Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS14720 and RPSI07_RS14725 overlap by 4 nucleotides RPSI07_RS14725 and RPSI07_RS14730 are separated by 94 nucleotides RPSI07_RS14730 and RPSI07_RS14735 are separated by 4 nucleotides RPSI07_RS14735 and RPSI07_RS14740 are separated by 14 nucleotides
RPSI07_RS14720: RPSI07_RS14720 - tRNA pseudouridine(38-40) synthase TruA, at 1,479,046 to 1,479,870
_RS14720
RPSI07_RS14725: RPSI07_RS14725 - phosphoribosylanthranilate isomerase, at 1,479,867 to 1,480,571
_RS14725
RPSI07_RS14730: RPSI07_RS14730 - tryptophan synthase subunit beta, at 1,480,666 to 1,481,859
_RS14730
RPSI07_RS14735: RPSI07_RS14735 - site-specific DNA-methyltransferase, at 1,481,864 to 1,482,676
_RS14735
RPSI07_RS14740: RPSI07_RS14740 - tryptophan synthase subunit alpha, at 1,482,691 to 1,483,488
_RS14740
Position (kb) 1480
1481
1482 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1479.714 kb on - strand, within RPSI07_RS14720 at 1480.670 kb on - strand at 1480.804 kb on + strand, within RPSI07_RS14730 at 1480.805 kb on - strand, within RPSI07_RS14730 at 1480.850 kb on - strand, within RPSI07_RS14730 at 1480.894 kb on + strand, within RPSI07_RS14730 at 1480.894 kb on + strand, within RPSI07_RS14730 at 1480.895 kb on - strand, within RPSI07_RS14730 at 1481.072 kb on - strand, within RPSI07_RS14730 at 1481.399 kb on - strand, within RPSI07_RS14730 at 1481.404 kb on + strand, within RPSI07_RS14730 at 1481.645 kb on - strand, within RPSI07_RS14730 at 1481.984 kb on + strand, within RPSI07_RS14735 at 1481.984 kb on + strand, within RPSI07_RS14735 at 1482.021 kb on - strand, within RPSI07_RS14735 at 1482.021 kb on - strand, within RPSI07_RS14735 at 1482.086 kb on + strand, within RPSI07_RS14735 at 1482.257 kb on + strand, within RPSI07_RS14735 at 1482.257 kb on + strand, within RPSI07_RS14735 at 1482.690 kb on + strand at 1482.690 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 1,479,714 - RPSI07_RS14720 0.81 -1.3 1,480,670 - +0.0 1,480,804 + RPSI07_RS14730 0.12 +0.0 1,480,805 - RPSI07_RS14730 0.12 +1.0 1,480,850 - RPSI07_RS14730 0.15 +1.0 1,480,894 + RPSI07_RS14730 0.19 +0.0 1,480,894 + RPSI07_RS14730 0.19 +1.0 1,480,895 - RPSI07_RS14730 0.19 -0.6 1,481,072 - RPSI07_RS14730 0.34 +1.0 1,481,399 - RPSI07_RS14730 0.61 +2.0 1,481,404 + RPSI07_RS14730 0.62 +1.0 1,481,645 - RPSI07_RS14730 0.82 -1.3 1,481,984 + RPSI07_RS14735 0.15 -2.6 1,481,984 + RPSI07_RS14735 0.15 -1.9 1,482,021 - RPSI07_RS14735 0.19 -1.0 1,482,021 - RPSI07_RS14735 0.19 +1.0 1,482,086 + RPSI07_RS14735 0.27 +0.5 1,482,257 + RPSI07_RS14735 0.48 -0.1 1,482,257 + RPSI07_RS14735 0.48 -3.3 1,482,690 + +2.4 1,482,690 + -0.5
Or see this region's nucleotide sequence