Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS12410 and RPSI07_RS12415 overlap by 4 nucleotides RPSI07_RS12415 and RPSI07_RS12420 are separated by 190 nucleotides
RPSI07_RS12410: RPSI07_RS12410 - cytochrome B6, at 977,415 to 978,764
_RS12410
RPSI07_RS12415: RPSI07_RS12415 - ser/threonine protein phosphatase, at 978,761 to 980,272
_RS12415
RPSI07_RS12420: RPSI07_RS12420 - hypothetical protein, at 980,463 to 980,666
_RS12420
Position (kb) 978
979
980
981 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 977.813 kb on - strand, within RPSI07_RS12410 at 977.813 kb on - strand, within RPSI07_RS12410 at 978.133 kb on + strand, within RPSI07_RS12410 at 978.133 kb on + strand at 978.134 kb on - strand, within RPSI07_RS12410 at 978.391 kb on + strand, within RPSI07_RS12410 at 978.392 kb on - strand, within RPSI07_RS12410 at 978.392 kb on - strand, within RPSI07_RS12410 at 978.392 kb on - strand, within RPSI07_RS12410 at 978.407 kb on - strand, within RPSI07_RS12410 at 978.416 kb on - strand, within RPSI07_RS12410 at 978.416 kb on - strand, within RPSI07_RS12410 at 978.416 kb on - strand, within RPSI07_RS12410 at 978.416 kb on - strand, within RPSI07_RS12410 at 978.416 kb on - strand, within RPSI07_RS12410 at 978.846 kb on + strand at 978.846 kb on + strand at 978.846 kb on + strand at 978.847 kb on - strand at 979.102 kb on - strand, within RPSI07_RS12415 at 979.104 kb on + strand, within RPSI07_RS12415 at 979.105 kb on - strand, within RPSI07_RS12415 at 979.105 kb on - strand, within RPSI07_RS12415 at 979.272 kb on + strand, within RPSI07_RS12415 at 979.272 kb on + strand, within RPSI07_RS12415 at 979.273 kb on - strand, within RPSI07_RS12415 at 979.273 kb on - strand, within RPSI07_RS12415 at 979.372 kb on - strand, within RPSI07_RS12415 at 979.372 kb on - strand, within RPSI07_RS12415 at 979.567 kb on + strand, within RPSI07_RS12415 at 979.568 kb on - strand, within RPSI07_RS12415 at 979.568 kb on - strand, within RPSI07_RS12415 at 979.578 kb on + strand, within RPSI07_RS12415 at 979.636 kb on - strand, within RPSI07_RS12415 at 979.641 kb on + strand, within RPSI07_RS12415 at 979.641 kb on + strand, within RPSI07_RS12415 at 979.641 kb on + strand, within RPSI07_RS12415 at 979.741 kb on + strand, within RPSI07_RS12415 at 979.741 kb on + strand, within RPSI07_RS12415 at 979.742 kb on - strand, within RPSI07_RS12415 at 980.035 kb on + strand, within RPSI07_RS12415 at 980.036 kb on - strand, within RPSI07_RS12415 at 980.036 kb on - strand, within RPSI07_RS12415 at 980.037 kb on + strand, within RPSI07_RS12415 at 980.037 kb on + strand, within RPSI07_RS12415 at 980.037 kb on + strand, within RPSI07_RS12415 at 980.038 kb on - strand, within RPSI07_RS12415 at 980.038 kb on - strand, within RPSI07_RS12415 at 980.038 kb on - strand, within RPSI07_RS12415 at 980.234 kb on + strand at 980.234 kb on + strand at 980.234 kb on + strand at 980.234 kb on + strand at 980.234 kb on + strand at 980.235 kb on - strand at 980.235 kb on - strand at 980.235 kb on - strand at 980.235 kb on - strand at 980.235 kb on - strand at 980.235 kb on - strand at 980.235 kb on - strand at 980.235 kb on - strand at 980.235 kb on - strand at 980.335 kb on - strand at 980.406 kb on + strand at 980.406 kb on + strand at 980.406 kb on + strand at 980.406 kb on + strand at 980.406 kb on + strand at 980.406 kb on + strand at 980.407 kb on - strand at 980.407 kb on - strand at 980.407 kb on - strand at 980.407 kb on - strand at 980.464 kb on + strand at 980.465 kb on - strand at 980.669 kb on + strand at 981.116 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 977,813 - RPSI07_RS12410 0.29 +0.2 977,813 - RPSI07_RS12410 0.29 +2.3 978,133 + RPSI07_RS12410 0.53 +1.3 978,133 + -0.4 978,134 - RPSI07_RS12410 0.53 +1.2 978,391 + RPSI07_RS12410 0.72 +2.2 978,392 - RPSI07_RS12410 0.72 -0.6 978,392 - RPSI07_RS12410 0.72 +0.3 978,392 - RPSI07_RS12410 0.72 -0.8 978,407 - RPSI07_RS12410 0.73 +0.2 978,416 - RPSI07_RS12410 0.74 -2.2 978,416 - RPSI07_RS12410 0.74 -2.0 978,416 - RPSI07_RS12410 0.74 +0.7 978,416 - RPSI07_RS12410 0.74 -0.0 978,416 - RPSI07_RS12410 0.74 -0.8 978,846 + +0.6 978,846 + -0.2 978,846 + -0.2 978,847 - +0.1 979,102 - RPSI07_RS12415 0.23 -0.8 979,104 + RPSI07_RS12415 0.23 +0.9 979,105 - RPSI07_RS12415 0.23 +0.5 979,105 - RPSI07_RS12415 0.23 -0.4 979,272 + RPSI07_RS12415 0.34 -0.0 979,272 + RPSI07_RS12415 0.34 +1.6 979,273 - RPSI07_RS12415 0.34 -2.0 979,273 - RPSI07_RS12415 0.34 +1.2 979,372 - RPSI07_RS12415 0.40 -0.3 979,372 - RPSI07_RS12415 0.40 -2.3 979,567 + RPSI07_RS12415 0.53 -0.9 979,568 - RPSI07_RS12415 0.53 -1.7 979,568 - RPSI07_RS12415 0.53 -0.4 979,578 + RPSI07_RS12415 0.54 -0.8 979,636 - RPSI07_RS12415 0.58 -0.3 979,641 + RPSI07_RS12415 0.58 +0.3 979,641 + RPSI07_RS12415 0.58 +1.2 979,641 + RPSI07_RS12415 0.58 +0.9 979,741 + RPSI07_RS12415 0.65 -0.6 979,741 + RPSI07_RS12415 0.65 -0.7 979,742 - RPSI07_RS12415 0.65 -0.0 980,035 + RPSI07_RS12415 0.84 -1.5 980,036 - RPSI07_RS12415 0.84 -2.2 980,036 - RPSI07_RS12415 0.84 +1.6 980,037 + RPSI07_RS12415 0.84 -1.3 980,037 + RPSI07_RS12415 0.84 +0.6 980,037 + RPSI07_RS12415 0.84 -0.4 980,038 - RPSI07_RS12415 0.84 -1.3 980,038 - RPSI07_RS12415 0.84 -0.3 980,038 - RPSI07_RS12415 0.84 +1.4 980,234 + +1.5 980,234 + +0.2 980,234 + -0.7 980,234 + +1.0 980,234 + -1.9 980,235 - -0.0 980,235 - +1.2 980,235 - -0.2 980,235 - -0.4 980,235 - +0.0 980,235 - +2.7 980,235 - +0.2 980,235 - +3.5 980,235 - +0.1 980,335 - -0.1 980,406 + -1.6 980,406 + +1.7 980,406 + -0.2 980,406 + +0.4 980,406 + +0.1 980,406 + -0.4 980,407 - +2.5 980,407 - +1.9 980,407 - +1.8 980,407 - +0.7 980,464 + -0.1 980,465 - -0.8 980,669 + +1.2 981,116 + +1.9
Or see this region's nucleotide sequence