RS01385
Position (kb) 378
379 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 377.394 kb on - strand at 377.489 kb on + strand at 377.534 kb on - strand at 377.534 kb on - strand at 377.573 kb on - strand at 377.573 kb on - strand at 377.615 kb on + strand at 377.615 kb on + strand at 377.615 kb on + strand at 377.616 kb on - strand at 377.620 kb on - strand at 377.750 kb on + strand at 377.750 kb on + strand at 377.750 kb on + strand at 377.808 kb on - strand, within RPSI07_RS01375 at 377.808 kb on - strand, within RPSI07_RS01375 at 377.978 kb on + strand, within RPSI07_RS01375 at 377.978 kb on + strand, within RPSI07_RS01375 at 377.978 kb on + strand, within RPSI07_RS01375 at 377.979 kb on - strand, within RPSI07_RS01375 at 377.979 kb on - strand, within RPSI07_RS01375 at 377.992 kb on + strand, within RPSI07_RS01375 at 377.993 kb on + strand, within RPSI07_RS01375 at 377.993 kb on + strand, within RPSI07_RS01375 at 378.009 kb on + strand, within RPSI07_RS01375 at 378.010 kb on - strand, within RPSI07_RS01375 at 378.186 kb on + strand at 378.291 kb on - strand at 378.291 kb on - strand at 378.291 kb on - strand at 378.674 kb on + strand at 378.674 kb on + strand at 378.674 kb on + strand at 378.674 kb on + strand at 378.674 kb on + strand at 378.674 kb on + strand at 378.675 kb on - strand at 378.675 kb on - strand at 378.675 kb on - strand at 378.683 kb on + strand at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.117 kb on - strand, within RPSI07_RS01385 at 379.117 kb on - strand, within RPSI07_RS01385 at 379.117 kb on - strand, within RPSI07_RS01385 at 379.117 kb on - strand, within RPSI07_RS01385 at 379.117 kb on - strand, within RPSI07_RS01385 at 379.117 kb on - strand, within RPSI07_RS01385 at 379.119 kb on + strand, within RPSI07_RS01385 at 379.119 kb on + strand, within RPSI07_RS01385 at 379.119 kb on + strand, within RPSI07_RS01385 at 379.119 kb on + strand, within RPSI07_RS01385 at 379.119 kb on + strand, within RPSI07_RS01385 at 379.119 kb on + strand, within RPSI07_RS01385 at 379.119 kb on + strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.196 kb on + strand, within RPSI07_RS01385 at 379.196 kb on + strand, within RPSI07_RS01385 at 379.197 kb on - strand, within RPSI07_RS01385 at 379.197 kb on - strand, within RPSI07_RS01385 at 379.197 kb on - strand, within RPSI07_RS01385 at 379.197 kb on - strand, within RPSI07_RS01385 at 379.197 kb on - strand, within RPSI07_RS01385 at 379.197 kb on - strand, within RPSI07_RS01385 at 379.197 kb on - strand, within RPSI07_RS01385
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 377,394 - +1.5 377,489 + -2.1 377,534 - -2.6 377,534 - -0.5 377,573 - -0.7 377,573 - +0.8 377,615 + -2.1 377,615 + -0.1 377,615 + -0.1 377,616 - -1.2 377,620 - +2.9 377,750 + -0.1 377,750 + -0.7 377,750 + +0.3 377,808 - RPSI07_RS01375 0.13 -0.4 377,808 - RPSI07_RS01375 0.13 +0.1 377,978 + RPSI07_RS01375 0.50 +3.5 377,978 + RPSI07_RS01375 0.50 -1.7 377,978 + RPSI07_RS01375 0.50 -0.4 377,979 - RPSI07_RS01375 0.50 -0.4 377,979 - RPSI07_RS01375 0.50 +1.4 377,992 + RPSI07_RS01375 0.53 +0.9 377,993 + RPSI07_RS01375 0.53 +1.1 377,993 + RPSI07_RS01375 0.53 +0.2 378,009 + RPSI07_RS01375 0.56 -1.7 378,010 - RPSI07_RS01375 0.56 +0.2 378,186 + -1.9 378,291 - +2.5 378,291 - -0.1 378,291 - -1.2 378,674 + +0.5 378,674 + +0.2 378,674 + -2.8 378,674 + +0.1 378,674 + +0.6 378,674 + -0.1 378,675 - +0.9 378,675 - -0.3 378,675 - -0.3 378,683 + +1.2 379,116 + RPSI07_RS01385 0.42 +1.4 379,116 + RPSI07_RS01385 0.42 -0.7 379,116 + RPSI07_RS01385 0.42 +0.0 379,116 + RPSI07_RS01385 0.42 +0.0 379,116 + RPSI07_RS01385 0.42 -0.3 379,116 + RPSI07_RS01385 0.42 -0.7 379,116 + RPSI07_RS01385 0.42 +0.5 379,116 + RPSI07_RS01385 0.42 -0.1 379,116 + RPSI07_RS01385 0.42 -0.0 379,116 + RPSI07_RS01385 0.42 -0.4 379,117 - RPSI07_RS01385 0.42 +0.5 379,117 - RPSI07_RS01385 0.42 -0.7 379,117 - RPSI07_RS01385 0.42 -0.7 379,117 - RPSI07_RS01385 0.42 -0.6 379,117 - RPSI07_RS01385 0.42 +3.2 379,117 - RPSI07_RS01385 0.42 -0.9 379,119 + RPSI07_RS01385 0.42 -0.8 379,119 + RPSI07_RS01385 0.42 +0.7 379,119 + RPSI07_RS01385 0.42 +0.9 379,119 + RPSI07_RS01385 0.42 -0.5 379,119 + RPSI07_RS01385 0.42 -2.8 379,119 + RPSI07_RS01385 0.42 +0.9 379,119 + RPSI07_RS01385 0.42 -1.4 379,120 - RPSI07_RS01385 0.42 -0.5 379,120 - RPSI07_RS01385 0.42 +1.9 379,120 - RPSI07_RS01385 0.42 -0.8 379,120 - RPSI07_RS01385 0.42 -0.9 379,120 - RPSI07_RS01385 0.42 -1.6 379,120 - RPSI07_RS01385 0.42 +0.4 379,120 - RPSI07_RS01385 0.42 +0.6 379,120 - RPSI07_RS01385 0.42 +0.3 379,196 + RPSI07_RS01385 0.51 +1.7 379,196 + RPSI07_RS01385 0.51 +0.8 379,197 - RPSI07_RS01385 0.51 +0.2 379,197 - RPSI07_RS01385 0.51 -0.1 379,197 - RPSI07_RS01385 0.51 +0.7 379,197 - RPSI07_RS01385 0.51 +0.2 379,197 - RPSI07_RS01385 0.51 -0.7 379,197 - RPSI07_RS01385 0.51 +1.2 379,197 - RPSI07_RS01385 0.51 -0.0
Or see this region's nucleotide sequence