Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21565 and RPSI07_RS21570 overlap by 1 nucleotides RPSI07_RS21570 and RPSI07_RS21575 are separated by 16 nucleotides RPSI07_RS21575 and RPSI07_RS21580 overlap by 4 nucleotides
RPSI07_RS21565: RPSI07_RS21565 - histidine phosphatase family protein, at 3,000,739 to 3,001,419
_RS21565
RPSI07_RS21570: RPSI07_RS21570 - YbhB/YbcL family Raf kinase inhibitor-like protein, at 3,001,419 to 3,002,054
_RS21570
RPSI07_RS21575: RPSI07_RS21575 - D-tyrosyl-tRNA(Tyr) deacylase, at 3,002,071 to 3,002,571
_RS21575
RPSI07_RS21580: RPSI07_RS21580 - tyrosine--tRNA ligase, at 3,002,568 to 3,003,839
_RS21580
Position (kb)
3002
3003 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3001.103 kb on + strand, within RPSI07_RS21565 at 3001.103 kb on + strand, within RPSI07_RS21565 at 3001.127 kb on + strand, within RPSI07_RS21565 at 3001.226 kb on + strand, within RPSI07_RS21565 at 3001.227 kb on - strand, within RPSI07_RS21565 at 3001.227 kb on - strand, within RPSI07_RS21565 at 3001.227 kb on - strand, within RPSI07_RS21565 at 3001.227 kb on - strand, within RPSI07_RS21565 at 3001.453 kb on + strand at 3001.453 kb on + strand at 3001.454 kb on - strand at 3001.454 kb on - strand at 3001.561 kb on + strand, within RPSI07_RS21570 at 3001.576 kb on + strand, within RPSI07_RS21570 at 3001.576 kb on + strand, within RPSI07_RS21570 at 3001.577 kb on - strand, within RPSI07_RS21570 at 3001.621 kb on + strand, within RPSI07_RS21570 at 3001.621 kb on + strand, within RPSI07_RS21570 at 3001.621 kb on + strand, within RPSI07_RS21570 at 3001.621 kb on + strand, within RPSI07_RS21570 at 3001.622 kb on - strand, within RPSI07_RS21570 at 3001.627 kb on + strand, within RPSI07_RS21570 at 3001.627 kb on + strand, within RPSI07_RS21570 at 3001.627 kb on + strand, within RPSI07_RS21570 at 3001.628 kb on - strand, within RPSI07_RS21570 at 3001.628 kb on - strand, within RPSI07_RS21570 at 3001.628 kb on - strand, within RPSI07_RS21570 at 3001.630 kb on + strand, within RPSI07_RS21570 at 3001.631 kb on - strand, within RPSI07_RS21570 at 3001.672 kb on + strand, within RPSI07_RS21570 at 3002.029 kb on + strand at 3002.240 kb on + strand, within RPSI07_RS21575 at 3002.418 kb on - strand, within RPSI07_RS21575
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 3,001,103 + RPSI07_RS21565 0.53 -0.4 3,001,103 + RPSI07_RS21565 0.53 -0.0 3,001,127 + RPSI07_RS21565 0.57 -0.6 3,001,226 + RPSI07_RS21565 0.72 -0.6 3,001,227 - RPSI07_RS21565 0.72 -0.4 3,001,227 - RPSI07_RS21565 0.72 +2.6 3,001,227 - RPSI07_RS21565 0.72 -0.3 3,001,227 - RPSI07_RS21565 0.72 +0.2 3,001,453 + +0.8 3,001,453 + +0.2 3,001,454 - -0.7 3,001,454 - +0.7 3,001,561 + RPSI07_RS21570 0.22 +1.3 3,001,576 + RPSI07_RS21570 0.25 -0.2 3,001,576 + RPSI07_RS21570 0.25 -0.8 3,001,577 - RPSI07_RS21570 0.25 +0.1 3,001,621 + RPSI07_RS21570 0.32 -2.6 3,001,621 + RPSI07_RS21570 0.32 +2.8 3,001,621 + RPSI07_RS21570 0.32 +2.2 3,001,621 + RPSI07_RS21570 0.32 -0.9 3,001,622 - RPSI07_RS21570 0.32 -0.9 3,001,627 + RPSI07_RS21570 0.33 -0.6 3,001,627 + RPSI07_RS21570 0.33 -0.4 3,001,627 + RPSI07_RS21570 0.33 +0.2 3,001,628 - RPSI07_RS21570 0.33 -0.5 3,001,628 - RPSI07_RS21570 0.33 +0.1 3,001,628 - RPSI07_RS21570 0.33 +1.2 3,001,630 + RPSI07_RS21570 0.33 -0.8 3,001,631 - RPSI07_RS21570 0.33 +2.1 3,001,672 + RPSI07_RS21570 0.40 +1.0 3,002,029 + +0.2 3,002,240 + RPSI07_RS21575 0.34 -1.3 3,002,418 - RPSI07_RS21575 0.69 -0.6
Or see this region's nucleotide sequence