Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS13075

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS13065 and RPSI07_RS13070 are separated by 701 nucleotidesRPSI07_RS13070 and RPSI07_RS13075 are separated by 3 nucleotidesRPSI07_RS13075 and RPSI07_RS13080 are separated by 110 nucleotides RPSI07_RS13065: RPSI07_RS13065 - DUF2130 domain-containing protein, at 1,117,724 to 1,119,133 _RS13065 RPSI07_RS13070: RPSI07_RS13070 - hypothetical protein, at 1,119,835 to 1,120,110 _RS13070 RPSI07_RS13075: RPSI07_RS13075 - hypothetical protein, at 1,120,114 to 1,120,380 _RS13075 RPSI07_RS13080: RPSI07_RS13080 - argininosuccinate lyase, at 1,120,491 to 1,121,906 _RS13080 Position (kb) 1120 1121Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1119.182 kb on + strandat 1119.183 kb on - strandat 1119.237 kb on - strandat 1119.238 kb on + strandat 1119.238 kb on + strandat 1119.238 kb on + strandat 1119.239 kb on - strandat 1119.239 kb on - strandat 1119.239 kb on - strandat 1119.239 kb on - strandat 1119.370 kb on + strandat 1119.469 kb on + strandat 1119.487 kb on - strandat 1119.487 kb on - strandat 1119.543 kb on + strandat 1119.543 kb on + strandat 1119.544 kb on - strandat 1119.549 kb on + strandat 1119.549 kb on + strandat 1119.643 kb on + strandat 1119.643 kb on + strandat 1119.643 kb on + strandat 1119.644 kb on - strandat 1119.747 kb on + strandat 1119.748 kb on - strandat 1119.748 kb on - strandat 1119.748 kb on - strandat 1119.856 kb on + strandat 1119.856 kb on + strandat 1119.893 kb on + strand, within RPSI07_RS13070at 1119.894 kb on - strand, within RPSI07_RS13070at 1119.988 kb on + strand, within RPSI07_RS13070at 1119.988 kb on + strand, within RPSI07_RS13070at 1119.988 kb on + strand, within RPSI07_RS13070at 1119.988 kb on + strand, within RPSI07_RS13070at 1119.988 kb on + strand, within RPSI07_RS13070at 1119.988 kb on + strand, within RPSI07_RS13070at 1119.988 kb on + strand, within RPSI07_RS13070at 1119.988 kb on + strand, within RPSI07_RS13070at 1119.988 kb on + strand, within RPSI07_RS13070at 1120.021 kb on + strand, within RPSI07_RS13070at 1120.021 kb on + strand, within RPSI07_RS13070at 1120.021 kb on + strand, within RPSI07_RS13070at 1120.022 kb on - strand, within RPSI07_RS13070at 1120.022 kb on - strand, within RPSI07_RS13070at 1120.022 kb on - strand, within RPSI07_RS13070at 1120.022 kb on - strand, within RPSI07_RS13070at 1120.022 kb on - strand, within RPSI07_RS13070at 1120.032 kb on + strand, within RPSI07_RS13070at 1120.175 kb on - strand, within RPSI07_RS13075at 1120.224 kb on + strand, within RPSI07_RS13075at 1120.224 kb on + strand, within RPSI07_RS13075at 1120.224 kb on + strand, within RPSI07_RS13075at 1120.224 kb on + strand, within RPSI07_RS13075at 1120.307 kb on - strand, within RPSI07_RS13075at 1120.339 kb on + strand, within RPSI07_RS13075at 1120.339 kb on + strand, within RPSI07_RS13075at 1120.339 kb on + strand, within RPSI07_RS13075at 1120.339 kb on + strand, within RPSI07_RS13075at 1120.339 kb on + strand, within RPSI07_RS13075at 1120.339 kb on + strand, within RPSI07_RS13075at 1120.339 kb on + strand, within RPSI07_RS13075at 1120.339 kb on + strand, within RPSI07_RS13075at 1120.340 kb on - strand, within RPSI07_RS13075at 1120.340 kb on - strand, within RPSI07_RS13075at 1120.340 kb on - strand, within RPSI07_RS13075at 1120.371 kb on + strandat 1120.371 kb on + strandat 1120.372 kb on - strandat 1120.422 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
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1,119,182 + +0.2
1,119,183 - -0.5
1,119,237 - -0.0
1,119,238 + +0.0
1,119,238 + +0.8
1,119,238 + -0.3
1,119,239 - -0.2
1,119,239 - -1.1
1,119,239 - +1.5
1,119,239 - -0.2
1,119,370 + -0.4
1,119,469 + -0.4
1,119,487 - +0.4
1,119,487 - +1.1
1,119,543 + +1.1
1,119,543 + +0.7
1,119,544 - +0.2
1,119,549 + -0.4
1,119,549 + +1.1
1,119,643 + +1.8
1,119,643 + -2.2
1,119,643 + -0.6
1,119,644 - -0.6
1,119,747 + +0.3
1,119,748 - -1.0
1,119,748 - +0.7
1,119,748 - -0.6
1,119,856 + +2.1
1,119,856 + -0.0
1,119,893 + RPSI07_RS13070 0.21 +0.6
1,119,894 - RPSI07_RS13070 0.21 +1.1
1,119,988 + RPSI07_RS13070 0.55 -0.5
1,119,988 + RPSI07_RS13070 0.55 +0.3
1,119,988 + RPSI07_RS13070 0.55 +1.5
1,119,988 + RPSI07_RS13070 0.55 -0.4
1,119,988 + RPSI07_RS13070 0.55 +0.6
1,119,988 + RPSI07_RS13070 0.55 +0.9
1,119,988 + RPSI07_RS13070 0.55 -0.1
1,119,988 + RPSI07_RS13070 0.55 +0.5
1,119,988 + RPSI07_RS13070 0.55 -0.7
1,120,021 + RPSI07_RS13070 0.67 -0.8
1,120,021 + RPSI07_RS13070 0.67 +2.1
1,120,021 + RPSI07_RS13070 0.67 -0.9
1,120,022 - RPSI07_RS13070 0.68 +0.3
1,120,022 - RPSI07_RS13070 0.68 +1.1
1,120,022 - RPSI07_RS13070 0.68 +0.7
1,120,022 - RPSI07_RS13070 0.68 -0.9
1,120,022 - RPSI07_RS13070 0.68 +0.1
1,120,032 + RPSI07_RS13070 0.71 +0.3
1,120,175 - RPSI07_RS13075 0.23 -0.4
1,120,224 + RPSI07_RS13075 0.41 +0.1
1,120,224 + RPSI07_RS13075 0.41 +1.6
1,120,224 + RPSI07_RS13075 0.41 -0.4
1,120,224 + RPSI07_RS13075 0.41 -0.5
1,120,307 - RPSI07_RS13075 0.72 -0.3
1,120,339 + RPSI07_RS13075 0.84 +0.1
1,120,339 + RPSI07_RS13075 0.84 -1.3
1,120,339 + RPSI07_RS13075 0.84 +2.7
1,120,339 + RPSI07_RS13075 0.84 +0.4
1,120,339 + RPSI07_RS13075 0.84 +3.4
1,120,339 + RPSI07_RS13075 0.84 -0.4
1,120,339 + RPSI07_RS13075 0.84 +0.1
1,120,339 + RPSI07_RS13075 0.84 +1.6
1,120,340 - RPSI07_RS13075 0.85 +4.4
1,120,340 - RPSI07_RS13075 0.85 +0.6
1,120,340 - RPSI07_RS13075 0.85 -0.6
1,120,371 + -0.2
1,120,371 + +0.7
1,120,372 - -1.1
1,120,422 + +0.5

Or see this region's nucleotide sequence