Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS19575 and RPSI07_RS19580 are separated by 122 nucleotides RPSI07_RS19580 and RPSI07_RS19585 are separated by 203 nucleotides RPSI07_RS19585 and RPSI07_RS19590 are separated by 1 nucleotides
RPSI07_RS19575: RPSI07_RS19575 - hypothetical protein, at 2,571,507 to 2,571,911
_RS19575
RPSI07_RS19580: RPSI07_RS19580 - hypothetical protein, at 2,572,034 to 2,572,216
_RS19580
RPSI07_RS19585: RPSI07_RS19585 - NINE protein, at 2,572,420 to 2,572,878
_RS19585
RPSI07_RS19590: RPSI07_RS19590 - radical SAM protein, at 2,572,880 to 2,574,007
_RS19590
Position (kb)
2572
2573 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2571.502 kb on + strand at 2571.502 kb on + strand at 2571.502 kb on + strand at 2571.503 kb on - strand at 2571.503 kb on - strand at 2571.503 kb on - strand at 2571.927 kb on + strand at 2571.928 kb on - strand at 2571.973 kb on - strand at 2571.979 kb on + strand at 2571.980 kb on - strand at 2572.057 kb on + strand, within RPSI07_RS19580 at 2572.057 kb on + strand, within RPSI07_RS19580 at 2572.058 kb on - strand, within RPSI07_RS19580 at 2572.058 kb on - strand, within RPSI07_RS19580 at 2572.058 kb on - strand, within RPSI07_RS19580 at 2572.372 kb on + strand at 2572.372 kb on + strand at 2572.372 kb on + strand at 2572.425 kb on + strand at 2572.425 kb on + strand at 2572.425 kb on + strand at 2572.425 kb on + strand at 2572.426 kb on - strand at 2572.426 kb on - strand at 2572.426 kb on - strand at 2572.426 kb on - strand at 2572.426 kb on - strand at 2572.510 kb on + strand, within RPSI07_RS19585 at 2572.834 kb on + strand at 2572.834 kb on + strand at 2572.834 kb on + strand at 2572.835 kb on - strand at 2572.983 kb on + strand at 2572.983 kb on + strand at 2572.983 kb on + strand at 2572.984 kb on - strand at 2572.984 kb on - strand at 2573.013 kb on + strand, within RPSI07_RS19590 at 2573.014 kb on - strand, within RPSI07_RS19590 at 2573.014 kb on - strand, within RPSI07_RS19590 at 2573.082 kb on + strand, within RPSI07_RS19590 at 2573.083 kb on - strand, within RPSI07_RS19590 at 2573.083 kb on - strand, within RPSI07_RS19590 at 2573.206 kb on - strand, within RPSI07_RS19590 at 2573.339 kb on - strand, within RPSI07_RS19590 at 2573.339 kb on - strand, within RPSI07_RS19590 at 2573.339 kb on - strand, within RPSI07_RS19590 at 2573.339 kb on - strand, within RPSI07_RS19590 at 2573.339 kb on - strand, within RPSI07_RS19590 at 2573.525 kb on + strand, within RPSI07_RS19590 at 2573.712 kb on + strand, within RPSI07_RS19590 at 2573.712 kb on + strand, within RPSI07_RS19590 at 2573.713 kb on - strand, within RPSI07_RS19590 at 2573.739 kb on + strand, within RPSI07_RS19590 at 2573.739 kb on + strand, within RPSI07_RS19590 at 2573.740 kb on - strand, within RPSI07_RS19590 at 2573.740 kb on - strand, within RPSI07_RS19590 at 2573.740 kb on - strand, within RPSI07_RS19590 at 2573.743 kb on + strand, within RPSI07_RS19590 at 2573.743 kb on + strand, within RPSI07_RS19590 at 2573.744 kb on - strand, within RPSI07_RS19590
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 2,571,502 + -1.5 2,571,502 + +2.7 2,571,502 + -1.8 2,571,503 - +0.3 2,571,503 - -0.0 2,571,503 - -0.2 2,571,927 + +0.7 2,571,928 - +0.7 2,571,973 - -1.9 2,571,979 + +1.5 2,571,980 - -2.0 2,572,057 + RPSI07_RS19580 0.13 +1.0 2,572,057 + RPSI07_RS19580 0.13 +0.2 2,572,058 - RPSI07_RS19580 0.13 +1.6 2,572,058 - RPSI07_RS19580 0.13 -2.3 2,572,058 - RPSI07_RS19580 0.13 +0.1 2,572,372 + -0.7 2,572,372 + -0.7 2,572,372 + +0.4 2,572,425 + -2.1 2,572,425 + +0.0 2,572,425 + -0.7 2,572,425 + -0.8 2,572,426 - -0.6 2,572,426 - +0.4 2,572,426 - +1.0 2,572,426 - +1.2 2,572,426 - -0.4 2,572,510 + RPSI07_RS19585 0.20 +1.4 2,572,834 + +1.6 2,572,834 + +0.2 2,572,834 + +1.0 2,572,835 - +0.0 2,572,983 + +1.0 2,572,983 + -0.3 2,572,983 + +2.0 2,572,984 - +1.3 2,572,984 - -0.5 2,573,013 + RPSI07_RS19590 0.12 -2.0 2,573,014 - RPSI07_RS19590 0.12 -0.1 2,573,014 - RPSI07_RS19590 0.12 +0.0 2,573,082 + RPSI07_RS19590 0.18 +1.7 2,573,083 - RPSI07_RS19590 0.18 +0.9 2,573,083 - RPSI07_RS19590 0.18 +0.0 2,573,206 - RPSI07_RS19590 0.29 +3.4 2,573,339 - RPSI07_RS19590 0.41 +0.0 2,573,339 - RPSI07_RS19590 0.41 +0.2 2,573,339 - RPSI07_RS19590 0.41 +1.6 2,573,339 - RPSI07_RS19590 0.41 +2.6 2,573,339 - RPSI07_RS19590 0.41 -1.0 2,573,525 + RPSI07_RS19590 0.57 -0.7 2,573,712 + RPSI07_RS19590 0.74 +1.0 2,573,712 + RPSI07_RS19590 0.74 -0.3 2,573,713 - RPSI07_RS19590 0.74 +0.0 2,573,739 + RPSI07_RS19590 0.76 -0.5 2,573,739 + RPSI07_RS19590 0.76 +2.6 2,573,740 - RPSI07_RS19590 0.76 +0.7 2,573,740 - RPSI07_RS19590 0.76 -0.2 2,573,740 - RPSI07_RS19590 0.76 -0.2 2,573,743 + RPSI07_RS19590 0.77 +1.9 2,573,743 + RPSI07_RS19590 0.77 +0.7 2,573,744 - RPSI07_RS19590 0.77 -1.0
Or see this region's nucleotide sequence