Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS16575 and RPSI07_RS16580 are separated by 776 nucleotides RPSI07_RS16580 and RPSI07_RS16585 are separated by 110 nucleotides RPSI07_RS16585 and RPSI07_RS16590 are separated by 77 nucleotides
RPSI07_RS16575: RPSI07_RS16575 - type III effector protein, at 1,888,686 to 1,896,041
_RS16575
RPSI07_RS16580: RPSI07_RS16580 - integrase, at 1,896,818 to 1,897,770
_RS16580
RPSI07_RS16585: RPSI07_RS16585 - lytic transglycosylase domain-containing protein, at 1,897,881 to 1,898,510
_RS16585
RPSI07_RS16590: RPSI07_RS16590 - cytochrome C, at 1,898,588 to 1,899,790
_RS16590
Position (kb)
1896
1897
1898 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1895.882 kb on - strand at 1895.918 kb on - strand at 1895.918 kb on - strand at 1896.123 kb on + strand at 1896.124 kb on - strand at 1896.140 kb on + strand at 1896.140 kb on + strand at 1896.141 kb on - strand at 1896.141 kb on - strand at 1896.141 kb on - strand at 1896.141 kb on - strand at 1896.389 kb on - strand at 1896.389 kb on - strand at 1896.389 kb on - strand at 1896.392 kb on - strand at 1896.455 kb on + strand at 1896.455 kb on + strand at 1896.456 kb on - strand at 1896.461 kb on + strand at 1896.462 kb on - strand at 1896.901 kb on + strand at 1896.935 kb on + strand, within RPSI07_RS16580 at 1896.990 kb on + strand, within RPSI07_RS16580 at 1896.991 kb on - strand, within RPSI07_RS16580 at 1896.998 kb on + strand, within RPSI07_RS16580 at 1897.010 kb on + strand, within RPSI07_RS16580 at 1897.011 kb on - strand, within RPSI07_RS16580 at 1897.011 kb on - strand, within RPSI07_RS16580 at 1897.020 kb on + strand, within RPSI07_RS16580 at 1897.022 kb on + strand, within RPSI07_RS16580 at 1897.022 kb on + strand, within RPSI07_RS16580 at 1897.022 kb on + strand, within RPSI07_RS16580 at 1897.022 kb on + strand, within RPSI07_RS16580 at 1897.022 kb on + strand, within RPSI07_RS16580 at 1897.022 kb on + strand, within RPSI07_RS16580 at 1897.023 kb on - strand, within RPSI07_RS16580 at 1897.023 kb on - strand, within RPSI07_RS16580 at 1897.023 kb on - strand, within RPSI07_RS16580 at 1897.041 kb on + strand, within RPSI07_RS16580 at 1897.041 kb on + strand, within RPSI07_RS16580 at 1897.042 kb on - strand, within RPSI07_RS16580 at 1897.042 kb on - strand, within RPSI07_RS16580 at 1897.042 kb on - strand, within RPSI07_RS16580 at 1897.047 kb on + strand, within RPSI07_RS16580 at 1897.047 kb on + strand, within RPSI07_RS16580 at 1897.048 kb on - strand, within RPSI07_RS16580 at 1897.048 kb on - strand, within RPSI07_RS16580 at 1897.048 kb on - strand, within RPSI07_RS16580 at 1897.229 kb on + strand, within RPSI07_RS16580 at 1897.229 kb on + strand, within RPSI07_RS16580 at 1897.252 kb on + strand, within RPSI07_RS16580 at 1897.283 kb on - strand, within RPSI07_RS16580 at 1897.323 kb on + strand, within RPSI07_RS16580 at 1897.324 kb on - strand, within RPSI07_RS16580 at 1897.366 kb on - strand, within RPSI07_RS16580 at 1897.529 kb on - strand, within RPSI07_RS16580 at 1897.552 kb on + strand, within RPSI07_RS16580 at 1897.552 kb on + strand, within RPSI07_RS16580 at 1897.552 kb on + strand, within RPSI07_RS16580 at 1897.552 kb on + strand, within RPSI07_RS16580 at 1897.553 kb on - strand, within RPSI07_RS16580 at 1897.553 kb on - strand, within RPSI07_RS16580 at 1897.674 kb on - strand, within RPSI07_RS16580 at 1897.704 kb on + strand at 1897.704 kb on + strand at 1897.704 kb on + strand at 1897.704 kb on + strand at 1897.704 kb on + strand at 1897.708 kb on + strand at 1897.709 kb on - strand at 1897.709 kb on - strand at 1897.709 kb on - strand at 1897.770 kb on + strand at 1897.771 kb on - strand at 1897.771 kb on - strand at 1897.771 kb on - strand at 1897.771 kb on - strand at 1897.822 kb on + strand at 1897.823 kb on + strand at 1897.823 kb on + strand at 1897.824 kb on - strand at 1897.824 kb on - strand at 1897.824 kb on - strand at 1897.824 kb on - strand at 1897.824 kb on - strand at 1897.824 kb on - strand at 1897.837 kb on - strand at 1897.837 kb on - strand at 1897.953 kb on + strand, within RPSI07_RS16585 at 1897.953 kb on + strand, within RPSI07_RS16585 at 1897.953 kb on + strand, within RPSI07_RS16585 at 1897.953 kb on + strand, within RPSI07_RS16585 at 1897.954 kb on - strand, within RPSI07_RS16585 at 1897.954 kb on - strand, within RPSI07_RS16585 at 1897.954 kb on - strand, within RPSI07_RS16585 at 1898.283 kb on - strand, within RPSI07_RS16585 at 1898.335 kb on - strand, within RPSI07_RS16585 at 1898.392 kb on - strand, within RPSI07_RS16585 at 1898.459 kb on + strand at 1898.460 kb on + strand at 1898.460 kb on + strand at 1898.460 kb on + strand at 1898.460 kb on + strand at 1898.461 kb on - strand at 1898.461 kb on - strand at 1898.461 kb on - strand at 1898.461 kb on - strand at 1898.461 kb on - strand at 1898.461 kb on - strand at 1898.461 kb on - strand at 1898.461 kb on - strand at 1898.461 kb on - strand at 1898.508 kb on + strand at 1898.508 kb on + strand at 1898.509 kb on - strand at 1898.559 kb on + strand at 1898.559 kb on + strand at 1898.560 kb on - strand at 1898.560 kb on - strand at 1898.624 kb on + strand at 1898.704 kb on + strand at 1898.704 kb on + strand at 1898.705 kb on - strand at 1898.743 kb on + strand, within RPSI07_RS16590 at 1898.743 kb on + strand, within RPSI07_RS16590 at 1898.743 kb on + strand, within RPSI07_RS16590 at 1898.743 kb on + strand, within RPSI07_RS16590 at 1898.764 kb on + strand, within RPSI07_RS16590 at 1898.765 kb on - strand, within RPSI07_RS16590 at 1898.765 kb on - strand, within RPSI07_RS16590 at 1898.765 kb on - strand, within RPSI07_RS16590 at 1898.765 kb on - strand, within RPSI07_RS16590
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 1,895,882 - +0.2 1,895,918 - -1.6 1,895,918 - +2.6 1,896,123 + -0.5 1,896,124 - -1.1 1,896,140 + +0.3 1,896,140 + +0.3 1,896,141 - -0.3 1,896,141 - -1.4 1,896,141 - -0.9 1,896,141 - -2.5 1,896,389 - +2.0 1,896,389 - -0.6 1,896,389 - +3.2 1,896,392 - -1.0 1,896,455 + -1.0 1,896,455 + -0.6 1,896,456 - +0.7 1,896,461 + -0.7 1,896,462 - +0.7 1,896,901 + +2.3 1,896,935 + RPSI07_RS16580 0.12 +0.0 1,896,990 + RPSI07_RS16580 0.18 +1.0 1,896,991 - RPSI07_RS16580 0.18 -0.9 1,896,998 + RPSI07_RS16580 0.19 +0.6 1,897,010 + RPSI07_RS16580 0.20 -0.7 1,897,011 - RPSI07_RS16580 0.20 -0.1 1,897,011 - RPSI07_RS16580 0.20 +1.7 1,897,020 + RPSI07_RS16580 0.21 -0.1 1,897,022 + RPSI07_RS16580 0.21 -0.8 1,897,022 + RPSI07_RS16580 0.21 +1.5 1,897,022 + RPSI07_RS16580 0.21 +0.0 1,897,022 + RPSI07_RS16580 0.21 -1.9 1,897,022 + RPSI07_RS16580 0.21 +1.8 1,897,022 + RPSI07_RS16580 0.21 -4.0 1,897,023 - RPSI07_RS16580 0.22 +1.4 1,897,023 - RPSI07_RS16580 0.22 +2.6 1,897,023 - RPSI07_RS16580 0.22 -0.7 1,897,041 + RPSI07_RS16580 0.23 +0.2 1,897,041 + RPSI07_RS16580 0.23 +0.4 1,897,042 - RPSI07_RS16580 0.24 +1.1 1,897,042 - RPSI07_RS16580 0.24 +2.0 1,897,042 - RPSI07_RS16580 0.24 -1.2 1,897,047 + RPSI07_RS16580 0.24 -2.0 1,897,047 + RPSI07_RS16580 0.24 +1.1 1,897,048 - RPSI07_RS16580 0.24 +0.5 1,897,048 - RPSI07_RS16580 0.24 +0.5 1,897,048 - RPSI07_RS16580 0.24 -1.0 1,897,229 + RPSI07_RS16580 0.43 +0.3 1,897,229 + RPSI07_RS16580 0.43 +1.0 1,897,252 + RPSI07_RS16580 0.46 -1.6 1,897,283 - RPSI07_RS16580 0.49 +0.2 1,897,323 + RPSI07_RS16580 0.53 +3.1 1,897,324 - RPSI07_RS16580 0.53 +1.0 1,897,366 - RPSI07_RS16580 0.58 +1.0 1,897,529 - RPSI07_RS16580 0.75 +0.7 1,897,552 + RPSI07_RS16580 0.77 -0.7 1,897,552 + RPSI07_RS16580 0.77 -0.2 1,897,552 + RPSI07_RS16580 0.77 +0.2 1,897,552 + RPSI07_RS16580 0.77 -0.4 1,897,553 - RPSI07_RS16580 0.77 +0.6 1,897,553 - RPSI07_RS16580 0.77 -0.4 1,897,674 - RPSI07_RS16580 0.90 -0.4 1,897,704 + +0.7 1,897,704 + +1.2 1,897,704 + -0.2 1,897,704 + -0.6 1,897,704 + -0.2 1,897,708 + -0.1 1,897,709 - +1.0 1,897,709 - -0.1 1,897,709 - -0.6 1,897,770 + +0.7 1,897,771 - -0.8 1,897,771 - +1.3 1,897,771 - -0.5 1,897,771 - -0.1 1,897,822 + -3.9 1,897,823 + +2.0 1,897,823 + +1.2 1,897,824 - -1.6 1,897,824 - +0.8 1,897,824 - -0.8 1,897,824 - +1.2 1,897,824 - -0.2 1,897,824 - -0.4 1,897,837 - -0.8 1,897,837 - +1.0 1,897,953 + RPSI07_RS16585 0.11 +1.8 1,897,953 + RPSI07_RS16585 0.11 -0.4 1,897,953 + RPSI07_RS16585 0.11 +2.0 1,897,953 + RPSI07_RS16585 0.11 +0.1 1,897,954 - RPSI07_RS16585 0.12 +0.0 1,897,954 - RPSI07_RS16585 0.12 -0.3 1,897,954 - RPSI07_RS16585 0.12 -0.5 1,898,283 - RPSI07_RS16585 0.64 -1.1 1,898,335 - RPSI07_RS16585 0.72 +3.3 1,898,392 - RPSI07_RS16585 0.81 +0.6 1,898,459 + +0.3 1,898,460 + +1.2 1,898,460 + -0.7 1,898,460 + +0.6 1,898,460 + -0.3 1,898,461 - -1.4 1,898,461 - +0.2 1,898,461 - +1.0 1,898,461 - -0.2 1,898,461 - +2.0 1,898,461 - +0.1 1,898,461 - +1.7 1,898,461 - +0.1 1,898,461 - +1.5 1,898,508 + +3.2 1,898,508 + +0.2 1,898,509 - -1.0 1,898,559 + +1.0 1,898,559 + -0.3 1,898,560 - +0.0 1,898,560 - +0.6 1,898,624 + +4.0 1,898,704 + -0.6 1,898,704 + +3.7 1,898,705 - +0.1 1,898,743 + RPSI07_RS16590 0.13 +1.0 1,898,743 + RPSI07_RS16590 0.13 -2.1 1,898,743 + RPSI07_RS16590 0.13 -0.9 1,898,743 + RPSI07_RS16590 0.13 -1.4 1,898,764 + RPSI07_RS16590 0.15 -1.8 1,898,765 - RPSI07_RS16590 0.15 +1.3 1,898,765 - RPSI07_RS16590 0.15 +1.2 1,898,765 - RPSI07_RS16590 0.15 +1.0 1,898,765 - RPSI07_RS16590 0.15 -0.8
Or see this region's nucleotide sequence