Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS07675 and RPSI07_RS07680 are separated by 108 nucleotides RPSI07_RS07680 and RPSI07_RS07685 overlap by 4 nucleotides RPSI07_RS07685 and RPSI07_RS07690 are separated by 13 nucleotides RPSI07_RS07690 and RPSI07_RS24825 are separated by 386 nucleotides RPSI07_RS24825 and RPSI07_RS07695 are separated by 273 nucleotides
RPSI07_RS07675: RPSI07_RS07675 - MarR family transcriptional regulator, at 2,042,267 to 2,042,689
_RS07675
RPSI07_RS07680: RPSI07_RS07680 - hypothetical protein, at 2,042,798 to 2,043,106
_RS07680
RPSI07_RS07685: RPSI07_RS07685 - hypothetical protein, at 2,043,103 to 2,043,369
_RS07685
RPSI07_RS07690: RPSI07_RS07690 - PepSY domain-containing protein, at 2,043,383 to 2,045,005
_RS07690
RPSI07_RS24825: RPSI07_RS24825 - hypothetical protein, at 2,045,392 to 2,045,619
_RS24825
RPSI07_RS07695: RPSI07_RS07695 - MarR family transcriptional regulator, at 2,045,893 to 2,046,309
_RS07695
Position (kb)
2043
2044
2045
2046 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 2042.614 kb on - strand, within RPSI07_RS07675 at 2042.614 kb on - strand, within RPSI07_RS07675 at 2042.614 kb on - strand, within RPSI07_RS07675 at 2042.628 kb on + strand, within RPSI07_RS07675 at 2042.628 kb on + strand, within RPSI07_RS07675 at 2042.629 kb on - strand, within RPSI07_RS07675 at 2042.846 kb on + strand, within RPSI07_RS07680 at 2042.846 kb on + strand, within RPSI07_RS07680 at 2042.847 kb on - strand, within RPSI07_RS07680 at 2042.847 kb on - strand, within RPSI07_RS07680 at 2042.847 kb on - strand, within RPSI07_RS07680 at 2042.847 kb on - strand, within RPSI07_RS07680 at 2043.400 kb on - strand at 2043.407 kb on + strand at 2043.407 kb on + strand at 2043.407 kb on + strand at 2043.421 kb on + strand at 2043.421 kb on + strand at 2043.421 kb on + strand at 2043.421 kb on + strand at 2043.422 kb on - strand at 2043.422 kb on - strand at 2043.486 kb on + strand at 2043.486 kb on + strand at 2043.486 kb on + strand at 2043.487 kb on - strand at 2043.623 kb on + strand, within RPSI07_RS07690 at 2043.623 kb on + strand, within RPSI07_RS07690 at 2043.623 kb on + strand, within RPSI07_RS07690 at 2043.623 kb on + strand, within RPSI07_RS07690 at 2043.623 kb on + strand, within RPSI07_RS07690 at 2043.623 kb on + strand, within RPSI07_RS07690 at 2043.623 kb on + strand, within RPSI07_RS07690 at 2043.624 kb on - strand, within RPSI07_RS07690 at 2043.624 kb on - strand, within RPSI07_RS07690 at 2043.624 kb on - strand, within RPSI07_RS07690 at 2043.666 kb on - strand, within RPSI07_RS07690 at 2043.794 kb on + strand, within RPSI07_RS07690 at 2043.795 kb on - strand, within RPSI07_RS07690 at 2043.795 kb on - strand, within RPSI07_RS07690 at 2043.824 kb on + strand, within RPSI07_RS07690 at 2043.824 kb on + strand, within RPSI07_RS07690 at 2043.824 kb on + strand, within RPSI07_RS07690 at 2043.824 kb on + strand, within RPSI07_RS07690 at 2043.824 kb on + strand, within RPSI07_RS07690 at 2043.825 kb on - strand, within RPSI07_RS07690 at 2043.825 kb on - strand, within RPSI07_RS07690 at 2043.825 kb on - strand, within RPSI07_RS07690 at 2043.851 kb on + strand, within RPSI07_RS07690 at 2043.852 kb on - strand, within RPSI07_RS07690 at 2043.852 kb on - strand, within RPSI07_RS07690 at 2044.050 kb on + strand, within RPSI07_RS07690 at 2044.050 kb on + strand, within RPSI07_RS07690 at 2044.050 kb on + strand, within RPSI07_RS07690 at 2044.051 kb on - strand, within RPSI07_RS07690 at 2044.051 kb on - strand, within RPSI07_RS07690 at 2044.292 kb on + strand, within RPSI07_RS07690 at 2044.292 kb on + strand, within RPSI07_RS07690 at 2044.293 kb on - strand, within RPSI07_RS07690 at 2044.391 kb on + strand, within RPSI07_RS07690 at 2044.391 kb on + strand, within RPSI07_RS07690 at 2044.392 kb on - strand, within RPSI07_RS07690 at 2044.392 kb on - strand, within RPSI07_RS07690 at 2044.392 kb on - strand, within RPSI07_RS07690 at 2044.403 kb on + strand, within RPSI07_RS07690 at 2044.404 kb on - strand, within RPSI07_RS07690 at 2044.405 kb on - strand, within RPSI07_RS07690 at 2044.424 kb on + strand, within RPSI07_RS07690 at 2044.424 kb on + strand, within RPSI07_RS07690 at 2044.452 kb on - strand, within RPSI07_RS07690 at 2044.487 kb on + strand, within RPSI07_RS07690 at 2044.487 kb on + strand, within RPSI07_RS07690 at 2044.664 kb on + strand, within RPSI07_RS07690 at 2044.664 kb on + strand, within RPSI07_RS07690 at 2044.664 kb on + strand, within RPSI07_RS07690 at 2044.665 kb on - strand, within RPSI07_RS07690 at 2044.665 kb on - strand, within RPSI07_RS07690 at 2044.665 kb on - strand, within RPSI07_RS07690 at 2044.665 kb on - strand, within RPSI07_RS07690 at 2044.668 kb on - strand, within RPSI07_RS07690 at 2044.880 kb on - strand at 2045.210 kb on + strand at 2045.210 kb on + strand at 2045.226 kb on - strand at 2045.235 kb on - strand at 2045.235 kb on - strand at 2045.264 kb on + strand at 2045.265 kb on - strand at 2045.297 kb on + strand at 2045.297 kb on + strand at 2045.297 kb on + strand at 2045.297 kb on + strand at 2045.298 kb on - strand at 2045.312 kb on + strand at 2045.332 kb on + strand at 2045.332 kb on + strand at 2045.332 kb on + strand at 2045.332 kb on + strand at 2045.333 kb on - strand at 2045.333 kb on - strand at 2045.333 kb on - strand at 2045.333 kb on - strand at 2045.333 kb on - strand at 2045.375 kb on - strand at 2045.390 kb on + strand at 2045.390 kb on + strand at 2045.573 kb on + strand, within RPSI07_RS24825 at 2045.573 kb on + strand, within RPSI07_RS24825 at 2045.573 kb on + strand, within RPSI07_RS24825 at 2045.574 kb on - strand, within RPSI07_RS24825 at 2045.624 kb on + strand at 2045.702 kb on + strand at 2045.740 kb on - strand at 2045.782 kb on + strand at 2045.782 kb on + strand at 2045.782 kb on + strand at 2045.782 kb on + strand at 2045.782 kb on + strand at 2045.783 kb on - strand at 2045.783 kb on - strand at 2045.783 kb on - strand at 2045.783 kb on - strand at 2045.783 kb on - strand at 2045.783 kb on - strand at 2045.783 kb on - strand at 2045.783 kb on - strand at 2045.787 kb on + strand at 2045.787 kb on + strand at 2045.788 kb on - strand at 2045.788 kb on - strand at 2045.788 kb on - strand at 2045.872 kb on - strand at 2045.881 kb on + strand at 2045.882 kb on - strand at 2045.911 kb on + strand at 2045.912 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 2,042,614 - RPSI07_RS07675 0.82 +1.9 2,042,614 - RPSI07_RS07675 0.82 -0.4 2,042,614 - RPSI07_RS07675 0.82 -0.6 2,042,628 + RPSI07_RS07675 0.85 +1.7 2,042,628 + RPSI07_RS07675 0.85 +2.4 2,042,629 - RPSI07_RS07675 0.86 -1.3 2,042,846 + RPSI07_RS07680 0.16 -0.1 2,042,846 + RPSI07_RS07680 0.16 -2.0 2,042,847 - RPSI07_RS07680 0.16 +0.1 2,042,847 - RPSI07_RS07680 0.16 -0.1 2,042,847 - RPSI07_RS07680 0.16 -1.4 2,042,847 - RPSI07_RS07680 0.16 -1.4 2,043,400 - +2.4 2,043,407 + +3.2 2,043,407 + +0.1 2,043,407 + +0.6 2,043,421 + -0.3 2,043,421 + -0.5 2,043,421 + +0.3 2,043,421 + +0.2 2,043,422 - +1.5 2,043,422 - -0.3 2,043,486 + -0.0 2,043,486 + +2.4 2,043,486 + +0.9 2,043,487 - +2.9 2,043,623 + RPSI07_RS07690 0.15 +0.3 2,043,623 + RPSI07_RS07690 0.15 -0.1 2,043,623 + RPSI07_RS07690 0.15 -1.3 2,043,623 + RPSI07_RS07690 0.15 -1.1 2,043,623 + RPSI07_RS07690 0.15 +0.1 2,043,623 + RPSI07_RS07690 0.15 +0.4 2,043,623 + RPSI07_RS07690 0.15 -0.3 2,043,624 - RPSI07_RS07690 0.15 +0.2 2,043,624 - RPSI07_RS07690 0.15 -0.9 2,043,624 - RPSI07_RS07690 0.15 -1.8 2,043,666 - RPSI07_RS07690 0.17 +0.0 2,043,794 + RPSI07_RS07690 0.25 +0.6 2,043,795 - RPSI07_RS07690 0.25 -1.2 2,043,795 - RPSI07_RS07690 0.25 +1.4 2,043,824 + RPSI07_RS07690 0.27 +0.9 2,043,824 + RPSI07_RS07690 0.27 +0.4 2,043,824 + RPSI07_RS07690 0.27 +6.4 2,043,824 + RPSI07_RS07690 0.27 -0.7 2,043,824 + RPSI07_RS07690 0.27 -0.1 2,043,825 - RPSI07_RS07690 0.27 +0.1 2,043,825 - RPSI07_RS07690 0.27 -0.9 2,043,825 - RPSI07_RS07690 0.27 +0.7 2,043,851 + RPSI07_RS07690 0.29 -0.7 2,043,852 - RPSI07_RS07690 0.29 +0.4 2,043,852 - RPSI07_RS07690 0.29 +0.6 2,044,050 + RPSI07_RS07690 0.41 -0.5 2,044,050 + RPSI07_RS07690 0.41 -0.8 2,044,050 + RPSI07_RS07690 0.41 -0.0 2,044,051 - RPSI07_RS07690 0.41 -0.7 2,044,051 - RPSI07_RS07690 0.41 +1.9 2,044,292 + RPSI07_RS07690 0.56 +2.9 2,044,292 + RPSI07_RS07690 0.56 +0.2 2,044,293 - RPSI07_RS07690 0.56 -0.1 2,044,391 + RPSI07_RS07690 0.62 +2.6 2,044,391 + RPSI07_RS07690 0.62 +0.7 2,044,392 - RPSI07_RS07690 0.62 +0.9 2,044,392 - RPSI07_RS07690 0.62 -0.8 2,044,392 - RPSI07_RS07690 0.62 +2.4 2,044,403 + RPSI07_RS07690 0.63 +0.3 2,044,404 - RPSI07_RS07690 0.63 +1.1 2,044,405 - RPSI07_RS07690 0.63 +2.1 2,044,424 + RPSI07_RS07690 0.64 +0.4 2,044,424 + RPSI07_RS07690 0.64 -0.1 2,044,452 - RPSI07_RS07690 0.66 -1.1 2,044,487 + RPSI07_RS07690 0.68 +0.6 2,044,487 + RPSI07_RS07690 0.68 +1.1 2,044,664 + RPSI07_RS07690 0.79 -1.6 2,044,664 + RPSI07_RS07690 0.79 -3.1 2,044,664 + RPSI07_RS07690 0.79 +1.7 2,044,665 - RPSI07_RS07690 0.79 +0.2 2,044,665 - RPSI07_RS07690 0.79 +0.1 2,044,665 - RPSI07_RS07690 0.79 -0.1 2,044,665 - RPSI07_RS07690 0.79 +1.9 2,044,668 - RPSI07_RS07690 0.79 +0.2 2,044,880 - -2.3 2,045,210 + +0.5 2,045,210 + -0.7 2,045,226 - -0.2 2,045,235 - +0.4 2,045,235 - +0.1 2,045,264 + +2.3 2,045,265 - +0.1 2,045,297 + +2.7 2,045,297 + -0.3 2,045,297 + -1.7 2,045,297 + +0.5 2,045,298 - +0.3 2,045,312 + -0.1 2,045,332 + +2.9 2,045,332 + -0.0 2,045,332 + +3.2 2,045,332 + +3.7 2,045,333 - -1.1 2,045,333 - +2.9 2,045,333 - -0.3 2,045,333 - +0.3 2,045,333 - +0.9 2,045,375 - +2.0 2,045,390 + +0.2 2,045,390 + -1.5 2,045,573 + RPSI07_RS24825 0.79 -1.1 2,045,573 + RPSI07_RS24825 0.79 +3.9 2,045,573 + RPSI07_RS24825 0.79 -1.1 2,045,574 - RPSI07_RS24825 0.80 -0.1 2,045,624 + -0.7 2,045,702 + +0.1 2,045,740 - -0.9 2,045,782 + -2.5 2,045,782 + +1.4 2,045,782 + -0.1 2,045,782 + -0.1 2,045,782 + +0.4 2,045,783 - -2.0 2,045,783 - -1.5 2,045,783 - -1.0 2,045,783 - +3.2 2,045,783 - -1.8 2,045,783 - -2.5 2,045,783 - +2.2 2,045,783 - +0.8 2,045,787 + -1.6 2,045,787 + +2.9 2,045,788 - -0.7 2,045,788 - -2.1 2,045,788 - -1.5 2,045,872 - +0.5 2,045,881 + -0.1 2,045,882 - +0.1 2,045,911 + -0.5 2,045,912 - -1.4
Or see this region's nucleotide sequence