Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS14745

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS14735 and RPSI07_RS14740 are separated by 14 nucleotidesRPSI07_RS14740 and RPSI07_RS14745 are separated by 37 nucleotidesRPSI07_RS14745 and RPSI07_RS14750 are separated by 81 nucleotides RPSI07_RS14735: RPSI07_RS14735 - site-specific DNA-methyltransferase, at 1,481,864 to 1,482,676 _RS14735 RPSI07_RS14740: RPSI07_RS14740 - tryptophan synthase subunit alpha, at 1,482,691 to 1,483,488 _RS14740 RPSI07_RS14745: RPSI07_RS14745 - acetyl-CoA carboxylase carboxyltransferase subunit beta, at 1,483,526 to 1,484,398 _RS14745 RPSI07_RS14750: RPSI07_RS14750 - bifunctional tetrahydrofolate synthase/dihydrofolate synthase, at 1,484,480 to 1,485,778 _RS14750 Position (kb) 1483 1484 1485Strain fitness (log2 ratio) -1 0 1 2at 1482.690 kb on + strandat 1482.690 kb on + strandat 1483.207 kb on + strand, within RPSI07_RS14740at 1483.216 kb on + strand, within RPSI07_RS14740at 1484.470 kb on + strandat 1484.470 kb on + strandat 1484.474 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
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1,482,690 + +2.4
1,482,690 + -0.5
1,483,207 + RPSI07_RS14740 0.65 +0.0
1,483,216 + RPSI07_RS14740 0.66 -0.5
1,484,470 + +0.6
1,484,470 + -1.0
1,484,474 + +2.0

Or see this region's nucleotide sequence