Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21765 and RPSI07_RS21770 are separated by 116 nucleotides RPSI07_RS21770 and RPSI07_RS21775 are separated by 51 nucleotides RPSI07_RS21775 and RPSI07_RS21780 are separated by 25 nucleotides
RPSI07_RS21765: RPSI07_RS21765 - membrane protein, at 3,043,513 to 3,044,550
_RS21765
RPSI07_RS21770: RPSI07_RS21770 - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, at 3,044,667 to 3,045,398
_RS21770
RPSI07_RS21775: RPSI07_RS21775 - DUF971 domain-containing protein, at 3,045,450 to 3,045,866
_RS21775
RPSI07_RS21780: RPSI07_RS21780 - ABC transporter ATP-binding protein/permease, at 3,045,892 to 3,047,736
_RS21780
Position (kb)
3045
3046 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3044.521 kb on + strand at 3044.582 kb on + strand at 3044.585 kb on + strand at 3044.586 kb on - strand at 3045.536 kb on - strand, within RPSI07_RS21775 at 3045.581 kb on - strand, within RPSI07_RS21775 at 3045.581 kb on - strand, within RPSI07_RS21775 at 3045.581 kb on - strand, within RPSI07_RS21775 at 3045.581 kb on - strand, within RPSI07_RS21775 at 3045.593 kb on - strand, within RPSI07_RS21775 at 3045.638 kb on - strand, within RPSI07_RS21775 at 3045.752 kb on - strand, within RPSI07_RS21775 at 3045.939 kb on - strand at 3045.944 kb on - strand at 3045.974 kb on + strand at 3045.974 kb on + strand at 3045.975 kb on - strand at 3045.975 kb on - strand at 3045.975 kb on - strand at 3046.068 kb on - strand at 3046.125 kb on - strand, within RPSI07_RS21780 at 3046.125 kb on - strand, within RPSI07_RS21780 at 3046.334 kb on + strand, within RPSI07_RS21780 at 3046.334 kb on + strand, within RPSI07_RS21780 at 3046.335 kb on - strand, within RPSI07_RS21780 at 3046.335 kb on - strand, within RPSI07_RS21780 at 3046.397 kb on + strand, within RPSI07_RS21780 at 3046.398 kb on - strand, within RPSI07_RS21780 at 3046.700 kb on + strand, within RPSI07_RS21780 at 3046.700 kb on + strand, within RPSI07_RS21780 at 3046.701 kb on - strand, within RPSI07_RS21780 at 3046.701 kb on - strand, within RPSI07_RS21780 at 3046.701 kb on - strand, within RPSI07_RS21780 at 3046.793 kb on + strand, within RPSI07_RS21780
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 3,044,521 + -1.7 3,044,582 + -1.3 3,044,585 + -1.6 3,044,586 - +0.6 3,045,536 - RPSI07_RS21775 0.21 +0.3 3,045,581 - RPSI07_RS21775 0.31 -0.5 3,045,581 - RPSI07_RS21775 0.31 -1.4 3,045,581 - RPSI07_RS21775 0.31 +0.1 3,045,581 - RPSI07_RS21775 0.31 +0.7 3,045,593 - RPSI07_RS21775 0.34 +0.4 3,045,638 - RPSI07_RS21775 0.45 +0.8 3,045,752 - RPSI07_RS21775 0.72 +0.6 3,045,939 - -0.5 3,045,944 - +2.8 3,045,974 + -0.2 3,045,974 + +0.5 3,045,975 - +1.3 3,045,975 - +0.0 3,045,975 - -0.1 3,046,068 - -0.3 3,046,125 - RPSI07_RS21780 0.13 +3.0 3,046,125 - RPSI07_RS21780 0.13 +0.9 3,046,334 + RPSI07_RS21780 0.24 -1.8 3,046,334 + RPSI07_RS21780 0.24 -0.7 3,046,335 - RPSI07_RS21780 0.24 +0.4 3,046,335 - RPSI07_RS21780 0.24 +1.5 3,046,397 + RPSI07_RS21780 0.27 +1.2 3,046,398 - RPSI07_RS21780 0.27 +0.1 3,046,700 + RPSI07_RS21780 0.44 +0.0 3,046,700 + RPSI07_RS21780 0.44 +1.2 3,046,701 - RPSI07_RS21780 0.44 +0.4 3,046,701 - RPSI07_RS21780 0.44 +0.3 3,046,701 - RPSI07_RS21780 0.44 +0.8 3,046,793 + RPSI07_RS21780 0.49 +0.6
Or see this region's nucleotide sequence